DNA nanotechnology

<h2 id="history">History</h2>
The conceptual foundation for DNA nanotechnology was first laid out by <a href="/facts/Nadrian_Seeman/uRmyzmDm">Nadrian Seeman</a> in the early 1980s.<a class="footnote-ref" id="fnref:1" href="#fn:1">1</a> Seeman's original motivation was to create a three-dimensional DNA lattice for orienting other large molecules, which would simplify their <a href="/facts/Crystallography/9B5toeqG">crystallographic study</a> by eliminating the difficult process of obtaining pure crystals. This idea had reportedly come to him in late 1980, after realizing the similarity between the woodcut Depth by <a href="/facts/M._C._Escher/6uuAGlDx">M. C. Escher</a> and an array of DNA six-arm junctions.<a class="footnote-ref" id="fnref:2" href="#fn:2">2</a><a class="footnote-ref" id="fnref:3" href="#fn:3">3</a> Several natural branched DNA structures were known at the time, including the DNA <a href="/facts/Replication_fork/wrU6RuR3">replication fork</a> and the mobile <a href="/facts/Holliday_junction/7P2GBz10">Holliday junction</a>, but Seeman's insight was that immobile nucleic acid junctions could be created by properly designing the strand sequences to remove symmetry in the assembled molecule, and that these immobile junctions could in principle be combined into rigid crystalline lattices. The first theoretical paper proposing this scheme was published in 1982, and the first experimental demonstration of an immobile DNA junction was published the following year.<a class="footnote-ref" id="fnref:4" href="#fn:4">4</a><a class="footnote-ref" id="fnref:5" href="#fn:5">5</a>

In 1991, Seeman's laboratory published a report on the synthesis of a cube made of DNA, the first synthetic three-dimensional nucleic acid nanostructure, for which he received the 1995 <a href="/facts/Foresight_Institute_Feynman_Prize_in_Nanotechnology/XoGhLKqH">Feynman Prize in Nanotechnology</a>. This was followed by a DNA <a href="/facts/Truncated_octahedron/GA98b4TH">truncated octahedron</a>. It soon became clear that these structures, polygonal shapes with flexible junctions as their <a href="/facts/Vertex_(geometry)/ID3cea9z">vertices</a>, were not rigid enough to form extended three-dimensional lattices. Seeman developed the more rigid double-crossover (DX) <a href="/facts/Structural_motif/g8EBDBGW">structural motif</a>, and in 1998, in collaboration with <a href="/facts/Erik_Winfree/6MRyR3Lz">Erik Winfree</a>, published the creation of two-dimensional lattices of DX tiles.<a class="footnote-ref" id="fnref:6" href="#fn:6">6</a><a class="footnote-ref" id="fnref:7" href="#fn:7">7</a><a class="footnote-ref" id="fnref:8" href="#fn:8">8</a> These tile-based structures had the advantage that they provided the ability to implement DNA computing, which was demonstrated by Winfree and <a href="/facts/Paul_Rothemund/MGSjn4HG">Paul Rothemund</a> in their 2004 paper on the algorithmic self-assembly of a Sierpinski gasket structure, and for which they shared the 2006 Feynman Prize in Nanotechnology. Winfree's key insight was that the DX tiles could be used as <a href="/facts/Wang_tile/639AJElj">Wang tiles</a>, meaning that their assembly could perform computation.<a class="footnote-ref" id="fnref:9" href="#fn:9">9</a> The synthesis of a three-dimensional lattice was finally published by Seeman in 2009, nearly thirty years after he had set out to achieve it.<a class="footnote-ref" id="fnref:10" href="#fn:10">10</a>
New abilities continued to be discovered for designed DNA structures throughout the 2000s. The first <a href="/facts/DNA_machine/Jvdy0NuK">DNA nanomachine</a>—a motif that changes its structure in response to an input—was demonstrated in 1999 by Seeman. An improved system, which was the first nucleic acid device to make use of toehold-mediated strand displacement, was demonstrated by <a href="/facts/Bernard_Yurke/1QLN9n2k">Bernard Yurke</a> in 2000.<a class="footnote-ref" id="fnref:11" href="#fn:11">11</a> The next advance was to translate this into mechanical motion, and in 2004 and 2005, several DNA walker systems were demonstrated by the groups of Seeman, <a href="/facts/Niles_Pierce/523L1qvx">Niles Pierce</a>, <a href="/facts/Andrew_Turberfield/Jtq24KHQ">Andrew Turberfield</a>, and Chengde Mao.<a class="footnote-ref" id="fnref:12" href="#fn:12">12</a> The idea of using DNA arrays to template the assembly of other molecules such as nanoparticles and proteins, first suggested by Bruche Robinson and Seeman in 1987,<a class="footnote-ref" id="fnref:13" href="#fn:13">13</a> was demonstrated in 2002 by Seeman, Kiehl et al.<a class="footnote-ref" id="fnref:14" href="#fn:14">14</a> and subsequently by many other groups.
In 2006, Rothemund first demonstrated the <a href="/facts/DNA_origami/Ce9Cv85M">DNA origami</a> method for easily and robustly forming folded DNA structures of arbitrary shape. Rothemund had conceived of this method as being conceptually intermediate between Seeman's DX lattices, which used many short strands, and William Shih's DNA octahedron, which consisted mostly of one very long strand. Rothemund's DNA origami contains a long strand which folding is assisted by several short strands. This method allowed forming much larger structures than formerly possible, and which are less technically demanding to design and synthesize.<a class="footnote-ref" id="fnref:15" href="#fn:15">15</a> DNA origami was the cover story of <a href="/facts/Nature_(journal)/XmlJXkFx">Nature</a> on March 15, 2006.<a class="footnote-ref" id="fnref:16" href="#fn:16">16</a> Rothemund's research demonstrating two-dimensional DNA origami structures was followed by the demonstration of solid three-dimensional DNA origami by Douglas et al. in 2009,<a class="footnote-ref" id="fnref:17" href="#fn:17">17</a> while the labs of Jørgen Kjems and Yan demonstrated hollow three-dimensional structures made out of two-dimensional faces.<a class="footnote-ref" id="fnref:18" href="#fn:18">18</a>
DNA nanotechnology was initially met with some skepticism due to the unusual non-biological use of nucleic acids as materials for building structures and doing computation, and the preponderance of <a href="/facts/Proof_of_principle/1YzUH8LJ">proof of principle</a> experiments that extended the abilities of the field but were far from actual applications. Seeman's 1991 paper on the synthesis of the DNA cube was rejected by the journal <a href="/facts/Science_(journal)/uTyzjkwD">Science</a> after one reviewer praised its originality while another criticized it for its lack of biological relevance.<a class="footnote-ref" id="fnref:19" href="#fn:19">19</a> By the early 2010s the field was considered to have increased its abilities to the point that applications for basic science research were beginning to be realized, and practical applications in medicine and other fields were beginning to be considered feasible.<a class="footnote-ref" id="fnref:20" href="#fn:20">20</a><a class="footnote-ref" id="fnref:21" href="#fn:21">21</a> The field had grown from very few active laboratories in 2001 to at least 60 in 2010, which increased the talent pool and thus the number of scientific advances in the field during that decade.<a class="footnote-ref" id="fnref:22" href="#fn:22">22</a>

<h2 id="fundamental-concepts">Fundamental concepts</h2>

<h3>Properties of nucleic acids</h3>
<a href="/facts/Nanotechnology/Co4FFNfY">Nanotechnology</a> is often defined as the study of materials and devices with features on a scale below 100 <a href="/facts/Nanometer/a98sjHyu">nanometers</a>. DNA nanotechnology, specifically, is an example of <a href="/facts/Top-down_and_bottom-up_design/MvtTkA3x">bottom-up</a> <a href="/facts/Molecular_self-assembly/DhU6CEYL">molecular self-assembly</a>, in which molecular components spontaneously organize into stable structures; the particular form of these structures is induced by the physical and chemical properties of the components selected by the designers.<a class="footnote-ref" id="fnref:23" href="#fn:23">23</a> In DNA nanotechnology, the component materials are strands of nucleic acids such as DNA; these strands are often synthetic and are almost always used outside the context of a living cell. DNA is well-suited to nanoscale construction because the binding between two nucleic acid strands depends on simple <a href="/facts/Base_pair/xmPpPQ9W">base pairing</a> rules which are well understood, and form the specific nanoscale structure of the <a href="/facts/Nucleic_acid_double_helix/XHeMuslB">nucleic acid double helix</a>. These qualities make the assembly of nucleic acid structures easy to control through <a href="/facts/Nucleic_acid_design/WC4DFGNQ">nucleic acid design</a>. This property is absent in other materials used in nanotechnology, including <a href="/facts/Protein/Jxmagu3E">proteins</a>, for which <a href="/facts/Protein_design/jFKIHFvc">protein design</a> is very difficult, and <a href="/facts/Nanoparticle/k8kNxX4E">nanoparticles</a>, which lack the capability for specific assembly on their own.<a class="footnote-ref" id="fnref:24" href="#fn:24">24</a>
The <a href="/facts/Nucleic_acid_structure/U7sYh5pn">structure</a> of a nucleic acid molecule consists of a sequence of <a href="/facts/Nucleotide/pQKEwlJI">nucleotides</a> distinguished by which <a href="/facts/Nucleobase/zj1Vl9Qh">nucleobase</a> they contain. In DNA, the four bases present are <a href="/facts/Adenine/PSeDFBnl">adenine</a> (A), <a href="/facts/Cytosine/JW20j33u">cytosine</a> (C), <a href="/facts/Guanine/hoTSpz0x">guanine</a> (G), and <a href="/facts/Thymine/A5C8MnLN">thymine</a> (T). Nucleic acids have the property that two molecules will only bind to each other to form a double helix if the two sequences are <a href="/facts/Complementarity_(molecular_biology)/WQfqGlBJ">complementary</a>, meaning that they form matching sequences of base pairs, with A only binding to T, and C only to G.<a class="footnote-ref" id="fnref:25" href="#fn:25">25</a><a class="footnote-ref" id="fnref:26" href="#fn:26">26</a> Because the formation of correctly matched base pairs is <a href="/facts/Nucleic_acid_thermodynamics/4YD7V7hM">energetically favorable</a>, nucleic acid strands are expected in most cases to bind to each other in the conformation that maximizes the number of correctly paired bases. The sequences of bases in a system of strands thus determine the pattern of binding and the overall structure in an easily controllable way. In DNA nanotechnology, the base sequences of strands are rationally designed by researchers so that the base pairing interactions cause the strands to assemble in the desired conformation.<a class="footnote-ref" id="fnref:27" href="#fn:27">27</a><a class="footnote-ref" id="fnref:28" href="#fn:28">28</a> While <a href="/facts/DNA/nB89Iauz">DNA</a> is the dominant material used, structures incorporating other nucleic acids such as <a href="/facts/RNA/HxPeNfNj">RNA</a> and <a href="/facts/Peptide_nucleic_acid/9MkVaqXK">peptide nucleic acid</a> (PNA) have also been constructed.<a class="footnote-ref" id="fnref:29" href="#fn:29">29</a><a class="footnote-ref" id="fnref:30" href="#fn:30">30</a>

<h3>Subfields</h3>
DNA nanotechnology is sometimes divided into two overlapping subfields: structural DNA nanotechnology and dynamic DNA nanotechnology. Structural DNA nanotechnology, sometimes abbreviated as SDN, focuses on synthesizing and characterizing nucleic acid complexes and materials that assemble into a static, <a href="/facts/Chemical_equilibrium/y8ToYXHp">equilibrium</a> end state. On the other hand, dynamic DNA nanotechnology focuses on complexes with useful non-equilibrium behavior such as the ability to reconfigure based on a chemical or physical stimulus. Some complexes, such as nucleic acid nanomechanical devices, combine features of both the structural and dynamic subfields.<a class="footnote-ref" id="fnref:31" href="#fn:31">31</a><a class="footnote-ref" id="fnref:32" href="#fn:32">32</a>
The complexes constructed in structural DNA nanotechnology use topologically branched nucleic acid structures containing junctions. (In contrast, most biological DNA exists as an unbranched <a href="/facts/Nucleic_acid_double_helix/XHeMuslB">double helix</a>.) One of the simplest branched structures is a four-arm junction that consists of four individual DNA strands, portions of which are complementary in a specific pattern. Unlike in natural <a href="/facts/Holliday_junction/7P2GBz10">Holliday junctions</a>, each arm in the artificial immobile four-arm junction has a different <a href="/facts/Nucleic_acid_sequence/qAf4kTee">base sequence</a>, causing the junction point to be fixed at a certain position. Multiple junctions can be combined in the same complex, such as in the widely used double-crossover (DX) <a href="/facts/Structural_motif/g8EBDBGW">structural motif</a>, which contains two parallel double helical domains with individual strands crossing between the domains at two crossover points. Each crossover point is, topologically, a four-arm junction, but is constrained to one orientation, in contrast to the flexible single four-arm junction, providing a rigidity that makes the DX motif suitable as a structural building block for larger DNA complexes.<a class="footnote-ref" id="fnref:33" href="#fn:33">33</a><a class="footnote-ref" id="fnref:34" href="#fn:34">34</a>
Dynamic DNA nanotechnology uses a mechanism called <a href="/facts/Toehold_mediated_strand_displacement/XdeKuWl5">toehold-mediated strand displacement</a> to allow the nucleic acid complexes to reconfigure in response to the addition of a new nucleic acid strand. In this reaction, the incoming strand binds to a <a href="/facts/Sticky_end/cKcexPfJ">single-stranded toehold region</a> of a double-stranded complex, and then displaces one of the strands bound in the original complex through a <a href="/facts/Branch_migration/91QTMeSg">branch migration</a> process. The overall effect is that one of the strands in the complex is replaced with another one.<a class="footnote-ref" id="fnref:35" href="#fn:35">35</a> In addition, reconfigurable structures and devices can be made using functional nucleic acids such as <a href="/facts/Deoxyribozyme/c7ApejX4">deoxyribozymes</a> and <a href="/facts/Ribozyme/yvcl3ygp">ribozymes</a>, which can perform chemical reactions, and <a href="/facts/Aptamer/3UvhuQP4">aptamers</a>, which can bind to specific proteins or small molecules.<a class="footnote-ref" id="fnref:36" href="#fn:36">36</a>

<h2 id="structural-dna-nanotechnology">Structural DNA nanotechnology</h2>
Structural DNA nanotechnology, sometimes abbreviated as SDN, focuses on synthesizing and characterizing nucleic acid complexes and materials where the assembly has a static, equilibrium endpoint. The <a href="/facts/Nucleic_acid_double_helix/XHeMuslB">nucleic acid double helix</a> has a robust, defined three-dimensional geometry that makes it possible to simulate,<a class="footnote-ref" id="fnref:37" href="#fn:37">37</a> predict and design the structures of more complicated nucleic acid complexes. Many such structures have been created, including two- and three-dimensional structures, and periodic, aperiodic, and discrete structures.<a class="footnote-ref" id="fnref:38" href="#fn:38">38</a>

<h3>Extended lattices</h3>

Small nucleic acid complexes can be equipped with <a href="/facts/Sticky_end/cKcexPfJ">sticky ends</a> and combined into larger two-dimensional periodic lattices containing a specific <a href="/facts/Tessellation/7BWDywXC">tessellated</a> pattern of the individual molecular tiles.<a class="footnote-ref" id="fnref:39" href="#fn:39">39</a> The earliest example of this used double-crossover (DX) complexes as the basic tiles, each containing four sticky ends designed with sequences that caused the DX units to combine into periodic two-dimensional flat sheets that are essentially rigid two-dimensional crystals of DNA.<a class="footnote-ref" id="fnref:40" href="#fn:40">40</a><a class="footnote-ref" id="fnref:41" href="#fn:41">41</a> Two-dimensional arrays have been made from other motifs as well, including the <a href="/facts/Holliday_junction/7P2GBz10">Holliday junction</a> <a href="/facts/Rhombus/T4pU50GF">rhombus</a> lattice,<a class="footnote-ref" id="fnref:42" href="#fn:42">42</a> and various DX-based arrays making use of a double-cohesion scheme.<a class="footnote-ref" id="fnref:43" href="#fn:43">43</a><a class="footnote-ref" id="fnref:44" href="#fn:44">44</a> The top two images at right show examples of tile-based periodic lattices.
Two-dimensional arrays can be made to exhibit aperiodic structures whose assembly implements a specific algorithm, exhibiting one form of DNA computing.<a class="footnote-ref" id="fnref:45" href="#fn:45">45</a> The DX tiles can have their sticky end sequences chosen so that they act as <a href="/facts/Wang_tile/639AJElj">Wang tiles</a>, allowing them to perform computation. A DX array whose assembly encodes an <a href="/facts/Exclusive_or/FHyr7hGQ">XOR</a> operation has been demonstrated; this allows the DNA array to implement a <a href="/facts/Cellular_automaton/6gfkjKAW">cellular automaton</a> that generates a <a href="/facts/Fractal/sXj38JeN">fractal</a> known as the <a href="/facts/Sierpinski_gasket/gFmLRJxM">Sierpinski gasket</a>. The third image at right shows this type of array.<a class="footnote-ref" id="fnref:46" href="#fn:46">46</a> Another system has the function of a binary <a href="/facts/Counter_(digital)/SD2V8R8w">counter</a>, displaying a representation of increasing binary numbers as it grows. These results show that computation can be incorporated into the assembly of DNA arrays.<a class="footnote-ref" id="fnref:47" href="#fn:47">47</a>
DX arrays have been made to form hollow nanotubes 4–20 <a href="/facts/Nanometer/a98sjHyu">nm</a> in diameter, essentially two-dimensional lattices which curve back upon themselves.<a class="footnote-ref" id="fnref:48" href="#fn:48">48</a> These DNA nanotubes are somewhat similar in size and shape to <a href="/facts/Carbon_nanotube/atuDPPaG">carbon nanotubes</a>, and while they lack the electrical conductance of carbon nanotubes, DNA nanotubes are more easily modified and connected to other structures. One of many schemes for constructing DNA nanotubes uses a lattice of curved DX tiles that curls around itself and closes into a tube.<a class="footnote-ref" id="fnref:49" href="#fn:49">49</a> In an alternative method that allows the circumference to be specified in a simple, modular fashion using single-stranded tiles, the rigidity of the tube is an <a href="/facts/Emergent_property/gLAd6wda">emergent property</a>.<a class="footnote-ref" id="fnref:50" href="#fn:50">50</a>
Forming three-dimensional lattices of DNA was the earliest goal of DNA nanotechnology, but this proved to be one of the most difficult to realize. Success using a motif based on the concept of <a href="/facts/Tensegrity/9j5g7GRB">tensegrity</a>, a balance between tension and compression forces, was finally reported in 2009.<a class="footnote-ref" id="fnref:51" href="#fn:51">51</a><a class="footnote-ref" id="fnref:52" href="#fn:52">52</a>

<h3>Discrete structures</h3>
Researchers have synthesized many three-dimensional DNA complexes that each have the connectivity of a <a href="/facts/Polyhedron/UC0p9FTF">polyhedron</a>, such as a <a href="/facts/Cube/65lUaAqN">cube</a> or <a href="/facts/Octahedron/G38q92xW">octahedron</a>, meaning that the DNA duplexes trace the <a href="/facts/Edge_(geometry)/3lFN6sEz">edges</a> of a polyhedron with a DNA junction at each vertex.<a class="footnote-ref" id="fnref:53" href="#fn:53">53</a> The earliest demonstrations of DNA polyhedra were very work-intensive, requiring multiple <a href="/facts/DNA_ligase/gvgKYAJD">ligations</a> and <a href="/facts/Solid-phase_synthesis/f0GX3j9W">solid-phase synthesis</a> steps to create <a href="/facts/Catenane/843mxBaf">catenated</a> polyhedra.<a class="footnote-ref" id="fnref:54" href="#fn:54">54</a> Subsequent work yielded polyhedra whose synthesis was much easier. These include a DNA octahedron made from a long single strand designed to fold into the correct conformation,<a class="footnote-ref" id="fnref:55" href="#fn:55">55</a> and a tetrahedron that can be produced from four DNA strands in one step, pictured at the top of this article.<a class="footnote-ref" id="fnref:56" href="#fn:56">56</a>
Nanostructures of arbitrary, non-regular shapes are usually made using the <a href="/facts/DNA_origami/Ce9Cv85M">DNA origami</a> method. These structures consist of a long, natural virus strand as a "scaffold", which is made to fold into the desired shape by computationally designed short "staple" strands. This method has the advantages of being easy to design, as the <a href="/facts/Nucleic_acid_sequence/qAf4kTee">base sequence</a> is predetermined by the scaffold strand sequence, and not requiring high strand purity and accurate <a href="/facts/Stoichiometry/I5p9cJzV">stoichiometry</a>, as most other DNA nanotechnology methods do. DNA origami was first demonstrated for two-dimensional shapes, such as a <a href="/facts/Smiley/4aS92RNw">smiley face</a>, a coarse map of the Western Hemisphere, and the Mona Lisa painting.<a class="footnote-ref" id="fnref:57" href="#fn:57">57</a><a class="footnote-ref" id="fnref:58" href="#fn:58">58</a><a class="footnote-ref" id="fnref:59" href="#fn:59">59</a> Solid three-dimensional structures can be made by using parallel DNA helices arranged in a honeycomb pattern,<a class="footnote-ref" id="fnref:60" href="#fn:60">60</a> and structures with two-dimensional faces can be made to fold into a hollow overall three-dimensional shape, akin to a cardboard box. These can be programmed to open and reveal or release a molecular cargo in response to a stimulus, making them potentially useful as programmable <a href="/facts/Molecular_encapsulation/2xQSjRb5">molecular cages</a>.<a class="footnote-ref" id="fnref:61" href="#fn:61">61</a><a class="footnote-ref" id="fnref:62" href="#fn:62">62</a>

<h3>Templated assembly</h3>
Nucleic acid structures can be made to incorporate molecules other than nucleic acids, sometimes called heteroelements, including proteins, metallic nanoparticles, <a href="/facts/Quantum_dot/K5Ytv8Yw">quantum dots</a>, <a href="/facts/Amine/JvfYJfP6">amines</a>,<a class="footnote-ref" id="fnref:63" href="#fn:63">63</a> and <a href="/facts/Fullerene/LPdnl8ox">fullerenes</a>. This allows the construction of materials and devices with a range of functionalities much greater than is possible with nucleic acids alone. The goal is to use the self-assembly of the nucleic acid structures to template the assembly of the nanoparticles hosted on them, controlling their position and in some cases orientation.<a class="footnote-ref" id="fnref:64" href="#fn:64">64</a><a class="footnote-ref" id="fnref:65" href="#fn:65">65</a>
Many of these schemes use a covalent attachment scheme, using oligonucleotides with <a href="/facts/Amide/C6kCzYEA">amide</a> or <a href="/facts/Thiol/VemKEDP9">thiol</a> functional groups as a chemical handle to bind the heteroelements. This covalent binding scheme has been used to arrange <a href="/facts/Gold_nanoparticle/AvLN368l">gold nanoparticles</a> on a DX-based array,<a class="footnote-ref" id="fnref:66" href="#fn:66">66</a>
and to arrange <a href="/facts/Streptavidin/7CxGjCz1">streptavidin</a> protein molecules into specific patterns on a DX array.<a class="footnote-ref" id="fnref:67" href="#fn:67">67</a>
A non-covalent hosting scheme using <a href="/facts/Peter_Dervan/P1ret8Jm">Dervan</a> polyamides on a DX array was used to arrange streptavidin proteins in a specific pattern on a DX array.<a class="footnote-ref" id="fnref:68" href="#fn:68">68</a> Carbon nanotubes have been hosted on DNA arrays in a pattern allowing the assembly to act as a <a href="/facts/Molecular_scale_electronics/XqRyCtsU">molecular electronic</a> device, a <a href="/facts/Carbon_nanotube_field-effect_transistor/EVQmIUak">carbon nanotube field-effect transistor</a>.<a class="footnote-ref" id="fnref:69" href="#fn:69">69</a> In addition, there are nucleic acid metallization methods, in which the nucleic acid is replaced by a metal which assumes the general shape of the original nucleic acid structure,<a class="footnote-ref" id="fnref:70" href="#fn:70">70</a> and schemes for using nucleic acid nanostructures as <a href="/facts/Lithography/LFwpJbE7">lithography</a> masks, transferring their pattern into a solid surface.<a class="footnote-ref" id="fnref:71" href="#fn:71">71</a>

<h2 id="dynamic-dna-nanotechnology">Dynamic DNA nanotechnology</h2>

Dynamic DNA nanotechnology focuses on forming nucleic acid systems with designed dynamic functionalities related to their overall structures, such as computation and mechanical motion. There is some overlap between structural and dynamic DNA nanotechnology, as structures can be formed through annealing and then reconfigured dynamically, or can be made to form dynamically in the first place.<a class="footnote-ref" id="fnref:72" href="#fn:72">72</a><a class="footnote-ref" id="fnref:73" href="#fn:73">73</a>

<h3>Nanomechanical devices</h3>
Main article: <a href="/facts/DNA_machine/Jvdy0NuK">DNA machine</a>
DNA complexes have been made that change their conformation upon some stimulus, making them one form of <a href="/facts/Nanorobotics/LmFlh2IJ">nanorobotics</a>. These structures are initially formed in the same way as the static structures made in structural DNA nanotechnology, but are designed so that dynamic reconfiguration is possible after the initial assembly.<a class="footnote-ref" id="fnref:74" href="#fn:74">74</a><a class="footnote-ref" id="fnref:75" href="#fn:75">75</a> The earliest such device made use of the transition between the <a href="/facts/B-DNA/XHeMuslB">B-DNA</a> and <a href="/facts/Z-DNA/9rqBUqN7">Z-DNA</a> forms to respond to a change in <a href="/facts/Buffer_solution/zrLz9eaa">buffer</a> conditions by undergoing a twisting motion.<a class="footnote-ref" id="fnref:76" href="#fn:76">76</a>
This reliance on buffer conditions caused all devices to change state at the same time. Subsequent systems could change states based upon the presence of control strands, allowing multiple devices to be independently operated in solution. Some examples of such systems are a "molecular tweezers" design that has an open and a closed state,<a class="footnote-ref" id="fnref:77" href="#fn:77">77</a> a device that could switch from a paranemic-crossover (PX) conformation to a (JX2) conformation with two non-junction juxtapositions of the DNA backbone, undergoing rotational motion in the process,<a class="footnote-ref" id="fnref:78" href="#fn:78">78</a> and a two-dimensional array that could dynamically expand and contract in response to control strands.<a class="footnote-ref" id="fnref:79" href="#fn:79">79</a> Structures have also been made that dynamically open or close, potentially acting as a molecular cage to release or reveal a functional cargo upon opening.<a class="footnote-ref" id="fnref:80" href="#fn:80">80</a><a class="footnote-ref" id="fnref:81" href="#fn:81">81</a><a class="footnote-ref" id="fnref:82" href="#fn:82">82</a> In another example, a DNA origami nanostructure was coupled to T7 RNA polymerase and could thus be operated as a chemical energy-driven motor that can be coupled to a passive follower, which it then drives.<a class="footnote-ref" id="fnref:83" href="#fn:83">83</a>
<a href="/facts/DNA_walker/fPw9EPGe">DNA walkers</a> are a class of nucleic acid nanomachines that exhibit directional motion along a linear track. A large number of schemes have been demonstrated.<a class="footnote-ref" id="fnref:84" href="#fn:84">84</a> One strategy is to control the motion of the walker along the track using control strands that need to be manually added in sequence.<a class="footnote-ref" id="fnref:85" href="#fn:85">85</a><a class="footnote-ref" id="fnref:86" href="#fn:86">86</a> It is also possible to control individual steps of a DNA walker by irradiation with light of different wavelengths.<a class="footnote-ref" id="fnref:87" href="#fn:87">87</a> Another approach is to make use of <a href="/facts/Restriction_enzyme/Mg0VgkCX">restriction enzymes</a> or <a href="/facts/Deoxyribozyme/c7ApejX4">deoxyribozymes</a> to cleave the strands and cause the walker to move forward, which has the advantage of running autonomously.<a class="footnote-ref" id="fnref:88" href="#fn:88">88</a><a class="footnote-ref" id="fnref:89" href="#fn:89">89</a> A later system could walk upon a two-dimensional surface rather than a linear track, and demonstrated the ability to selectively pick up and move molecular cargo.<a class="footnote-ref" id="fnref:90" href="#fn:90">90</a> In 2018, a <a href="/facts/Catenation/vm7k4zsv">catenated</a> DNA that uses rolling circle transcription by an attached <a href="/facts/T7_RNA_polymerase/zo6JXeIp">T7 RNA polymerase</a> was shown to walk along a DNA-path, guided by the generated RNA strand.<a class="footnote-ref" id="fnref:91" href="#fn:91">91</a> Additionally, a linear walker has been demonstrated that performs <a href="/facts/Nucleic_acid_templated_chemistry/09RSurKE">DNA-templated synthesis</a> as the walker advances along the track, allowing autonomous multistep chemical synthesis directed by the walker.<a class="footnote-ref" id="fnref:92" href="#fn:92">92</a> The synthetic DNA walkers' function is similar to that of the proteins dynein and kinesin.<a class="footnote-ref" id="fnref:93" href="#fn:93">93</a>

<h3>Strand displacement cascades</h3>
Cascades of strand displacement reactions can be used for either computational or structural purposes. An individual strand displacement reaction involves revealing a new sequence in response to the presence of some initiator strand. Many such reactions can be linked into a <a href="/facts/Biochemical_cascade/bCsA7XXG">cascade</a> where the newly revealed output sequence of one reaction can initiate another strand displacement reaction elsewhere. This in turn allows for the construction of chemical reaction networks with many components, exhibiting complex computational and information processing abilities. These cascades are made energetically favorable through the formation of new base pairs, and the <a href="/facts/Entropy/s52IXeFz">entropy</a> gain from disassembly reactions. Strand displacement cascades allow isothermal operation of the assembly or computational process, in contrast to traditional nucleic acid assembly's requirement for a thermal annealing step, where the temperature is raised and then slowly lowered to ensure proper formation of the desired structure. They can also support <a href="/facts/Catalysis/LPBS7TQC">catalytic</a> function of the initiator species, where less than one equivalent of the initiator can cause the reaction to go to completion.<a class="footnote-ref" id="fnref:94" href="#fn:94">94</a><a class="footnote-ref" id="fnref:95" href="#fn:95">95</a>
Strand displacement complexes can be used to make <a href="/facts/Molecular_logic_gate/Hoasat7d">molecular logic gates</a> capable of complex computation.<a class="footnote-ref" id="fnref:96" href="#fn:96">96</a> Unlike traditional electronic computers, which use <a href="/facts/Electric_current/Dc1xdnUX">electric current</a> as inputs and outputs, molecular computers use the concentrations of specific chemical species as signals. In the case of nucleic acid strand displacement circuits, the signal is the presence of nucleic acid strands that are released or consumed by binding and unbinding events to other strands in displacement complexes. This approach has been used to make <a href="/facts/Logic_gates/RHLulvKI">logic gates</a> such as AND, OR, and NOT gates.<a class="footnote-ref" id="fnref:97" href="#fn:97">97</a> More recently, a four-bit circuit was demonstrated that can compute the <a href="/facts/Square_root/AfrzfBdQ">square root</a> of the integers 0–15, using a system of gates containing 130 DNA strands.<a class="footnote-ref" id="fnref:98" href="#fn:98">98</a>
Another use of strand displacement cascades is to make dynamically assembled structures. These use a <a href="/facts/Stem-loop/GysAzrX5">hairpin</a> structure for the reactants, so that when the input strand binds, the newly revealed sequence is on the same molecule rather than disassembling. This allows new opened hairpins to be added to a growing complex. This approach has been used to make simple structures such as three- and four-arm junctions and <a href="/facts/Dendrimer/3aXUKBej">dendrimers</a>.<a class="footnote-ref" id="fnref:99" href="#fn:99">99</a>

<h2 id="applications">Applications</h2>
DNA nanotechnology provides one of the few ways to form designed, complex structures with precise control over nanoscale features. The field is beginning to see application to solve <a href="/facts/Basic_science/QvrBkrRb">basic science</a> problems in <a href="/facts/Structural_biology/XRkPtHYv">structural biology</a> and <a href="/facts/Biophysics/l9u5gqCy">biophysics</a>. The earliest such application envisaged for the field, and one still in development, is in <a href="/facts/Crystallography/9B5toeqG">crystallography</a>, where molecules that are difficult to crystallize in isolation could be arranged within a three-dimensional nucleic acid lattice, allowing determination of their structure. Another application is the use of <a href="/facts/DNA_origami/Ce9Cv85M">DNA origami</a> rods to replace <a href="/facts/Liquid_crystals/ehCCST89">liquid crystals</a> in <a href="/facts/Residual_dipolar_coupling/m1SV2Bsz">residual dipolar coupling</a> experiments in <a href="/facts/Nuclear_magnetic_resonance_spectroscopy_of_proteins/lCIXAFzQ">protein NMR spectroscopy</a>; using DNA origami is advantageous because, unlike liquid crystals, they are tolerant of the detergents needed to suspend <a href="/facts/Membrane_protein/FboTeM8Q">membrane proteins</a> in solution. DNA walkers have been used as nanoscale assembly lines to move nanoparticles and direct <a href="/facts/Chemical_synthesis/3KXsVVwC">chemical synthesis</a>. Further, DNA origami structures have aided in the biophysical studies of <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a> function and <a href="/facts/Protein_folding/rf3nR2Y1">protein folding</a>.<a class="footnote-ref" id="fnref:100" href="#fn:100">100</a><a class="footnote-ref" id="fnref:101" href="#fn:101">101</a>
DNA nanotechnology is moving toward potential real-world applications. The ability of nucleic acid arrays to arrange other molecules indicates its potential applications in molecular scale electronics. The assembly of a nucleic acid structure could be used to template the assembly of molecular electronic elements such as <a href="/facts/Molecular_wire/E7dSB6mF">molecular wires</a>, providing a method for nanometer-scale control of the placement and overall architecture of the device analogous to a molecular <a href="/facts/Breadboard/fokXRhUn">breadboard</a>.<a class="footnote-ref" id="fnref:102" href="#fn:102">102</a><a class="footnote-ref" id="fnref:103" href="#fn:103">103</a> DNA nanotechnology has been compared to the concept of <a href="/facts/Programmable_matter/18hvSS3L">programmable matter</a> because of the coupling of computation to its material properties.<a class="footnote-ref" id="fnref:104" href="#fn:104">104</a>
In a study conducted by a group of scientists from <a href="/facts/Interdisciplinary_Nanoscience_Center/Y9zjJXCt">iNANO</a> and CDNA centers in <a href="/facts/Aarhus_University/HV9RTWYH">Aarhus University</a>, researchers were able to construct a small multi-switchable 3D DNA Box Origami. The proposed nanoparticle was characterized by <a href="/facts/Atomic_force_microscopy/JDbdsvdy">atomic force microscopy</a> (AFM), <a href="/facts/Transmission_electron_microscopy/Rpr8jpCn">transmission electron microscopy</a> (TEM) and <a href="/facts/F%25C3%25B6rster_resonance_energy_transfer/6dJTdAC6">Förster resonance energy transfer</a> (FRET). The constructed box was shown to have a unique reclosing mechanism, which enabled it to repeatedly open and close in response to a unique set of DNA or RNA keys. The authors proposed that this "DNA device can potentially be used for a broad range of applications such as controlling the function of single molecules, controlled drug delivery, and molecular computing."<a class="footnote-ref" id="fnref:105" href="#fn:105">105</a>
There are potential applications for DNA nanotechnology in nanomedicine, making use of its ability to perform computation in a <a href="/facts/Biocompatibility/UbGXs4XW">biocompatible</a> format to make "smart drugs" for <a href="/facts/Targeted_drug_delivery/cRjKKv6l">targeted drug delivery</a>, as well as for <a href="/facts/Diagnostic/VcsPwshd">diagnostic</a> applications. One such system being investigated uses a hollow DNA box containing proteins that induce <a href="/facts/Apoptosis/8dVzSm4y">apoptosis</a>, or cell death, that will only open when in proximity to a <a href="/facts/Cancer_cell/HXSQQjhw">cancer cell</a>.<a class="footnote-ref" id="fnref:106" href="#fn:106">106</a><a class="footnote-ref" id="fnref:107" href="#fn:107">107</a> There has additionally been interest in expressing these artificial structures in engineered living bacterial cells, most likely using the <a href="/facts/Transcription_(genetics)/bDgp9HDN">transcribed</a> RNA for the assembly, although it is unknown whether these complex structures are able to efficiently fold or assemble in the cell's <a href="/facts/Cytoplasm/Wa0sE6xf">cytoplasm</a>. If successful, this could enable <a href="/facts/Directed_evolution/DYJs0Hm2">directed evolution</a> of nucleic acid nanostructures.<a class="footnote-ref" id="fnref:108" href="#fn:108">108</a> 
Scientists at <a href="/facts/Oxford_University/ExVVQL6V">Oxford University</a> reported the self-assembly of four short strands of synthetic DNA into a cage which can enter cells and survive for at least 48 hours. The fluorescently labeled DNA <a href="/facts/Tetrahedron/7mkSrl6j">tetrahedra</a> were found to remain intact in the laboratory cultured human <a href="/facts/Kidney/HBspPrv9">kidney</a> cells despite the attack by cellular <a href="/facts/Enzymes/DSI1Fh7y">enzymes</a> after two days. This experiment showed the potential of drug delivery inside the living cells using the DNA ‘cage’.<a class="footnote-ref" id="fnref:109" href="#fn:109">109</a><a class="footnote-ref" id="fnref:110" href="#fn:110">110</a> A DNA <a href="/facts/Tetrahedron/7mkSrl6j">tetrahedron</a> was used to deliver <a href="/facts/RNA_Interference/oJRWBXJH">RNA Interference</a> (RNAi) in a mouse model, reported a team of researchers in <a href="/facts/MIT/Xk49vNA8">MIT</a>. Delivery of the interfering RNA for treatment has showed some success using <a href="/facts/Polymer/eF32E50K">polymer</a> or <a href="/facts/Lipid/TdQR87HX">lipid</a>, but there are limits of safety and imprecise targeting, in addition to short shelf life in the blood stream. The DNA nanostructure created by the team consists of six strands of DNA to form a tetrahedron, with one strand of RNA affixed to each of the six edges. The tetrahedron is further equipped with targeting protein, three <a href="/facts/Folate/8Eb7An6D">folate</a> molecules, which lead the DNA nanoparticles to the abundant <a href="/facts/Folate_receptor/tlH7q7cb">folate receptors</a> found on some tumors. The result showed that the gene expression targeted by the RNAi, <a href="/facts/Luciferase/7yl8dytU">luciferase</a>, dropped by more than half. This study shows promise in using DNA nanotechnology as an effective tool to deliver treatment using the emerging RNA Interference technology.<a class="footnote-ref" id="fnref:111" href="#fn:111">111</a><a class="footnote-ref" id="fnref:112" href="#fn:112">112</a> The DNA tetrahedron was also used in an effort to overcome the phenomena <a href="/facts/Multidrug_resistance/TPbHtAvz">multidrug resistance</a>. <a href="/facts/Doxorubicin/4XuC2ajQ">Doxorubicin</a> (DOX) was conjugated with the tetrahedron and was loaded into MCF-7 breast cancer cells that contained the <a href="/facts/P-glycoprotein/RY6qLKjq">P-glycoprotein</a> drug efflux pump. The results of the experiment showed the DOX was not being pumped out and apoptosis of the cancer cells was achieved. The tetrahedron without DOX was loaded into cells to test its biocompatibility, and the structure showed no cytotoxicity itself.<a class="footnote-ref" id="fnref:113" href="#fn:113">113</a> The DNA tetrahedron was also used as barcode for profiling the subcellular expression and distribution of proteins in cells for diagnostic purposes. The tetrahedral-nanostructured showed enhanced signal due to higher labeling efficiency and stability.<a class="footnote-ref" id="fnref:114" href="#fn:114">114</a>
Applications for DNA nanotechnology in nanomedicine also focus on mimicking the structure and function of naturally occurring <a href="/facts/Membrane_proteins/FboTeM8Q">membrane proteins</a> with designed DNA nanostructures. In 2012, Langecker et al.<a class="footnote-ref" id="fnref:115" href="#fn:115">115</a> introduced a pore-shaped DNA origami structure that can self-insert into <a href="/facts/Lipid_bilayer/CQKQ9l3g">lipid membranes</a> via hydrophobic <a href="/facts/Cholesterol/TnfPlYc1">cholesterol</a> modifications and induce ionic currents across the membrane. This first demonstration of a synthetic DNA ion channel was followed by a variety of pore-inducing designs ranging from a single <a href="/facts/Nucleic_acid_double_helix/XHeMuslB">DNA duplex</a>,<a class="footnote-ref" id="fnref:116" href="#fn:116">116</a> to small tile-based structures,<a class="footnote-ref" id="fnref:117" href="#fn:117">117</a><a class="footnote-ref" id="fnref:118" href="#fn:118">118</a><a class="footnote-ref" id="fnref:119" href="#fn:119">119</a><a class="footnote-ref" id="fnref:120" href="#fn:120">120</a><a class="footnote-ref" id="fnref:121" href="#fn:121">121</a> and large DNA origami transmembrane <a href="/facts/Porin_(protein)/XeNDIN4x">porins</a>.<a class="footnote-ref" id="fnref:122" href="#fn:122">122</a> Similar to naturally occurring protein <a href="/facts/Ion_channels/A46LARFH">ion channels</a>, this ensemble of synthetic DNA-made counterparts thereby spans multiple orders of magnitude in conductance. The study of the membrane-inserting single <a href="/facts/Nucleic_acid_double_helix/XHeMuslB">DNA duplex</a> showed that current must also flow on the DNA-lipid interface as no central channel lumen is present in the design that lets ions pass across the <a href="/facts/Lipid_bilayer/CQKQ9l3g">lipid bilayer</a>. This indicated that the DNA-induced lipid pore has a <a href="/facts/Torus/J9SkaV1W">toroidal</a> shape, rather than cylindrical, as <a href="/facts/Lipid/TdQR87HX">lipid headgroups</a> reorient to face towards the membrane-inserted part of the DNA.<a class="footnote-ref" id="fnref:123" href="#fn:123">123</a> Researchers from the <a href="/facts/University_of_Cambridge/0la7aX9o">University of Cambridge</a> and the <a href="/facts/University_of_Illinois_at_Urbana-Champaign/gusw7HoS">University of Illinois at Urbana-Champaign</a> then demonstrated that such a DNA-induced toroidal pore can facilitate rapid <a href="/facts/Scramblase/yC4r8hxr">lipid flip-flop</a> between the lipid bilayer leaflets. Utilizing this effect, they designed a synthetic DNA-built <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a> that flips lipids in biological membranes orders of magnitudes faster than naturally occurring proteins called <a href="/facts/Scramblases/yC4r8hxr">scramblases</a>.<a class="footnote-ref" id="fnref:124" href="#fn:124">124</a> This development highlights the potential of synthetic DNA nanostructures for personalized drugs and therapeutics.

<h2 id="design">Design</h2>
DNA nanostructures must be <a href="/facts/Rational_design/NbpkpmJJ">rationally designed</a> so that individual nucleic acid strands will assemble into the desired structures. This process usually begins with specification of a desired <a href="/facts/Nucleic_acid_tertiary_structure/ExXJ3V0x">target structure</a> or function. Then, the overall <a href="/facts/Nucleic_acid_secondary_structure/qXjlsFK5">secondary structure</a> of the target complex is determined, specifying the arrangement of nucleic acid strands within the structure, and which portions of those strands should be bound to each other. The last step is the <a href="/facts/Nucleic_acid_primary_structure/qAf4kTee">primary structure</a> design, which is the specification of the actual base sequences of each nucleic acid strand.<a class="footnote-ref" id="fnref:125" href="#fn:125">125</a><a class="footnote-ref" id="fnref:126" href="#fn:126">126</a>

<h3>Structural design</h3>
The first step in designing a nucleic acid nanostructure is to decide how a given structure should be represented by a specific arrangement of nucleic acid strands. This design step determines the secondary structure, or the positions of the base pairs that hold the individual strands together in the desired shape.<a class="footnote-ref" id="fnref:127" href="#fn:127">127</a> Several approaches have been demonstrated:

<ul><li>Tile-based structures. This approach breaks the target structure into smaller units with strong binding between the strands contained in each unit, and weaker interactions between the units. It is often used to make periodic lattices, but can also be used to implement algorithmic self-assembly, making them a platform for <a href="/facts/DNA_computing/NfNV4obg">DNA computing</a>. This was the dominant design strategy used from the mid-1990s until the mid-2000s, when the DNA origami methodology was developed.<a class="footnote-ref" id="fnref:128" href="#fn:128">128</a><a class="footnote-ref" id="fnref:129" href="#fn:129">129</a></li>
<li>Folding structures. An alternative to the tile-based approach, folding approaches make the nanostructure from one long strand, which can either have a designed sequence that folds due to its interactions with itself, or it can be folded into the desired shape by using shorter, "staple" strands. This latter method is called <a href="/facts/DNA_origami/Ce9Cv85M">DNA origami</a>, which allows forming nanoscale two- and three-dimensional shapes (see Discrete structures above).<a class="footnote-ref" id="fnref:130" href="#fn:130">130</a><a class="footnote-ref" id="fnref:131" href="#fn:131">131</a></li>
<li>Dynamic assembly. This approach directly controls the <a href="/facts/Chemical_kinetics/D8vyX4aE">kinetics</a> of DNA self-assembly, specifying all of the <a href="/facts/Reaction_intermediate/BjD9HAHt">intermediate</a> steps in the <a href="/facts/Reaction_mechanism/9lanyVuy">reaction mechanism</a> in addition to the final product. This is done using starting materials which adopt a <a href="/facts/Stem-loop/GysAzrX5">hairpin</a> structure; these then assemble into the final conformation in a <a href="/facts/Biochemical_cascade/bCsA7XXG">cascade</a> reaction, in a specific order (see Strand displacement cascades below). This approach has the advantage of proceeding <a href="/facts/Isothermal_process/6mkdESuS">isothermally</a>, at a constant temperature. This is in contrast to the thermodynamic approaches, which require a thermal <a href="/facts/Annealing_(biology)/4YD7V7hM">annealing</a> step where a temperature change is required to trigger the assembly and favor proper formation of the desired structure.<a class="footnote-ref" id="fnref:132" href="#fn:132">132</a><a class="footnote-ref" id="fnref:133" href="#fn:133">133</a></li></ul>
<h3>Sequence design</h3>
Main article: <a href="/facts/Nucleic_acid_design/WC4DFGNQ">Nucleic acid design</a>
After any of the above approaches are used to design the secondary structure of a target complex, an actual sequence of nucleotides that will form into the desired structure must be devised. Nucleic acid design is the process of assigning a specific nucleic acid base sequence to each of a structure's constituent strands so that they will associate into a desired conformation. Most methods have the goal of designing sequences so that the target structure has the lowest <a href="/facts/Gibbs_free_energy/bvenDuzh">energy</a>, and is thus the most thermodynamically favorable, while incorrectly assembled structures have higher energies and are thus disfavored. This is done either through simple, faster <a href="/facts/Heuristic/BcBEkv9c">heuristic</a> methods such as sequence symmetry minimization, or by using a full <a href="/facts/Nearest-neighbor_thermodynamic_parameters/4YD7V7hM">nearest-neighbor</a> thermodynamic model, which is more accurate but slower and more computationally intensive. Geometric models are used to examine <a href="/facts/Nucleic_acid_tertiary_structure/ExXJ3V0x">tertiary structure</a> of the nanostructures and to ensure that the complexes are not overly <a href="/facts/Strain_(chemistry)/xhAQQMH8">strained</a>.<a class="footnote-ref" id="fnref:134" href="#fn:134">134</a><a class="footnote-ref" id="fnref:135" href="#fn:135">135</a>
Nucleic acid design has similar goals to <a href="/facts/Protein_design/jFKIHFvc">protein design</a>. In both, the sequence of monomers is designed to favor the desired target structure and to disfavor other structures. Nucleic acid design has the advantage of being much computationally easier than protein design, because the simple base pairing rules are sufficient to predict a structure's energetic favorability, and detailed information about the overall three-dimensional folding of the structure is not required. This allows the use of simple heuristic methods that yield experimentally robust designs. Nucleic acid structures are less versatile than proteins in their function because of proteins' increased ability to fold into complex structures, and the limited chemical diversity of the four <a href="/facts/Nucleotide/pQKEwlJI">nucleotides</a> as compared to the twenty <a href="/facts/Proteinogenic_amino_acid/xoXITvm0">proteinogenic amino acids</a>.<a class="footnote-ref" id="fnref:136" href="#fn:136">136</a>

<h2 id="materials-and-methods">Materials and methods</h2>

The sequences of the DNA strands making up a target structure are designed computationally, using <a href="/facts/Molecular_modeling/bc80hbtm">molecular modeling</a> and <a href="/facts/Nucleic_acid_thermodynamics/4YD7V7hM">thermodynamic modeling</a> software.<a class="footnote-ref" id="fnref:137" href="#fn:137">137</a><a class="footnote-ref" id="fnref:138" href="#fn:138">138</a> The nucleic acids themselves are then synthesized using standard <a href="/facts/Oligonucleotide_synthesis/5q6Ncwfr">oligonucleotide synthesis</a> methods, usually automated in an <a href="/facts/Oligonucleotide_synthesis/5q6Ncwfr">oligonucleotide synthesizer</a>, and strands of custom sequences are commercially available.<a class="footnote-ref" id="fnref:139" href="#fn:139">139</a> Strands can be purified by <a href="/facts/Denaturation_(biochemistry)/Eh1hc3Bg">denaturing</a> <a href="/facts/Gel_electrophoresis/9J3GrsFk">gel electrophoresis</a> if needed,<a class="footnote-ref" id="fnref:140" href="#fn:140">140</a> and precise concentrations determined via any of several <a href="/facts/Nucleic_acid_quantitation/rI51qco1">nucleic acid quantitation</a> methods using <a href="/facts/Ultraviolet%25E2%2580%2593visible_spectroscopy/MUPLLczo">ultraviolet absorbance spectroscopy</a>.<a class="footnote-ref" id="fnref:141" href="#fn:141">141</a>
The fully formed target structures can be verified using <a href="/facts/Native_state/uBHLassN">native</a> gel electrophoresis, which gives size and shape information for the nucleic acid complexes. An <a href="/facts/Electrophoretic_mobility_shift_assay/I5w7VjJn">electrophoretic mobility shift assay</a> can assess whether a structure incorporates all desired strands.<a class="footnote-ref" id="fnref:142" href="#fn:142">142</a> <a href="/facts/Fluorescent_labeling/vKVEgb0v">Fluorescent labeling</a> and <a href="/facts/F%25C3%25B6rster_resonance_energy_transfer/6dJTdAC6">Förster resonance energy transfer</a> (FRET) are sometimes used to characterize the structure of the complexes.<a class="footnote-ref" id="fnref:143" href="#fn:143">143</a>
Nucleic acid structures can be directly imaged by <a href="/facts/Atomic_force_microscopy/JDbdsvdy">atomic force microscopy</a>, which is well suited to extended two-dimensional structures, but less useful for discrete three-dimensional structures because of the microscope tip's interaction with the fragile nucleic acid structure; <a href="/facts/Transmission_electron_microscopy/Rpr8jpCn">transmission electron microscopy</a> and <a href="/facts/Cryo-electron_microscopy/Hjbcf4Ec">cryo-electron microscopy</a> are often used in this case. Extended three-dimensional lattices are analyzed by <a href="/facts/X-ray_crystallography/WtI0F5DN">X-ray crystallography</a>.<a class="footnote-ref" id="fnref:144" href="#fn:144">144</a><a class="footnote-ref" id="fnref:145" href="#fn:145">145</a>

<h2 id="see-also">See also</h2>
<ul>
<li>Science portal</li><li>Technology portal</li></ul>
<ul><li><a href="/facts/International_Society_for_Nanoscale_Science%2c_Computation%2c_and_Engineering/T1Rgpzyy">International Society for Nanoscale Science, Computation, and Engineering</a></li>
<li><a href="/facts/Comparison_of_nucleic_acid_simulation_software/rn3ftAtp">Comparison of nucleic acid simulation software</a></li>
<li><a href="/facts/Molecular_models_of_DNA/lHWGKyal">Molecular models of DNA</a></li>
<li><a href="/facts/Nanobiotechnology/ScXT4ygG">Nanobiotechnology</a></li></ul>

<h2 id="further-reading">Further reading</h2>
General:

<ul><li>Seeman NC (June 2004). "Nanotechnology and the double helix". Scientific American. 290 (6): 64–75. <a href="/facts/Bibcode_(identifier)/9HtdQSGB">Bibcode</a>:<a href="https://ui.adsabs.harvard.edu/abs/2004SciAm.290f..64S">2004SciAm.290f..64S</a>. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1038%2Fscientificamerican0604-64">10.1038/scientificamerican0604-64</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/15195395">15195395</a>.—An article written for laypeople by the founder of the field</li>
<li>Seeman NC (June 2010). <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229">"Structural DNA nanotechnology: growing along with Nano Letters"</a>. Nano Letters. 10 (6): 1971–1978. <a href="/facts/Bibcode_(identifier)/9HtdQSGB">Bibcode</a>:<a href="https://ui.adsabs.harvard.edu/abs/2010NanoL..10.1971S">2010NanoL..10.1971S</a>. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1021%2Fnl101262u">10.1021/nl101262u</a>. <a href="/facts/PMC_(identifier)/dX1zMt71">PMC</a> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229">2901229</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/20486672">20486672</a>.—A review of results in the period 2001–2010</li>
<li>Seeman NC (2010). <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582">"Nanomaterials based on DNA"</a>. Annual Review of Biochemistry. 79: 65–87. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1146%2Fannurev-biochem-060308-102244">10.1146/annurev-biochem-060308-102244</a>. <a href="/facts/PMC_(identifier)/dX1zMt71">PMC</a> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582">3454582</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/20222824">20222824</a>.—A more comprehensive review including both old and new results in the field</li>
<li>Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. <a href="/facts/Bibcode_(identifier)/9HtdQSGB">Bibcode</a>:<a href="https://ui.adsabs.harvard.edu/abs/2011Sci...332.1140S">2011Sci...332.1140S</a>. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1126%2Fscience.332.6034.1140">10.1126/science.332.6034.1140</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/21636754">21636754</a>. and Service RF (June 2011). "DNA nanotechnology. Next step: DNA robots?". Science. 332 (6034): 1142. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1126%2Fscience.332.6034.1142">10.1126/science.332.6034.1142</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/21636755">21636755</a>..—A news article focusing on the history of the field and development of new applications</li>
<li>Zadegan RM, Norton ML (June 2012). <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397516">"Structural DNA nanotechnology: from design to applications"</a>. International Journal of Molecular Sciences. 13 (6): 7149–7162. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.3390%2Fijms13067149">10.3390/ijms13067149</a>. <a href="/facts/PMC_(identifier)/dX1zMt71">PMC</a> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397516">3397516</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/22837684">22837684</a>.—A very recent and comprehensive review in the field</li></ul>

Specific subfields:

<ul><li>Bath J, Turberfield AJ (May 2007). "DNA nanomachines". Nature Nanotechnology. 2 (5): 275–284. <a href="/facts/Bibcode_(identifier)/9HtdQSGB">Bibcode</a>:<a href="https://ui.adsabs.harvard.edu/abs/2007NatNa...2..275B">2007NatNa...2..275B</a>. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1038%2Fnnano.2007.104">10.1038/nnano.2007.104</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/18654284">18654284</a>.—A review of nucleic acid nanomechanical devices</li>
<li>Feldkamp U, Niemeyer CM (March 2006). "Rational design of DNA nanoarchitectures". Angewandte Chemie. 45 (12): 1856–1876. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1002%2Fanie.200502358">10.1002/anie.200502358</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/16470892">16470892</a>.—A review coming from the viewpoint of secondary structure design</li>
<li>Lin C, Liu Y, Rinker S, Yan H (August 2006). "DNA tile based self-assembly: building complex nanoarchitectures". ChemPhysChem. 7 (8): 1641–1647. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1002%2Fcphc.200600260">10.1002/cphc.200600260</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/16832805">16832805</a>.—A minireview specifically focusing on tile-based assembly</li>
<li>Zhang DY, Seelig G (February 2011). "Dynamic DNA nanotechnology using strand-displacement reactions". Nature Chemistry. 3 (2): 103–113. <a href="/facts/Bibcode_(identifier)/9HtdQSGB">Bibcode</a>:<a href="https://ui.adsabs.harvard.edu/abs/2011NatCh...3..103Z">2011NatCh...3..103Z</a>. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1038%2Fnchem.957">10.1038/nchem.957</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/21258382">21258382</a>.—A review of DNA systems making use of strand displacement mechanisms</li></ul>

<h2 id="external-links">External links</h2>

Wikimedia Commons has media related to DNA nanotechnology.

<ul><li><a href="https://www.youtube.com/watch?v=ITtGJUGXFKc">What is Bionanotechnology?</a>—a video introduction to DNA nanotechnology</li></ul>

<h2 id="references">References</h2>

<ol>
<li id="fn:1">History: Pelesko JA (2007). Self-assembly: the science of things that put themselves together. New York: Chapman & Hall/CRC. pp. 201, 242, 259. ISBN 978-1-58488-687-7. <a href="978-1-58488-687-7" target="_blank">978-1-58488-687-7</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></li>
<li id="fn:2">Overview: Seeman NC (June 2004). "Nanotechnology and the double helix". Scientific American. 290 (6): 64–75. Bibcode:2004SciAm.290f..64S. doi:10.1038/scientificamerican0604-64. PMID 15195395. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></li>
<li id="fn:3">History: See "Current crystallization protocol". Nadrian Seeman Lab. for a statement of the problem, and "DNA cages containing oriented guests". Nadrian Seeman Laboratory. for the proposed solution. <a href="http://seemanlab4.chem.nyu.edu/nano-pro.html" target="_blank">http://seemanlab4.chem.nyu.edu/nano-pro.html</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></li>
<li id="fn:4">Overview: Seeman NC (2010). "Nanomaterials based on DNA". Annual Review of Biochemistry. 79: 65–87. doi:10.1146/annurev-biochem-060308-102244. PMC 3454582. PMID 20222824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></li>
<li id="fn:5">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></li>
<li id="fn:6">Overview: Seeman NC (June 2004). "Nanotechnology and the double helix". Scientific American. 290 (6): 64–75. Bibcode:2004SciAm.290f..64S. doi:10.1038/scientificamerican0604-64. PMID 15195395. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></li>
<li id="fn:7">History: Pelesko JA (2007). Self-assembly: the science of things that put themselves together. New York: Chapman & Hall/CRC. pp. 201, 242, 259. ISBN 978-1-58488-687-7. <a href="978-1-58488-687-7" target="_blank">978-1-58488-687-7</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></li>
<li id="fn:8">DNA origami: Rothemund PW (2006). "Scaffolded DNA origami: from generalized multicrossovers to polygonal networks". In Chen J, Jonoska N, Rozenberg G (eds.). Nanotechnology: science and computation. Natural Computing Series. New York: Springer. pp. 3–21. CiteSeerX 10.1.1.144.1380. doi:10.1007/3-540-30296-4_1. ISBN 978-3-540-30295-7. <a href="978-3-540-30295-7" target="_blank">978-3-540-30295-7</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></li>
<li id="fn:9">History: Pelesko JA (2007). Self-assembly: the science of things that put themselves together. New York: Chapman & Hall/CRC. pp. 201, 242, 259. ISBN 978-1-58488-687-7. <a href="978-1-58488-687-7" target="_blank">978-1-58488-687-7</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></li>
<li id="fn:10">History/applications: Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></li>
<li id="fn:11">Yurke, Bernard; Turberfield, Andrew J.; Mills, Allen P.; Simmel, Friedrich C.; Neumann, Jennifer L. (August 2000). "A DNA-fuelled molecular machine made of DNA". Nature. 406 (6796): 605–608. Bibcode:2000Natur.406..605Y. doi:10.1038/35020524. ISSN 1476-4687. S2CID 2064216. <a href="https://www.nature.com/articles/35020524" target="_blank">https://www.nature.com/articles/35020524</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></li>
<li id="fn:12">DNA machines: Bath J, Turberfield AJ (May 2007). "DNA nanomachines". Nature Nanotechnology. 2 (5): 275–284. Bibcode:2007NatNa...2..275B. doi:10.1038/nnano.2007.104. PMID 18654284. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></li>
<li id="fn:13">Nanoarchitecture: Robinson BH, Seeman NC (August 1987). "The design of a biochip: a self-assembling molecular-scale memory device". Protein Engineering. 1 (4): 295–300. doi:10.1093/protein/1.4.295. PMID 3508280. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></li>
<li id="fn:14">Nanoarchitecture: Xiao S, Liu F, Rosen AE, Hainfeld JF, Seeman NC, Musier-Forsyth K, Kiehl RA (August 2002). "Selfassembly of metallic nanoparticle arrays by DNA scaffolding". Journal of Nanoparticle Research. 4 (4): 313–317. Bibcode:2002JNR.....4..313X. doi:10.1023/A:1021145208328. S2CID 2257083. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></li>
<li id="fn:15">DNA origami: Rothemund PW (2006). "Scaffolded DNA origami: from generalized multicrossovers to polygonal networks". In Chen J, Jonoska N, Rozenberg G (eds.). Nanotechnology: science and computation. Natural Computing Series. New York: Springer. pp. 3–21. CiteSeerX 10.1.1.144.1380. doi:10.1007/3-540-30296-4_1. ISBN 978-3-540-30295-7. <a href="978-3-540-30295-7" target="_blank">978-3-540-30295-7</a> <a href="#fnref:15" class="footnote-back-ref">↩</a></li>
<li id="fn:16">DNA origami: Rothemund PW (March 2006). "Folding DNA to create nanoscale shapes and patterns" (PDF). Nature. 440 (7082): 297–302. Bibcode:2006Natur.440..297R. doi:10.1038/nature04586. PMID 16541064. S2CID 4316391. <a href="https://rothemundlab.caltech.edu/downloads/folding_dna.pdf" target="_blank">https://rothemundlab.caltech.edu/downloads/folding_dna.pdf</a> <a href="#fnref:16" class="footnote-back-ref">↩</a></li>
<li id="fn:17">DNA origami: Douglas SM, Dietz H, Liedl T, Högberg B, Graf F, Shih WM (May 2009). "Self-assembly of DNA into nanoscale three-dimensional shapes". Nature. 459 (7245): 414–418. Bibcode:2009Natur.459..414D. doi:10.1038/nature08016. PMC 2688462. PMID 19458720. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688462" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688462</a> <a href="#fnref:17" class="footnote-back-ref">↩</a></li>
<li id="fn:18">History/applications: Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:18" class="footnote-back-ref">↩</a></li>
<li id="fn:19">Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:19" class="footnote-back-ref">↩</a></li>
<li id="fn:20">History/applications: Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:20" class="footnote-back-ref">↩</a></li>
<li id="fn:21">History: Hopkin K (August 2011). "Profile: 3-D seer". The Scientist. Archived from the original on 10 October 2011. Retrieved 8 August 2011. <a href="https://web.archive.org/web/20111010085248/http://the-scientist.com/2011/08/01/3-d-seer/" target="_blank">https://web.archive.org/web/20111010085248/http://the-scientist.com/2011/08/01/3-d-seer/</a> <a href="#fnref:21" class="footnote-back-ref">↩</a></li>
<li id="fn:22">History: Seeman NC (June 2010). "Structural DNA nanotechnology: growing along with Nano Letters". Nano Letters. 10 (6): 1971–1978. Bibcode:2010NanoL..10.1971S. doi:10.1021/nl101262u. PMC 2901229. PMID 20486672. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229</a> <a href="#fnref:22" class="footnote-back-ref">↩</a></li>
<li id="fn:23">Background: Pelesko JA (2007). Self-assembly: the science of things that put themselves together. New York: Chapman & Hall/CRC. pp. 5, 7. ISBN 978-1-58488-687-7. <a href="978-1-58488-687-7" target="_blank">978-1-58488-687-7</a> <a href="#fnref:23" class="footnote-back-ref">↩</a></li>
<li id="fn:24">Overview: Seeman NC (2010). "Nanomaterials based on DNA". Annual Review of Biochemistry. 79: 65–87. doi:10.1146/annurev-biochem-060308-102244. PMC 3454582. PMID 20222824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582</a> <a href="#fnref:24" class="footnote-back-ref">↩</a></li>
<li id="fn:25">Overview: Seeman NC (2010). "Nanomaterials based on DNA". Annual Review of Biochemistry. 79: 65–87. doi:10.1146/annurev-biochem-060308-102244. PMC 3454582. PMID 20222824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582</a> <a href="#fnref:25" class="footnote-back-ref">↩</a></li>
<li id="fn:26">Background: Long EC (1996). "Fundamentals of nucleic acids". In Hecht SM (ed.). Bioorganic chemistry: nucleic acids. New York: Oxford University Press. pp. 4–10. ISBN 978-0-19-508467-2. <a href="978-0-19-508467-2" target="_blank">978-0-19-508467-2</a> <a href="#fnref:26" class="footnote-back-ref">↩</a></li>
<li id="fn:27">Overview: Seeman NC (June 2004). "Nanotechnology and the double helix". Scientific American. 290 (6): 64–75. Bibcode:2004SciAm.290f..64S. doi:10.1038/scientificamerican0604-64. PMID 15195395. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:27" class="footnote-back-ref">↩</a></li>
<li id="fn:28">Overview: Seeman NC (2010). "Nanomaterials based on DNA". Annual Review of Biochemistry. 79: 65–87. doi:10.1146/annurev-biochem-060308-102244. PMC 3454582. PMID 20222824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582</a> <a href="#fnref:28" class="footnote-back-ref">↩</a></li>
<li id="fn:29">RNA nanotechnology: Chworos A, Severcan I, Koyfman AY, Weinkam P, Oroudjev E, Hansma HG, Jaeger L (December 2004). "Building programmable jigsaw puzzles with RNA". Science. 306 (5704): 2068–2072. Bibcode:2004Sci...306.2068C. doi:10.1126/science.1104686. PMID 15604402. S2CID 9296608. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:29" class="footnote-back-ref">↩</a></li>
<li id="fn:30">RNA nanotechnology: Guo P (December 2010). "The emerging field of RNA nanotechnology". Nature Nanotechnology. 5 (12): 833–842. Bibcode:2010NatNa...5..833G. doi:10.1038/nnano.2010.231. PMC 3149862. PMID 21102465. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149862" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149862</a> <a href="#fnref:30" class="footnote-back-ref">↩</a></li>
<li id="fn:31">Dynamic DNA nanotechnology: Zhang DY, Seelig G (February 2011). "Dynamic DNA nanotechnology using strand-displacement reactions". Nature Chemistry. 3 (2): 103–113. Bibcode:2011NatCh...3..103Z. doi:10.1038/nchem.957. PMID 21258382. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:31" class="footnote-back-ref">↩</a></li>
<li id="fn:32">Structural DNA nanotechnology: Seeman NC (November 2007). "An overview of structural DNA nanotechnology". Molecular Biotechnology. 37 (3): 246–257. doi:10.1007/s12033-007-0059-4. PMC 3479651. PMID 17952671. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651</a> <a href="#fnref:32" class="footnote-back-ref">↩</a></li>
<li id="fn:33">Overview: Seeman NC (June 2004). "Nanotechnology and the double helix". Scientific American. 290 (6): 64–75. Bibcode:2004SciAm.290f..64S. doi:10.1038/scientificamerican0604-64. PMID 15195395. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:33" class="footnote-back-ref">↩</a></li>
<li id="fn:34">Overview: Seeman NC (2010). "Nanomaterials based on DNA". Annual Review of Biochemistry. 79: 65–87. doi:10.1146/annurev-biochem-060308-102244. PMC 3454582. PMID 20222824. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454582</a> <a href="#fnref:34" class="footnote-back-ref">↩</a></li>
<li id="fn:35">Dynamic DNA nanotechnology: Zhang DY, Seelig G (February 2011). "Dynamic DNA nanotechnology using strand-displacement reactions". Nature Chemistry. 3 (2): 103–113. Bibcode:2011NatCh...3..103Z. doi:10.1038/nchem.957. PMID 21258382. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:35" class="footnote-back-ref">↩</a></li>
<li id="fn:36">Dynamic DNA nanotechnology: Lu Y, Liu J (December 2006). "Functional DNA nanotechnology: emerging applications of DNAzymes and aptamers". Current Opinion in Biotechnology. 17 (6): 580–588. doi:10.1016/j.copbio.2006.10.004. PMID 17056247. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:36" class="footnote-back-ref">↩</a></li>
<li id="fn:37">Simulation of DNA structures: Doye JP, Ouldridge TE, Louis AA, Romano F, Šulc P, Matek C, et al. (December 2013). "Coarse-graining DNA for simulations of DNA nanotechnology". Physical Chemistry Chemical Physics. 15 (47): 20395–20414. arXiv:1308.3843. Bibcode:2013PCCP...1520395D. doi:10.1039/C3CP53545B. PMID 24121860. S2CID 15324396. <a href="/wiki/ArXiv_(identifier)" target="_blank">/wiki/ArXiv_(identifier)</a> <a href="#fnref:37" class="footnote-back-ref">↩</a></li>
<li id="fn:38">Structural DNA nanotechnology: Seeman NC (November 2007). "An overview of structural DNA nanotechnology". Molecular Biotechnology. 37 (3): 246–257. doi:10.1007/s12033-007-0059-4. PMC 3479651. PMID 17952671. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651</a> <a href="#fnref:38" class="footnote-back-ref">↩</a></li>
<li id="fn:39">Structural DNA nanotechnology: Seeman NC (November 2007). "An overview of structural DNA nanotechnology". Molecular Biotechnology. 37 (3): 246–257. doi:10.1007/s12033-007-0059-4. PMC 3479651. PMID 17952671. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651</a> <a href="#fnref:39" class="footnote-back-ref">↩</a></li>
<li id="fn:40">DX arrays: Winfree E, Liu F, Wenzler LA, Seeman NC (August 1998). "Design and self-assembly of two-dimensional DNA crystals". Nature. 394 (6693): 539–544. Bibcode:1998Natur.394..539W. doi:10.1038/28998. PMID 9707114. S2CID 4385579. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:40" class="footnote-back-ref">↩</a></li>
<li id="fn:41">DX arrays: Liu F, Sha R, Seeman NC (10 February 1999). "Modifying the surface features of two-dimensional DNA crystals". Journal of the American Chemical Society. 121 (5): 917–922. doi:10.1021/ja982824a. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:41" class="footnote-back-ref">↩</a></li>
<li id="fn:42">Other arrays: Mao C, Sun W, Seeman NC (16 June 1999). "Designed two-dimensional DNA Holliday junction arrays visualized by atomic force microscopy". Journal of the American Chemical Society. 121 (23): 5437–5443. doi:10.1021/ja9900398. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:42" class="footnote-back-ref">↩</a></li>
<li id="fn:43">Other arrays: Constantinou PE, Wang T, Kopatsch J, Israel LB, Zhang X, Ding B, et al. (September 2006). "Double cohesion in structural DNA nanotechnology". Organic & Biomolecular Chemistry. 4 (18): 3414–3419. doi:10.1039/b605212f. PMC 3491902. PMID 17036134. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491902" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3491902</a> <a href="#fnref:43" class="footnote-back-ref">↩</a></li>
<li id="fn:44">Other arrays: Mathieu F, Liao S, Kopatsch J, Wang T, Mao C, Seeman NC (April 2005). "Six-helix bundles designed from DNA". Nano Letters. 5 (4): 661–665. Bibcode:2005NanoL...5..661M. doi:10.1021/nl050084f. PMC 3464188. PMID 15826105. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464188" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464188</a> <a href="#fnref:44" class="footnote-back-ref">↩</a></li>
<li id="fn:45">History: Seeman NC (June 2010). "Structural DNA nanotechnology: growing along with Nano Letters". Nano Letters. 10 (6): 1971–1978. Bibcode:2010NanoL..10.1971S. doi:10.1021/nl101262u. PMC 2901229. PMID 20486672. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229</a> <a href="#fnref:45" class="footnote-back-ref">↩</a></li>
<li id="fn:46">Algorithmic self-assembly: Rothemund PW, Papadakis N, Winfree E (December 2004). "Algorithmic self-assembly of DNA Sierpinski triangles". PLOS Biology. 2 (12): e424. doi:10.1371/journal.pbio.0020424. PMC 534809. PMID 15583715. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534809" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534809</a> <a href="#fnref:46" class="footnote-back-ref">↩</a></li>
<li id="fn:47">Algorithmic self-assembly: Barish RD, Rothemund PW, Winfree E (December 2005). "Two computational primitives for algorithmic self-assembly: copying and counting". Nano Letters. 5 (12): 2586–2592. Bibcode:2005NanoL...5.2586B. CiteSeerX 10.1.1.155.676. doi:10.1021/nl052038l. PMID 16351220. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:47" class="footnote-back-ref">↩</a></li>
<li id="fn:48">Design: Feldkamp U, Niemeyer CM (March 2006). "Rational design of DNA nanoarchitectures". Angewandte Chemie. 45 (12): 1856–1876. doi:10.1002/anie.200502358. PMID 16470892. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:48" class="footnote-back-ref">↩</a></li>
<li id="fn:49">DNA nanotubes: Rothemund PW, Ekani-Nkodo A, Papadakis N, Kumar A, Fygenson DK, Winfree E (December 2004). "Design and characterization of programmable DNA nanotubes". Journal of the American Chemical Society. 126 (50): 16344–16352. doi:10.1021/ja044319l. PMID 15600335. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20110309-104202496" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20110309-104202496</a> <a href="#fnref:49" class="footnote-back-ref">↩</a></li>
<li id="fn:50">DNA nanotubes: Yin P, Hariadi RF, Sahu S, Choi HM, Park SH, Labean TH, Reif JH (August 2008). "Programming DNA tube circumferences". Science. 321 (5890): 824–826. Bibcode:2008Sci...321..824Y. doi:10.1126/science.1157312. PMID 18687961. S2CID 12100380. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20111020-104503059" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20111020-104503059</a> <a href="#fnref:50" class="footnote-back-ref">↩</a></li>
<li id="fn:51">History: Seeman NC (June 2010). "Structural DNA nanotechnology: growing along with Nano Letters". Nano Letters. 10 (6): 1971–1978. Bibcode:2010NanoL..10.1971S. doi:10.1021/nl101262u. PMC 2901229. PMID 20486672. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901229</a> <a href="#fnref:51" class="footnote-back-ref">↩</a></li>
<li id="fn:52">Three-dimensional arrays: Zheng J, Birktoft JJ, Chen Y, Wang T, Sha R, Constantinou PE, et al. (September 2009). "From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal". Nature. 461 (7260): 74–77. Bibcode:2009Natur.461...74Z. doi:10.1038/nature08274. PMC 2764300. PMID 19727196. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764300" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764300</a> <a href="#fnref:52" class="footnote-back-ref">↩</a></li>
<li id="fn:53">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:53" class="footnote-back-ref">↩</a></li>
<li id="fn:54">DNA polyhedra: Zhang Y, Seeman NC (1 March 1994). "Construction of a DNA-truncated octahedron". Journal of the American Chemical Society. 116 (5): 1661–1669. doi:10.1021/ja00084a006. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:54" class="footnote-back-ref">↩</a></li>
<li id="fn:55">DNA polyhedra: Shih WM, Quispe JD, Joyce GF (February 2004). "A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron". Nature. 427 (6975): 618–621. Bibcode:2004Natur.427..618S. doi:10.1038/nature02307. PMID 14961116. S2CID 4419579. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:55" class="footnote-back-ref">↩</a></li>
<li id="fn:56">DNA polyhedra: Goodman RP, Schaap IA, Tardin CF, Erben CM, Berry RM, Schmidt CF, Turberfield AJ (December 2005). "Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication". Science. 310 (5754): 1661–1665. Bibcode:2005Sci...310.1661G. doi:10.1126/science.1120367. PMID 16339440. S2CID 13678773. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:56" class="footnote-back-ref">↩</a></li>
<li id="fn:57">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:57" class="footnote-back-ref">↩</a></li>
<li id="fn:58">DNA origami: Rothemund PW (March 2006). "Folding DNA to create nanoscale shapes and patterns" (PDF). Nature. 440 (7082): 297–302. Bibcode:2006Natur.440..297R. doi:10.1038/nature04586. PMID 16541064. S2CID 4316391. <a href="https://rothemundlab.caltech.edu/downloads/folding_dna.pdf" target="_blank">https://rothemundlab.caltech.edu/downloads/folding_dna.pdf</a> <a href="#fnref:58" class="footnote-back-ref">↩</a></li>
<li id="fn:59">Tikhomirov G, Petersen P, Qian L (December 2017). "Fractal assembly of micrometre-scale DNA origami arrays with arbitrary patterns". Nature. 552 (7683): 67–71. Bibcode:2017Natur.552...67T. doi:10.1038/nature24655. PMID 29219965. S2CID 4455780. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20171004-131452371" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20171004-131452371</a> <a href="#fnref:59" class="footnote-back-ref">↩</a></li>
<li id="fn:60">DNA origami: Douglas SM, Dietz H, Liedl T, Högberg B, Graf F, Shih WM (May 2009). "Self-assembly of DNA into nanoscale three-dimensional shapes". Nature. 459 (7245): 414–418. Bibcode:2009Natur.459..414D. doi:10.1038/nature08016. PMC 2688462. PMID 19458720. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688462" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2688462</a> <a href="#fnref:60" class="footnote-back-ref">↩</a></li>
<li id="fn:61">DNA boxes: Andersen ES, Dong M, Nielsen MM, Jahn K, Subramani R, Mamdouh W, et al. (May 2009). "Self-assembly of a nanoscale DNA box with a controllable lid". Nature. 459 (7243): 73–76. Bibcode:2009Natur.459...73A. doi:10.1038/nature07971. hdl:11858/00-001M-0000-0010-9363-9. PMID 19424153. S2CID 4430815. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:61" class="footnote-back-ref">↩</a></li>
<li id="fn:62">DNA boxes: Ke Y, Sharma J, Liu M, Jahn K, Liu Y, Yan H (June 2009). "Scaffolded DNA origami of a DNA tetrahedron molecular container". Nano Letters. 9 (6): 2445–2447. Bibcode:2009NanoL...9.2445K. doi:10.1021/nl901165f. PMID 19419184. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:62" class="footnote-back-ref">↩</a></li>
<li id="fn:63">Zaborova, O. V.; Voinova, A. D.; Shmykov, B. D.; Sergeyev, V. G. (2021). "Solid Lipid Nanoparticles for the Nucleic Acid Encapsulation". Reviews and Advances in Chemistry. 11 (3–4): 178–188. doi:10.1134/S2079978021030055. ISSN 2634-8276. S2CID 246946068. <a href="https://link.springer.com/10.1134/S2079978021030055" target="_blank">https://link.springer.com/10.1134/S2079978021030055</a> <a href="#fnref:63" class="footnote-back-ref">↩</a></li>
<li id="fn:64">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:64" class="footnote-back-ref">↩</a></li>
<li id="fn:65">Overview: Endo M, Sugiyama H (October 2009). "Chemical approaches to DNA nanotechnology". ChemBioChem. 10 (15): 2420–2443. doi:10.1002/cbic.200900286. PMID 19714700. S2CID 205554125. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:65" class="footnote-back-ref">↩</a></li>
<li id="fn:66">Nanoarchitecture: Zheng J, Constantinou PE, Micheel C, Alivisatos AP, Kiehl RA, Seeman NC (July 2006). "Two-dimensional nanoparticle arrays show the organizational power of robust DNA motifs". Nano Letters. 6 (7): 1502–1504. Bibcode:2006NanoL...6.1502Z. doi:10.1021/nl060994c. PMC 3465979. PMID 16834438. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465979" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465979</a> <a href="#fnref:66" class="footnote-back-ref">↩</a></li>
<li id="fn:67">Nanoarchitecture: Park SH, Pistol C, Ahn SJ, Reif JH, Lebeck AR, Dwyer C, LaBean TH (January 2006). "Finite-size, fully addressable DNA tile lattices formed by hierarchical assembly procedures". Angewandte Chemie. 45 (5): 735–739. Bibcode:2006AngCh.118.6759P. doi:10.1002/ange.200690141. PMID 16374784. <a href="https://doi.org/10.1002%2Fange.200690141" target="_blank">https://doi.org/10.1002%2Fange.200690141</a> <a href="#fnref:67" class="footnote-back-ref">↩</a></li>
<li id="fn:68">Nanoarchitecture: Cohen JD, Sadowski JP, Dervan PB (22 October 2007). "Addressing single molecules on DNA nanostructures". Angewandte Chemie. 46 (42): 7956–7959. doi:10.1002/anie.200702767. PMID 17763481. <a href="https://authors.library.caltech.edu/66743/" target="_blank">https://authors.library.caltech.edu/66743/</a> <a href="#fnref:68" class="footnote-back-ref">↩</a></li>
<li id="fn:69">Nanoarchitecture: Maune HT, Han SP, Barish RD, Bockrath M, Goddard WA, Rothemund PW, Winfree E (January 2010). "Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates". Nature Nanotechnology. 5 (1): 61–66. Bibcode:2010NatNa...5...61M. doi:10.1038/nnano.2009.311. PMID 19898497. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20100305-145736806" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20100305-145736806</a> <a href="#fnref:69" class="footnote-back-ref">↩</a></li>
<li id="fn:70">Nanoarchitecture: Liu J, Geng Y, Pound E, Gyawali S, Ashton JR, Hickey J, et al. (March 2011). "Metallization of branched DNA origami for nanoelectronic circuit fabrication". ACS Nano. 5 (3): 2240–2247. doi:10.1021/nn1035075. PMID 21323323. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:70" class="footnote-back-ref">↩</a></li>
<li id="fn:71">Nanoarchitecture: Deng Z, Mao C (August 2004). "Molecular lithography with DNA nanostructures". Angewandte Chemie. 43 (31): 4068–4070. doi:10.1002/anie.200460257. PMID 15300697. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:71" class="footnote-back-ref">↩</a></li>
<li id="fn:72">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:72" class="footnote-back-ref">↩</a></li>
<li id="fn:73">DNA machines: Bath J, Turberfield AJ (May 2007). "DNA nanomachines". Nature Nanotechnology. 2 (5): 275–284. Bibcode:2007NatNa...2..275B. doi:10.1038/nnano.2007.104. PMID 18654284. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:73" class="footnote-back-ref">↩</a></li>
<li id="fn:74">Dynamic DNA nanotechnology: Zhang DY, Seelig G (February 2011). "Dynamic DNA nanotechnology using strand-displacement reactions". Nature Chemistry. 3 (2): 103–113. Bibcode:2011NatCh...3..103Z. doi:10.1038/nchem.957. PMID 21258382. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:74" class="footnote-back-ref">↩</a></li>
<li id="fn:75">DNA machines: Bath J, Turberfield AJ (May 2007). "DNA nanomachines". Nature Nanotechnology. 2 (5): 275–284. Bibcode:2007NatNa...2..275B. doi:10.1038/nnano.2007.104. PMID 18654284. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:75" class="footnote-back-ref">↩</a></li>
<li id="fn:76">DNA machines: Mao C, Sun W, Shen Z, Seeman NC (January 1999). "A nanomechanical device based on the B-Z transition of DNA". Nature. 397 (6715): 144–146. Bibcode:1999Natur.397..144M. doi:10.1038/16437. PMID 9923675. S2CID 4406177. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:76" class="footnote-back-ref">↩</a></li>
<li id="fn:77">DNA machines: Yurke B, Turberfield AJ, Mills AP, Simmel FC, Neumann JL (August 2000). "A DNA-fuelled molecular machine made of DNA". Nature. 406 (6796): 605–608. Bibcode:2000Natur.406..605Y. doi:10.1038/35020524. PMID 10949296. S2CID 2064216. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:77" class="footnote-back-ref">↩</a></li>
<li id="fn:78">DNA machines: Yan H, Zhang X, Shen Z, Seeman NC (January 2002). "A robust DNA mechanical device controlled by hybridization topology". Nature. 415 (6867): 62–65. Bibcode:2002Natur.415...62Y. doi:10.1038/415062a. PMID 11780115. S2CID 52801697. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:78" class="footnote-back-ref">↩</a></li>
<li id="fn:79">DNA machines: Feng L, Park SH, Reif JH, Yan H (September 2003). "A two-state DNA lattice switched by DNA nanoactuator". Angewandte Chemie. 42 (36): 4342–4346. Bibcode:2003AngCh.115.4478F. doi:10.1002/ange.200351818. PMID 14502706. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:79" class="footnote-back-ref">↩</a></li>
<li id="fn:80">DNA boxes: Andersen ES, Dong M, Nielsen MM, Jahn K, Subramani R, Mamdouh W, et al. (May 2009). "Self-assembly of a nanoscale DNA box with a controllable lid". Nature. 459 (7243): 73–76. Bibcode:2009Natur.459...73A. doi:10.1038/nature07971. hdl:11858/00-001M-0000-0010-9363-9. PMID 19424153. S2CID 4430815. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:80" class="footnote-back-ref">↩</a></li>
<li id="fn:81">DNA machines: Goodman RP, Heilemann M, Doose S, Erben CM, Kapanidis AN, Turberfield AJ (February 2008). "Reconfigurable, braced, three-dimensional DNA nanostructures". Nature Nanotechnology. 3 (2): 93–96. Bibcode:2008NatNa...3...93G. doi:10.1038/nnano.2008.3. PMID 18654468. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:81" class="footnote-back-ref">↩</a></li>
<li id="fn:82">Applications: Douglas SM, Bachelet I, Church GM (February 2012). "A logic-gated nanorobot for targeted transport of molecular payloads". Science. 335 (6070): 831–834. Bibcode:2012Sci...335..831D. doi:10.1126/science.1214081. PMID 22344439. S2CID 9866509. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:82" class="footnote-back-ref">↩</a></li>
<li id="fn:83">Centola, Mathias; Poppleton, Erik; Ray, Sujay; Centola, Martin; Welty, Robb; Valero, Julián; Walter, Nils G.; Šulc, Petr; Famulok, Michael (2023-10-19). "A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower". Nature Nanotechnology: 1–11. doi:10.1038/s41565-023-01516-x. ISSN 1748-3395. PMC 10873200. PMID 37857824. <a href="https://www.nature.com/articles/s41565-023-01516-x" target="_blank">https://www.nature.com/articles/s41565-023-01516-x</a> <a href="#fnref:83" class="footnote-back-ref">↩</a></li>
<li id="fn:84">DNA machines: Bath J, Turberfield AJ (May 2007). "DNA nanomachines". Nature Nanotechnology. 2 (5): 275–284. Bibcode:2007NatNa...2..275B. doi:10.1038/nnano.2007.104. PMID 18654284. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:84" class="footnote-back-ref">↩</a></li>
<li id="fn:85">DNA walkers: Shin JS, Pierce NA (September 2004). "A synthetic DNA walker for molecular transport". Journal of the American Chemical Society. 126 (35): 10834–10835. doi:10.1021/ja047543j. PMID 15339155. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20170224-152823096" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20170224-152823096</a> <a href="#fnref:85" class="footnote-back-ref">↩</a></li>
<li id="fn:86">DNA walkers: Sherman WB, Seeman NC (July 2004). "A precisely controlled DNA biped walking device". Nano Letters. 4 (7): 1203–1207. Bibcode:2004NanoL...4.1203S. doi:10.1021/nl049527q. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:86" class="footnote-back-ref">↩</a></li>
<li id="fn:87">DNA walkers: Škugor M, Valero J, Murayama K, Centola M, Asanuma H, Famulok M (May 2019). "Orthogonally Photocontrolled Non-Autonomous DNA Walker". Angewandte Chemie. 58 (21): 6948–6951. doi:10.1002/anie.201901272. PMID 30897257. S2CID 85446523. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:87" class="footnote-back-ref">↩</a></li>
<li id="fn:88">DNA walkers: Tian Y, He Y, Chen Y, Yin P, Mao C (July 2005). "A DNAzyme that walks processively and autonomously along a one-dimensional track". Angewandte Chemie. 44 (28): 4355–4358. Bibcode:2005AngCh.117.4429T. doi:10.1002/ange.200500703. PMID 15945114. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:88" class="footnote-back-ref">↩</a></li>
<li id="fn:89">DNA walkers: Bath J, Green SJ, Turberfield AJ (July 2005). "A free-running DNA motor powered by a nicking enzyme". Angewandte Chemie. 44 (28): 4358–4361. doi:10.1002/anie.200501262. PMID 15959864. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:89" class="footnote-back-ref">↩</a></li>
<li id="fn:90">Functional DNA walkers: Lund K, Manzo AJ, Dabby N, Michelotti N, Johnson-Buck A, Nangreave J, et al. (May 2010). "Molecular robots guided by prescriptive landscapes". Nature. 465 (7295): 206–210. Bibcode:2010Natur.465..206L. doi:10.1038/nature09012. PMC 2907518. PMID 20463735. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907518" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907518</a> <a href="#fnref:90" class="footnote-back-ref">↩</a></li>
<li id="fn:91">Functional DNA walkers: Valero J, Pal N, Dhakal S, Walter NG, Famulok M (June 2018). "A bio-hybrid DNA rotor-stator nanoengine that moves along predefined tracks". Nature Nanotechnology. 13 (6): 496–503. Bibcode:2018NatNa..13..496V. doi:10.1038/s41565-018-0109-z. PMC 5994166. PMID 29632399. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994166" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994166</a> <a href="#fnref:91" class="footnote-back-ref">↩</a></li>
<li id="fn:92">Functional DNA walkers: He Y, Liu DR (November 2010). "Autonomous multistep organic synthesis in a single isothermal solution mediated by a DNA walker". Nature Nanotechnology. 5 (11): 778–782. Bibcode:2010NatNa...5..778H. doi:10.1038/nnano.2010.190. PMC 2974042. PMID 20935654. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974042" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974042</a> <a href="#fnref:92" class="footnote-back-ref">↩</a></li>
<li id="fn:93">Pan J, Li F, Cha TG, Chen H, Choi JH (August 2015). "Recent progress on DNA based walkers". Current Opinion in Biotechnology. 34: 56–64. doi:10.1016/j.copbio.2014.11.017. PMID 25498478. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:93" class="footnote-back-ref">↩</a></li>
<li id="fn:94">Dynamic DNA nanotechnology: Zhang DY, Seelig G (February 2011). "Dynamic DNA nanotechnology using strand-displacement reactions". Nature Chemistry. 3 (2): 103–113. Bibcode:2011NatCh...3..103Z. doi:10.1038/nchem.957. PMID 21258382. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:94" class="footnote-back-ref">↩</a></li>
<li id="fn:95">Kinetic assembly: Yin P, Choi HM, Calvert CR, Pierce NA (January 2008). "Programming biomolecular self-assembly pathways". Nature. 451 (7176): 318–322. Bibcode:2008Natur.451..318Y. doi:10.1038/nature06451. PMID 18202654. S2CID 4354536. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507</a> <a href="#fnref:95" class="footnote-back-ref">↩</a></li>
<li id="fn:96">Fuzzy and Boolean logic gates based on DNA: Zadegan RM, Jepsen MD, Hildebrandt LL, Birkedal V, Kjems J (April 2015). "Construction of a fuzzy and Boolean logic gates based on DNA". Small. 11 (15): 1811–1817. doi:10.1002/smll.201402755. PMID 25565140. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:96" class="footnote-back-ref">↩</a></li>
<li id="fn:97">Strand displacement cascades: Seelig G, Soloveichik D, Zhang DY, Winfree E (December 2006). "Enzyme-free nucleic acid logic circuits". Science. 314 (5805): 1585–1588. Bibcode:2006Sci...314.1585S. doi:10.1126/science.1132493. PMID 17158324. S2CID 10966324. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20110309-104200565" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20110309-104200565</a> <a href="#fnref:97" class="footnote-back-ref">↩</a></li>
<li id="fn:98">Strand displacement cascades: Qian L, Winfree E (June 2011). "Scaling up digital circuit computation with DNA strand displacement cascades". Science. 332 (6034): 1196–1201. Bibcode:2011Sci...332.1196Q. doi:10.1126/science.1200520. PMID 21636773. S2CID 10053541. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:98" class="footnote-back-ref">↩</a></li>
<li id="fn:99">Kinetic assembly: Yin P, Choi HM, Calvert CR, Pierce NA (January 2008). "Programming biomolecular self-assembly pathways". Nature. 451 (7176): 318–322. Bibcode:2008Natur.451..318Y. doi:10.1038/nature06451. PMID 18202654. S2CID 4354536. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507</a> <a href="#fnref:99" class="footnote-back-ref">↩</a></li>
<li id="fn:100">Structural DNA nanotechnology: Seeman NC (November 2007). "An overview of structural DNA nanotechnology". Molecular Biotechnology. 37 (3): 246–257. doi:10.1007/s12033-007-0059-4. PMC 3479651. PMID 17952671. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651</a> <a href="#fnref:100" class="footnote-back-ref">↩</a></li>
<li id="fn:101">History/applications: Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:101" class="footnote-back-ref">↩</a></li>
<li id="fn:102">Structural DNA nanotechnology: Seeman NC (November 2007). "An overview of structural DNA nanotechnology". Molecular Biotechnology. 37 (3): 246–257. doi:10.1007/s12033-007-0059-4. PMC 3479651. PMID 17952671. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479651</a> <a href="#fnref:102" class="footnote-back-ref">↩</a></li>
<li id="fn:103">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:103" class="footnote-back-ref">↩</a></li>
<li id="fn:104">Applications: Rietman EA (2001). Molecular engineering of nanosystems. Springer. pp. 209–212. ISBN 978-0-387-98988-4. Retrieved 17 April 2011. <a href="978-0-387-98988-4" target="_blank">978-0-387-98988-4</a> <a href="#fnref:104" class="footnote-back-ref">↩</a></li>
<li id="fn:105">Zadegan RM, Jepsen MD, Thomsen KE, Okholm AH, Schaffert DH, Andersen ES, et al. (November 2012). "Construction of a 4 zeptoliters switchable 3D DNA box origami". ACS Nano. 6 (11): 10050–10053. doi:10.1021/nn303767b. PMID 23030709. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:105" class="footnote-back-ref">↩</a></li>
<li id="fn:106">History/applications: Service RF (June 2011). "DNA nanotechnology. DNA nanotechnology grows up". Science. 332 (6034): 1140–1, 1143. Bibcode:2011Sci...332.1140S. doi:10.1126/science.332.6034.1140. PMID 21636754. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:106" class="footnote-back-ref">↩</a></li>
<li id="fn:107">Applications: Jungmann R, Renner S, Simmel FC (April 2008). "From DNA nanotechnology to synthetic biology". HFSP Journal. 2 (2): 99–109. doi:10.2976/1.2896331. PMC 2645571. PMID 19404476. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645571" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2645571</a> <a href="#fnref:107" class="footnote-back-ref">↩</a></li>
<li id="fn:108">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:108" class="footnote-back-ref">↩</a></li>
<li id="fn:109">Lovy, Howard (5 July 2011). "DNA cages can unleash meds inside cells". fiercedrugdelivery.com. Archived from the original on September 27, 2013. Retrieved 22 September 2013. <a href="https://web.archive.org/web/20130927192141/http://www.fiercedrugdelivery.com/story/dna-cages-can-unleash-meds-inside-cells/2011-07-05" target="_blank">https://web.archive.org/web/20130927192141/http://www.fiercedrugdelivery.com/story/dna-cages-can-unleash-meds-inside-cells/2011-07-05</a> <a href="#fnref:109" class="footnote-back-ref">↩</a></li>
<li id="fn:110">Walsh AS, Yin H, Erben CM, Wood MJ, Turberfield AJ (July 2011). "DNA cage delivery to mammalian cells". ACS Nano. 5 (7): 5427–5432. doi:10.1021/nn2005574. PMID 21696187. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:110" class="footnote-back-ref">↩</a></li>
<li id="fn:111">Trafton, Anne (4 June 2012). "Researchers achieve RNA interference, in a lighter package". MIT News. Retrieved 22 September 2013. <a href="http://web.mit.edu/newsoffice/2012/rna-interference-lightweight-nanoparticle-0604.html" target="_blank">http://web.mit.edu/newsoffice/2012/rna-interference-lightweight-nanoparticle-0604.html</a> <a href="#fnref:111" class="footnote-back-ref">↩</a></li>
<li id="fn:112">Lee H, Lytton-Jean AK, Chen Y, Love KT, Park AI, Karagiannis ED, et al. (June 2012). "Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery". Nature Nanotechnology. 7 (6): 389–393. Bibcode:2012NatNa...7..389L. doi:10.1038/NNANO.2012.73. PMC 3898745. PMID 22659608. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898745" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898745</a> <a href="#fnref:112" class="footnote-back-ref">↩</a></li>
<li id="fn:113">Kim KR, Kim DR, Lee T, Yhee JY, Kim BS, Kwon IC, Ahn DR (March 2013). "Drug delivery by a self-assembled DNA tetrahedron for overcoming drug resistance in breast cancer cells". Chemical Communications. 49 (20): 2010–2012. doi:10.1039/c3cc38693g. PMID 23380739. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:113" class="footnote-back-ref">↩</a></li>
<li id="fn:114">Sundah NR, Ho NR, Lim GS, Natalia A, Ding X, Liu Y, et al. (September 2019). "Barcoded DNA nanostructures for the multiplexed profiling of subcellular protein distribution". Nature Biomedical Engineering. 3 (9): 684–694. doi:10.1038/s41551-019-0417-0. PMID 31285580. S2CID 195825879. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:114" class="footnote-back-ref">↩</a></li>
<li id="fn:115">DNA ion channels: Langecker M, Arnaut V, Martin TG, List J, Renner S, Mayer M, et al. (November 2012). "Synthetic lipid membrane channels formed by designed DNA nanostructures". Science. 338 (6109): 932–936. Bibcode:2012Sci...338..932L. doi:10.1126/science.1225624. PMC 3716461. PMID 23161995. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3716461" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3716461</a> <a href="#fnref:115" class="footnote-back-ref">↩</a></li>
<li id="fn:116">DNA ion channels: Göpfrich K, Li CY, Mames I, Bhamidimarri SP, Ricci M, Yoo J, et al. (July 2016). "Ion Channels Made from a Single Membrane-Spanning DNA Duplex". Nano Letters. 16 (7): 4665–4669. Bibcode:2016NanoL..16.4665G. doi:10.1021/acs.nanolett.6b02039. PMC 4948918. PMID 27324157. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948918" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948918</a> <a href="#fnref:116" class="footnote-back-ref">↩</a></li>
<li id="fn:117">DNA ion channels: Burns JR, Stulz E, Howorka S (June 2013). "Self-assembled DNA nanopores that span lipid bilayers". Nano Letters. 13 (6): 2351–2356. Bibcode:2013NanoL..13.2351B. CiteSeerX 10.1.1.659.7660. doi:10.1021/nl304147f. PMID 23611515. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:117" class="footnote-back-ref">↩</a></li>
<li id="fn:118">DNA ion channels: Burns JR, Göpfrich K, Wood JW, Thacker VV, Stulz E, Keyser UF, Howorka S (November 2013). "Lipid-bilayer-spanning DNA nanopores with a bifunctional porphyrin anchor". Angewandte Chemie. 52 (46): 12069–12072. doi:10.1002/anie.201305765. PMC 4016739. PMID 24014236. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016739" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016739</a> <a href="#fnref:118" class="footnote-back-ref">↩</a></li>
<li id="fn:119">DNA ion channels: Seifert A, Göpfrich K, Burns JR, Fertig N, Keyser UF, Howorka S (February 2015). "Bilayer-spanning DNA nanopores with voltage-switching between open and closed state". ACS Nano. 9 (2): 1117–1126. doi:10.1021/nn5039433. PMC 4508203. PMID 25338165. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508203" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508203</a> <a href="#fnref:119" class="footnote-back-ref">↩</a></li>
<li id="fn:120">DNA ion channels: Göpfrich K, Zettl T, Meijering AE, Hernández-Ainsa S, Kocabey S, Liedl T, Keyser UF (May 2015). "DNA-Tile Structures Induce Ionic Currents through Lipid Membranes". Nano Letters. 15 (5): 3134–3138. Bibcode:2015NanoL..15.3134G. doi:10.1021/acs.nanolett.5b00189. PMID 25816075. <a href="https://www.repository.cam.ac.uk/handle/1810/259995" target="_blank">https://www.repository.cam.ac.uk/handle/1810/259995</a> <a href="#fnref:120" class="footnote-back-ref">↩</a></li>
<li id="fn:121">DNA ion channels: Burns JR, Seifert A, Fertig N, Howorka S (February 2016). "A biomimetic DNA-based channel for the ligand-controlled transport of charged molecular cargo across a biological membrane". Nature Nanotechnology. 11 (2): 152–156. Bibcode:2016NatNa..11..152B. doi:10.1038/nnano.2015.279. PMID 26751170. <a href="https://discovery.ucl.ac.uk/id/eprint/1474103/" target="_blank">https://discovery.ucl.ac.uk/id/eprint/1474103/</a> <a href="#fnref:121" class="footnote-back-ref">↩</a></li>
<li id="fn:122">DNA ion channels: Göpfrich K, Li CY, Ricci M, Bhamidimarri SP, Yoo J, Gyenes B, et al. (September 2016). "Large-Conductance Transmembrane Porin Made from DNA Origami". ACS Nano. 10 (9): 8207–8214. doi:10.1021/acsnano.6b03759. PMC 5043419. PMID 27504755. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043419" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043419</a> <a href="#fnref:122" class="footnote-back-ref">↩</a></li>
<li id="fn:123">DNA ion channels: Göpfrich K, Li CY, Mames I, Bhamidimarri SP, Ricci M, Yoo J, et al. (July 2016). "Ion Channels Made from a Single Membrane-Spanning DNA Duplex". Nano Letters. 16 (7): 4665–4669. Bibcode:2016NanoL..16.4665G. doi:10.1021/acs.nanolett.6b02039. PMC 4948918. PMID 27324157. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948918" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948918</a> <a href="#fnref:123" class="footnote-back-ref">↩</a></li>
<li id="fn:124">DNA scramblase: Ohmann A, Li CY, Maffeo C, Al Nahas K, Baumann KN, Göpfrich K, et al. (June 2018). "A synthetic enzyme built from DNA flips 107 lipids per second in biological membranes". Nature Communications. 9 (1): 2426. Bibcode:2018NatCo...9.2426O. doi:10.1038/s41467-018-04821-5. PMC 6013447. PMID 29930243. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013447" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013447</a> <a href="#fnref:124" class="footnote-back-ref">↩</a></li>
<li id="fn:125">Design: Feldkamp U, Niemeyer CM (March 2006). "Rational design of DNA nanoarchitectures". Angewandte Chemie. 45 (12): 1856–1876. doi:10.1002/anie.200502358. PMID 16470892. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:125" class="footnote-back-ref">↩</a></li>
<li id="fn:126">Design: Brenneman A, Condon A (25 September 2002). "Strand design for biomolecular computation". Theoretical Computer Science. 287: 39–58. doi:10.1016/S0304-3975(02)00135-4. <a href="https://doi.org/10.1016%2FS0304-3975%2802%2900135-4" target="_blank">https://doi.org/10.1016%2FS0304-3975%2802%2900135-4</a> <a href="#fnref:126" class="footnote-back-ref">↩</a></li>
<li id="fn:127">Design: Feldkamp U, Niemeyer CM (March 2006). "Rational design of DNA nanoarchitectures". Angewandte Chemie. 45 (12): 1856–1876. doi:10.1002/anie.200502358. PMID 16470892. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:127" class="footnote-back-ref">↩</a></li>
<li id="fn:128">Design: Feldkamp U, Niemeyer CM (March 2006). "Rational design of DNA nanoarchitectures". Angewandte Chemie. 45 (12): 1856–1876. doi:10.1002/anie.200502358. PMID 16470892. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:128" class="footnote-back-ref">↩</a></li>
<li id="fn:129">Overview: Lin C, Liu Y, Rinker S, Yan H (August 2006). "DNA tile based self-assembly: building complex nanoarchitectures". ChemPhysChem. 7 (8): 1641–1647. doi:10.1002/cphc.200600260. PMID 16832805. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:129" class="footnote-back-ref">↩</a></li>
<li id="fn:130">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:130" class="footnote-back-ref">↩</a></li>
<li id="fn:131">DNA origami: Rothemund PW (March 2006). "Folding DNA to create nanoscale shapes and patterns" (PDF). Nature. 440 (7082): 297–302. Bibcode:2006Natur.440..297R. doi:10.1038/nature04586. PMID 16541064. S2CID 4316391. <a href="https://rothemundlab.caltech.edu/downloads/folding_dna.pdf" target="_blank">https://rothemundlab.caltech.edu/downloads/folding_dna.pdf</a> <a href="#fnref:131" class="footnote-back-ref">↩</a></li>
<li id="fn:132">Overview: Pinheiro AV, Han D, Shih WM, Yan H (November 2011). "Challenges and opportunities for structural DNA nanotechnology". Nature Nanotechnology. 6 (12): 763–772. Bibcode:2011NatNa...6..763P. doi:10.1038/nnano.2011.187. PMC 3334823. PMID 22056726. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334823</a> <a href="#fnref:132" class="footnote-back-ref">↩</a></li>
<li id="fn:133">Kinetic assembly: Yin P, Choi HM, Calvert CR, Pierce NA (January 2008). "Programming biomolecular self-assembly pathways". Nature. 451 (7176): 318–322. Bibcode:2008Natur.451..318Y. doi:10.1038/nature06451. PMID 18202654. S2CID 4354536. <a href="https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507" target="_blank">https://resolver.caltech.edu/CaltechAUTHORS:20170224-143742507</a> <a href="#fnref:133" class="footnote-back-ref">↩</a></li>
<li id="fn:134">Design: Brenneman A, Condon A (25 September 2002). "Strand design for biomolecular computation". Theoretical Computer Science. 287: 39–58. doi:10.1016/S0304-3975(02)00135-4. <a href="https://doi.org/10.1016%2FS0304-3975%2802%2900135-4" target="_blank">https://doi.org/10.1016%2FS0304-3975%2802%2900135-4</a> <a href="#fnref:134" class="footnote-back-ref">↩</a></li>
<li id="fn:135">Design: Dirks RM, Lin M, Winfree E, Pierce NA (15 February 2004). "Paradigms for computational nucleic acid design". Nucleic Acids Research. 32 (4): 1392–1403. doi:10.1093/nar/gkh291. PMC 390280. PMID 14990744. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280</a> <a href="#fnref:135" class="footnote-back-ref">↩</a></li>
<li id="fn:136">Design: Dirks RM, Lin M, Winfree E, Pierce NA (15 February 2004). "Paradigms for computational nucleic acid design". Nucleic Acids Research. 32 (4): 1392–1403. doi:10.1093/nar/gkh291. PMC 390280. PMID 14990744. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280</a> <a href="#fnref:136" class="footnote-back-ref">↩</a></li>
<li id="fn:137">Design: Brenneman A, Condon A (25 September 2002). "Strand design for biomolecular computation". Theoretical Computer Science. 287: 39–58. doi:10.1016/S0304-3975(02)00135-4. <a href="https://doi.org/10.1016%2FS0304-3975%2802%2900135-4" target="_blank">https://doi.org/10.1016%2FS0304-3975%2802%2900135-4</a> <a href="#fnref:137" class="footnote-back-ref">↩</a></li>
<li id="fn:138">Design: Dirks RM, Lin M, Winfree E, Pierce NA (15 February 2004). "Paradigms for computational nucleic acid design". Nucleic Acids Research. 32 (4): 1392–1403. doi:10.1093/nar/gkh291. PMC 390280. PMID 14990744. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC390280</a> <a href="#fnref:138" class="footnote-back-ref">↩</a></li>
<li id="fn:139">Methods: Ellington A, Pollard JD (1 May 2001). "Synthesis and Purification of Oligonucleotides". Current Protocols in Molecular Biology. 42: 2.11.1–2.11.25. doi:10.1002/0471142727.mb0211s42. ISBN 978-0471142720. PMID 18265179. S2CID 205152989. <a href="978-0471142720" target="_blank">978-0471142720</a> <a href="#fnref:139" class="footnote-back-ref">↩</a></li>
<li id="fn:140">Methods: Ellington A, Pollard JD (1 May 2001). "Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis". Current Protocols in Molecular Biology. 42: Unit2.12. doi:10.1002/0471142727.mb0212s42. ISBN 978-0471142720. PMID 18265180. S2CID 27187583. <a href="978-0471142720" target="_blank">978-0471142720</a> <a href="#fnref:140" class="footnote-back-ref">↩</a></li>
<li id="fn:141">Methods: Gallagher SR, Desjardins P (1 July 2011). "Quantitation of nucleic acids and proteins". Current Protocols Essential Laboratory Techniques. Vol. 5. doi:10.1002/9780470089941.et0202s5. ISBN 978-0470089934. S2CID 94329398. <a href="978-0470089934" target="_blank">978-0470089934</a> <a href="#fnref:141" class="footnote-back-ref">↩</a></li>
<li id="fn:142">Methods: Chory J, Pollard JD (1 May 2001). "Separation of Small DNA Fragments by Conventional Gel Electrophoresis". Current Protocols in Molecular Biology. 47: Unit2.7. doi:10.1002/0471142727.mb0207s47. ISBN 978-0471142720. PMID 18265187. S2CID 43406338. <a href="978-0471142720" target="_blank">978-0471142720</a> <a href="#fnref:142" class="footnote-back-ref">↩</a></li>
<li id="fn:143">Methods: Walter NG (1 February 2003). "Probing RNA Structural Dynamics and Function by Fluorescence Resonance Energy Transfer (FRET)". Current Protocols in Nucleic Acid Chemistry. 11: 11.10.1–11.10.23. doi:10.1002/0471142700.nc1110s11. ISBN 978-0471142706. PMID 18428904. S2CID 9978415. <a href="978-0471142706" target="_blank">978-0471142706</a> <a href="#fnref:143" class="footnote-back-ref">↩</a></li>
<li id="fn:144">Methods: Lin C, Ke Y, Chhabra R, Sharma J, Liu Y, Yan H (2011). "Synthesis and Characterization of Self-Assembled DNA Nanostructures". In Zuccheri G, Samorì B (eds.). DNA Nanotechnology. Methods in Molecular Biology. Vol. 749. pp. 1–11. doi:10.1007/978-1-61779-142-0_1. ISBN 978-1-61779-141-3. PMID 21674361. <a href="978-1-61779-141-3" target="_blank">978-1-61779-141-3</a> <a href="#fnref:144" class="footnote-back-ref">↩</a></li>
<li id="fn:145">Methods: Bloomfield VA, Crothers DM, Tinoco Jr I (2000). Nucleic acids: structures, properties, and functions. Sausalito, Calif: University Science Books. pp. 84–86, 396–407. ISBN 978-0-935702-49-1. <a href="978-0-935702-49-1" target="_blank">978-0-935702-49-1</a> <a href="#fnref:145" class="footnote-back-ref">↩</a></li>
</ol>

DNA nanotechnology open-in-new

DNA nanotechnology