Menu
Home
People
Places
Arts
History
Plants & Animals
Science
Life & Culture
Technology
Reference.org
Stable isotope labeling by amino acids in cell culture
open-in-new
<h2 id="procedure">Procedure</h2> <p>Two populations of cells are cultivated in <a href="/facts/Cell_culture/7Qhu13D9">cell culture</a>. One of the cell populations is fed with <a href="/facts/Growth_medium/97IMkpFP">growth medium</a> containing normal <a href="/facts/Amino_acids/WDxYwBny">amino acids</a>. In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy <a href="/facts/Isotope/KD9B1y6o">isotopes</a>. For example, the medium can contain <a href="/facts/Arginine/y49Hdf1e">arginine</a> labeled with six <a href="/facts/Carbon-13/Iho4LUtW">carbon-13</a> atoms (13C) instead of the normal <a href="/facts/Carbon-12/nJYECzEr">carbon-12</a> (12C). When the cells are growing in this medium, they incorporate the heavy arginine into all of their proteins. Thereafter, all peptides containing a single arginine are 6 <a href="/facts/Dalton_(unit)/IMATU3vf">Da</a> heavier than their normal counterparts. Alternatively, uniform labeling with 13C or 15N can be used. Proteins from both cell populations are combined and analyzed together by <a href="/facts/Mass_spectrometry/RH5WBHJ2">mass spectrometry</a> as pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. The ratio of peak intensities in the mass spectrum for such peptide pairs reflects the abundance ratio for the two proteins.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a><a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> </p> <h2 id="applications">Applications</h2> <p>A SILAC approach involving incorporation of <a href="/facts/Tyrosine/RJhEgE4C">tyrosine</a> labeled with nine <a href="/facts/Carbon-13/Iho4LUtW">carbon-13</a> atoms (13C) instead of the normal <a href="/facts/Carbon-12/nJYECzEr">carbon-12</a> (12C) has been utilized to study tyrosine kinase substrates in signaling pathways.<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a> SILAC has emerged as a very powerful method to study <a href="/facts/Cell_signaling/6oYAVP9I">cell signaling</a>, post translation modifications such as <a href="/facts/Phosphorylation/LXUEEeIL">phosphorylation</a>,<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a><a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a> <a href="/facts/Protein%25E2%2580%2593protein_interaction/etvm9Wmg">protein–protein interaction</a> and <a href="/facts/Regulation_of_gene_expression/9N4G341I">regulation of gene expression</a>. In addition, SILAC has become an important method in <a href="/facts/Secretomics/C7dXh8uX">secretomics</a>, the global study of <a href="/facts/Secretory_protein/FI9XYhHE">secreted proteins</a> and secretory pathways.<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a> It can be used to distinguish between proteins secreted by cells in culture and serum contaminants.<a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a> It has also been adapted as a 'forward+reverse' SILAC method for simultaneous labeling of host and microbe, which enables the study of host-microbe interactions.<a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a> Standardized protocols of SILAC for various applications have also been published.<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a><a class="footnote-ref" id="fnref:14" href="#fn:14"><sup>14</sup></a> </p> <h2 id="pulsed-silac">Pulsed SILAC</h2> <p>Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. This allows monitoring differences in <i>de novo</i> protein production rather than raw concentration.<a class="footnote-ref" id="fnref:15" href="#fn:15"><sup>15</sup></a><a class="footnote-ref" id="fnref:16" href="#fn:16"><sup>16</sup></a> </p> <h2 id="neucode-silac">NeuCode SILAC</h2> <p>Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available. Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4).<a class="footnote-ref" id="fnref:17" href="#fn:17"><sup>17</sup></a> The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. The use of only heavy amino acids eliminates the need for 100% incorporation of amino acids needed for SILAC. The increased multiplexing capability of NeuCode amino acids is from the use of mass defects from extra neutrons in the stable isotopes. These small mass differences however need to be resolved on high-resolution mass spectrometers. </p> <h2 id="further-reading">Further reading</h2> <ul><li>Ong SE, Kratchmarova I, Mann M (2003). "Properties of 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC)". <i>Journal of Proteome Research</i>. 2 (2): 173–181. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1021%2Fpr0255708">10.1021/pr0255708</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/12716131">12716131</a>.</li> <li>Ong SE, Mann M (2006). "A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)". <i>Nature Protocols</i>. 1 (6): 2650–2660. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1038%2Fnprot.2006.427">10.1038/nprot.2006.427</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/17406521">17406521</a>. <a href="/facts/S2CID_(identifier)/ldJsHa2Y">S2CID</a> <a href="https://api.semanticscholar.org/CorpusID:10651610">10651610</a>.</li> <li>Ong SE, Mann M (2007). "Stable isotope labeling by amino acids in cell culture for quantitative proteomics". <i>Quantitative Proteomics by Mass Spectrometry</i>. Methods in Molecular Biology. Vol. 359. pp. 37–52. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1007%2F978-1-59745-255-7_3">10.1007/978-1-59745-255-7_3</a>. <a href="/facts/ISBN_(identifier)/15AdSPa9">ISBN</a> 978-1-58829-571-2. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/17484109">17484109</a>.</li></ul> <h2 id="external-links">External links</h2> <ul><li><a href="http://www.biochem.mpg.de/221777/SILAC">SILAC resource Mann Lab</a></li> <li><a href="http://www.silac.org/">SILAC resource Pandey Lab</a></li> <li><a href="https://web.archive.org/web/20030802092843/http://www.pil.sdu.dk/silac.htm">SILAC resource Center for Experimental Bioinformatics (CEBI)</a></li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Oda Y, Huang K, Cross FR, Cowburn D, Chait BT (June 1999). "Accurate quantitation of protein expression and site-specific phosphorylation". Proceedings of the National Academy of Sciences of the United States of America. 96 (12): 6591–6596. Bibcode:1999PNAS...96.6591O. doi:10.1073/pnas.96.12.6591. PMC 21959. PMID 10359756. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC21959" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC21959</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Jiang H, English AM (2002). "Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine". Journal of Proteome Research. 1 (4): 345–350. doi:10.1021/pr025523f. PMID 12645890. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Ong SE, Blagoev B, Kratchmarova I, Kristensen DB, Steen H, Pandey A, Mann M (May 2002). "Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics". Molecular & Cellular Proteomics. 1 (5): 376–386. doi:10.1074/mcp.M200025-MCP200. PMID 12118079. <a href="https://doi.org/10.1074%2Fmcp.M200025-MCP200" target="_blank">https://doi.org/10.1074%2Fmcp.M200025-MCP200</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Zhu H, Pan S, Gu S, Bradbury EM, Chen X (2002). "Amino acid residue specific stable isotope labeling for quantitative proteomics". Rapid Communications in Mass Spectrometry. 16 (22): 2115–2123. Bibcode:2002RCMS...16.2115Z. doi:10.1002/rcm.831. PMID 12415544. <a href="https://zenodo.org/record/1229346" target="_blank">https://zenodo.org/record/1229346</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Schoeters F, Van Dijck P (2019). "Protein-Protein Interactions in Candida albicans". Frontiers in Microbiology. 10: 1792. doi:10.3389/fmicb.2019.01792. PMC 6693483. PMID 31440220. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693483" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693483</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Ong SE, Blagoev B, Kratchmarova I, Kristensen DB, Steen H, Pandey A, Mann M (May 2002). "Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics". Molecular & Cellular Proteomics. 1 (5): 376–386. doi:10.1074/mcp.M200025-MCP200. PMID 12118079. <a href="https://doi.org/10.1074%2Fmcp.M200025-MCP200" target="_blank">https://doi.org/10.1074%2Fmcp.M200025-MCP200</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Ibarrola N, Molina H, Iwahori A, Pandey A (April 2004). "A novel proteomic approach for specific identification of tyrosine kinase substrates using [13C]tyrosine". The Journal of Biological Chemistry. 279 (16): 15805–15813. doi:10.1074/jbc.M311714200. PMID 14739304. <a href="https://doi.org/10.1074%2Fjbc.M311714200" target="_blank">https://doi.org/10.1074%2Fjbc.M311714200</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>Ibarrola N, Molina H, Iwahori A, Pandey A (April 2004). "A novel proteomic approach for specific identification of tyrosine kinase substrates using [13C]tyrosine". The Journal of Biological Chemistry. 279 (16): 15805–15813. doi:10.1074/jbc.M311714200. PMID 14739304. <a href="https://doi.org/10.1074%2Fjbc.M311714200" target="_blank">https://doi.org/10.1074%2Fjbc.M311714200</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>Ibarrola N, Kalume DE, Gronborg M, Iwahori A, Pandey A (November 2003). "A proteomic approach for quantitation of phosphorylation using stable isotope labeling in cell culture". Analytical Chemistry. 75 (22): 6043–6049. doi:10.1021/ac034931f. PMID 14615979. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Hathout Y (April 2007). "Approaches to the study of the cell secretome". Expert Review of Proteomics. 4 (2): 239–248. doi:10.1586/14789450.4.2.239. PMID 17425459. S2CID 26169223. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> <li id="fn:11"><p>Polacek M, Bruun JA, Johansen O, Martinez I (August 2010). "Differences in the secretome of cartilage explants and cultured chondrocytes unveiled by SILAC technology". Journal of Orthopaedic Research. 28 (8): 1040–1049. doi:10.1002/jor.21067. PMID 20108312. S2CID 41057768. <a href="https://doi.org/10.1002%2Fjor.21067" target="_blank">https://doi.org/10.1002%2Fjor.21067</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></p></li> <li id="fn:12"><p>Liu, Yang Sylvia; Zhang, Chengqian; Khoo, Bee Luan; Hao, Piliang; Chua, Song Lin (2024-09-02). "Dual-species proteomics and targeted intervention of animal-pathogen interactions". Journal of Advanced Research. doi:10.1016/j.jare.2024.08.038. ISSN 2090-1232. <a href="https://www.sciencedirect.com/science/article/pii/S2090123224003837" target="_blank">https://www.sciencedirect.com/science/article/pii/S2090123224003837</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></p></li> <li id="fn:13"><p>Amanchy R, Kalume DE, Pandey A (January 2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying dynamics of protein abundance and posttranslational modifications". Science's STKE. 2005 (267): pl2. doi:10.1126/stke.2672005pl2. PMID 15657263. S2CID 12089034. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></p></li> <li id="fn:14"><p>Harsha HC, Molina H, Pandey A (2008). "Quantitative proteomics using stable isotope labeling with amino acids in cell culture". Nature Protocols. 3 (3): 505–516. doi:10.1038/nprot.2008.2. PMID 18323819. S2CID 24190501. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></p></li> <li id="fn:15"><p>Schwanhäusser B, Gossen M, Dittmar G, Selbach M (January 2009). "Global analysis of cellular protein translation by pulsed SILAC". Proteomics. 9 (1): 205–209. doi:10.1002/pmic.200800275. PMID 19053139. S2CID 23130202. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:15" class="footnote-back-ref">↩</a></p></li> <li id="fn:16"><p>Chua, Song Lin; Yam, Joey Kuok Hoong; Hao, Piliang; Adav, Sunil S.; Salido, May Margarette; Liu, Yang; Givskov, Michael; Sze, Siu Kwan; Tolker-Nielsen, Tim; Yang, Liang (2016-02-19). "Selective labelling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms". Nature Communications. 7 (1): 10750. doi:10.1038/ncomms10750. ISSN 2041-1723. PMC 4762895. <a href="https://www.nature.com/articles/ncomms10750" target="_blank">https://www.nature.com/articles/ncomms10750</a> <a href="#fnref:16" class="footnote-back-ref">↩</a></p></li> <li id="fn:17"><p>Merrill AE, Hebert AS, MacGilvray ME, Rose CM, Bailey DJ, Bradley JC, et al. (September 2014). "NeuCode labels for relative protein quantification". Molecular & Cellular Proteomics. 13 (9): 2503–2512. doi:10.1074/mcp.M114.040287. PMC 4159665. PMID 24938287. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159665" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159665</a> <a href="#fnref:17" class="footnote-back-ref">↩</a></p></li> </ol>