Originally, the strip was drawn in black-and-white, eventually became grayscale, and finally became color in June 2004.
The archive of the strip are available for free online. The strip was also syndicated free to student newspapers, with Cham earning a living through book sales, merchandise, and giving lectures.
The comic follows the lives of graduate students and their professors. Reviewer Sara Coelho said "the comic deals with everyday frustrations of life in the lab – procrastination, dealing with advisers, serving on committees, lack of inspiration". Comics deal with the isolation of graduate work.
Cham's comics have also been on real-world research, such as a five-comic series following a visit to CERN.
Cham has said that while the characters are not based on real people, he was inspired by colleagues, classmates, and his brother's friends.
Another series of strips takes up the modern debate about the proper use of the term "irony".
The comic resonated with graduate students. As of 2011, the strip's website had received 7 million visitors in the previous year, and a 2017 article reported that it received 6 million visitors a year. As of 2009, Cham had sold more than 46,000 books.
Six PhD comic books were published. These are collections of the PHD Comic strips, and some books also contain bonus material:
In March 2011, Jorge Cham started filming a movie based on the comic series. The film production was a collaboration between Cham and a theater group at the California Institute of Technology. In fall 2011, the film was released on selected academic campuses. A trailer of the movie was released to the Piled Higher and Deeper website on June 8, 2011. After about a year of various campus screenings around the world, the movie became available for purchase on DVD or streaming on April 15, 2012. A columnist for Nature wrote: "the film puts the plight of the PhD student on the big screen, giving student audiences a chance to laugh at themselves... the film tackles some of the negative aspects of pursuing a PhD and a science career... The PhD Movie raises a question that crosses many students' minds: why bother? The answer it provides resonates with the audience: Everybody is here because they want to be here ... You have to embrace the things you're passionate about.'"
The sequel to the first movie was shot in the Caltech campus. The budget of $163,000 was provided by almost Kickstarter backers. It was released in September 2015; the comedy relates to a research team seeking a key molecule in a cutthroat environment for jobs and grants.
Cham gives lectures based on his comic. A 2009 article wrote that "the popularity of his keynote lectures... are enough to make even the most distinguished professor green with envy." His first lecture was at Massachusetts Institute of Technology in 2005 and was entitled "The Power of Procrastination".
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