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Multiplex polymerase chain reaction
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<h2 id="applications">Applications</h2> <p>Some of the <a href="/facts/Applications_of_PCR/G0jWzJy1">applications</a> of multiplex PCR include: </p> <ol><li>Pathogen Identification<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a></li> <li>High Throughput SNP Genotyping<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a></li> <li>Mutation Analysis<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a></li> <li>Gene Deletion Analysis<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a></li> <li>Template Quantitation<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a></li> <li>Linkage Analysis<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a></li> <li>RNA Detection<a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a></li> <li>Forensic Studies<a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a></li> <li>Diet Analysis<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a></li></ol> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Chamberlain JS, Gibbs RA, Ranier JE, Nguyen PN, Caskey CT (1988). "Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification". Nucleic Acids Research. 16 (23): 11141–11156. doi:10.1093/nar/16.23.11141. PMC 339001. PMID 3205741. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339001" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339001</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Ballabio A, Ranier JE, Chamberlain JS, Zollo M, Caskey CT (1990). "Screening for steroid sulfatase (STS) gene deletions by multiplex DNA amplification" (PDF). Human Genetics. 84 (6): 571–573. doi:10.1007/BF00210812. hdl:2027.42/47626. PMID 2338343. S2CID 18579745. <a href="https://deepblue.lib.umich.edu/bitstream/2027.42/47626/1/439_2004_Article_BF00210812.pdf" target="_blank">https://deepblue.lib.umich.edu/bitstream/2027.42/47626/1/439_2004_Article_BF00210812.pdf</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008). "Multiplex-ready PCR: a new method for multiplexed SSR and SNP genotyping". BMC Genomics. 9: 80. doi:10.1186/1471-2164-9-80. PMC 2275739. PMID 18282271. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275739" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275739</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Perchetti, GA; Nalla, AK; Huang, ML; Jerome, KR; Greninger, AL (2020). "Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR". Journal of Clinical Virology. 129: 104499. doi:10.1016/j.jcv.2020.104499. PMC 7278635. PMID 32535397. <a href="/wiki/Keith_R._Jerome" target="_blank">/wiki/Keith_R._Jerome</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Järvinen, Anna-Kaarina; Laakso, Sanna; Piiparinen, Pasi; Aittakorpi, Anne; Lindfors, Merja; Huopaniemi, Laura; Piiparinen, Heli; Mäki, Minna (2009). "Rapid identification of bacterial pathogens using a PCR- and microarray-based assay". BMC Microbiology. 9: 161. doi:10.1186/1471-2180-9-161. PMC 2741468. PMID 19664269. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741468" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2741468</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Myakishev, M. V. (2001). "High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers". Genome Research. 11 (1): 163–169. doi:10.1101/gr.157901. PMC 311033. PMID 11156625. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC311033" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC311033</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Morlan, John; Baker, Joffre; Sinicropi, Dominick (2009). "Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method". PLOS ONE. 4 (2): e4584. Bibcode:2009PLoSO...4.4584M. doi:10.1371/journal.pone.0004584. PMC 2642996. PMID 19240792. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642996" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642996</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin gene". Journal of Medical Genetics. 29 (3): 191–196. doi:10.1136/jmg.29.3.191. PMC 1015896. PMID 1552558. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1015896" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1015896</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>"Welcome | Forensic DNA Profiling Facility" (PDF). <a href="http://dna.fiu.edu/Advanced%20DNA%20Typing%20lectures/An%20introduction%20to%20principles%20of%20QPCR-D.pdf" target="_blank">http://dna.fiu.edu/Advanced%20DNA%20Typing%20lectures/An%20introduction%20to%20principles%20of%20QPCR-D.pdf</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Reis, Andre (1991). "PCR in Linkage Analysis of Genetic Diseases". PCR Topics. pp. 75–79. doi:10.1007/978-3-642-75924-6_15. ISBN 978-3-540-52934-7. <a href="978-3-540-52934-7" target="_blank">978-3-540-52934-7</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> <li id="fn:11"><p>Miyakawa, Y.; Yoshizawa, H.; Mishiro, S.; Machida, A.; Akahane, Y.; Sugai, Y.; Tanaka, T.; Sugiyama, Y.; Okada, S.; Okamoto, H. (August 1990). "Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region". The Japanese Journal of Experimental Medicine. 60 (4): 215–222. PMID 1963453. <a href="/wiki/PMID_(identifier)" target="_blank">/wiki/PMID_(identifier)</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></p></li> <li id="fn:12"><p>"DNA Evidence: Basics of Analyzing". <a href="https://nij.gov/topics/forensics/evidence/dna/basics/pages/analyzing.aspx" target="_blank">https://nij.gov/topics/forensics/evidence/dna/basics/pages/analyzing.aspx</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></p></li> <li id="fn:13"><p>Dunshea, Glenn (2009). "DNA-Based Diet Analysis for Any Predator". PLOS ONE. 4 (4): e5252. Bibcode:2009PLoSO...4.5252D. doi:10.1371/journal.pone.0005252. PMC 2668750. PMID 19390570. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2668750" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2668750</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></p></li> </ol>