Menu
Home
People
Places
Arts
History
Plants & Animals
Science
Life & Culture
Technology
Reference.org
Nucleic acid methods
open-in-new
<h2 id="purification">Purification</h2> <ul><li><a href="/facts/DNA_extraction/MblSSc76">DNA extraction</a><a class="footnote-ref" id="fnref:1" href="#fn:1"><sup>1</sup></a></li> <li><a href="/facts/Phenol%E2%80%93chloroform_extraction/LnyQz6fd">Phenol–chloroform extraction</a></li> <li><a href="/facts/Minicolumn_purification/rGdyD9h9">Minicolumn purification</a></li> <li><a href="/facts/RNA_extraction/vrm8w7mm">RNA extraction</a></li> <li><a href="/facts/Boom_method/5EIkXhly">Boom method</a></li> <li><a href="/facts/Synchronous_coefficient_of_drag_alteration/4oH331jH">Synchronous coefficient of drag alteration</a> (SCODA) DNA purification</li></ul> <h2 id="quantification">Quantification</h2> <ul><li>Abundance in weight: spectroscopic <a href="/facts/Nucleic_acid_quantitation/rI51qco1">nucleic acid quantitation</a></li> <li>Absolute abundance in number: <a href="/facts/Real-time_polymerase_chain_reaction/jYLeLlQM">real-time polymerase chain reaction</a> (quantitative PCR)</li> <li>High-throughput relative abundance: <a href="/facts/DNA_microarray/hofkMvou">DNA microarray</a></li> <li>High-throughput absolute abundance: <a href="/facts/Serial_analysis_of_gene_expression/zfdu5FY2">serial analysis of gene expression</a> (SAGE)</li> <li>Size: <a href="/facts/Gel_electrophoresis/9J3GrsFk">gel electrophoresis</a></li></ul> <h2 id="synthesis">Synthesis</h2> <ul><li><i>De novo</i>: <a href="/facts/Oligonucleotide_synthesis/5q6Ncwfr">oligonucleotide synthesis</a></li> <li>Amplification: <a href="/facts/Polymerase_chain_reaction/G0jWzJy1">polymerase chain reaction</a> (PCR), <a href="/facts/Loop-mediated_isothermal_amplification/DYlgSBl3">loop-mediated isothermal amplification</a> (LAMP), <a href="/facts/Transcription-mediated_amplification/Kd3cVYUT">transcription-mediated amplification</a> (TMA)</li></ul> <h2 id="kinetics">Kinetics</h2> <ul><li><a href="/facts/Multi-parametric_surface_plasmon_resonance/wkUkrLUT">Multi-parametric surface plasmon resonance</a><a class="footnote-ref" id="fnref:2" href="#fn:2"><sup>2</sup></a><a class="footnote-ref" id="fnref:3" href="#fn:3"><sup>3</sup></a></li> <li><a href="/facts/Dual-polarization_interferometry/opICunH9">Dual-polarization interferometry</a><a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a></li> <li><a href="/facts/Quartz_crystal_microbalance_with_dissipation_monitoring/6eINsv7W">Quartz crystal microbalance with dissipation monitoring</a> (QCM-D)<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a></li></ul> <h2 id="gene-function">Gene function</h2> <ul><li><a href="/facts/RNA_interference/oJRWBXJH">RNA interference</a><a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a></li></ul> <h2 id="other">Other</h2> <ul><li><a href="/facts/Bisulfite_sequencing/TAXg1DL7">Bisulfite sequencing</a></li> <li><a href="/facts/DNA_sequencing/hsPsNCQW">DNA sequencing</a></li> <li><a href="/facts/Expression_cloning/kRwnM9QW">Expression cloning</a></li> <li><a href="/facts/Fluorescence_in_situ_hybridization/L4juqPoq">Fluorescence in situ hybridization</a></li> <li><a href="/facts/Lab-on-a-chip/5ABHeIdC">Lab-on-a-chip</a></li> <li><a href="/facts/Comparison_of_nucleic_acid_simulation_software/rn3ftAtp">Comparison of nucleic acid simulation software</a></li> <li><a href="/facts/Northern_blot/pRUrdy2n">Northern blot</a></li> <li><a href="/facts/Nuclear_run-on/9s9f05gD">Nuclear run-on</a> assay</li> <li><a href="/facts/Radioactivity_in_the_life_sciences/guwcMDWP">Radioactivity in the life sciences</a></li> <li><a href="/facts/Southern_blot/HXrr0Gz3">Southern blot</a></li> <li><a href="/facts/Differential_centrifugation/h2EyfDk2">Differential centrifugation</a> (sucrose gradient)</li> <li><a href="/facts/Toeprinting_assay/6geQa9Al">Toeprinting assay</a></li> <li>Several <a href="/facts/Bioinformatics/D5x2L8ee">bioinformatics</a> methods, as seen in <a href="/facts/List_of_RNA_structure_prediction_software/sTHP2t3c">list of RNA structure prediction software</a></li></ul> <h2 id="see-also">See also</h2> <ul><li><i><a href="/facts/CSH_Protocols/GYA0U8Bh">CSH Protocols</a></i></li> <li><i><a href="/facts/Current_Protocols/jhdIzuml">Current Protocols</a></i></li></ul> <h2 id="external-links">External links</h2> <ul><li><a href="https://web.archive.org/web/20090822064448/http://www.genome.ou.edu/protocol_book/protocol_index.html">Protocols for Recombinant DNA Isolation, Cloning, and Sequencing</a></li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>https://journals.sagepub.com/doi/abs/10.1177/10815589251327503 <a href="https://journals.sagepub.com/doi/abs/10.1177/10815589251327503" target="_blank">https://journals.sagepub.com/doi/abs/10.1177/10815589251327503</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778. <a href="https://zenodo.org/record/889908" target="_blank">https://zenodo.org/record/889908</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2563918" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2563918</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281. <a href="https://www.nature.com/articles/418244a" target="_blank">https://www.nature.com/articles/418244a</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> </ol>