Menu
Home
People
Places
Arts
History
Plants & Animals
Science
Life & Culture
Technology
Reference.org
Magic angle spinning
open-in-new
<h2 id="variations">Variations</h2> <h3>High Resolution Magic-Angle Spinning (HR-MAS)</h3> <p>HRMAS is usually applied to solutions and gels where dipole-dipole interactions are insufficiently averaged by the intermediate molecular motion. HRMAS can dramatically average out residual dipolar interactions and result in spectra with linewidths similar to solution-state NMR. HRMAS links the gap between solution-state and solid-state NMR, and enable the use of solution-state experiments<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> </p><p>HRMAS and its medical research application was first described in a 1997 study of human brain tissues from a neurodegenerative disorder.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a> </p> <h3>Solution Magic Angle Spinning</h3> <p>Use of Magic Angle Spinning has been extended from solid-state to liquid (solution) NMR.<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> </p> <h3>Magic angle turning</h3> <p>The magic-angle-turning (MAT) technique introduced by Gan employs slow (approximately 30 Hz) rotation of a powdered sample at the magic angle, in concert with pulses synchronized to 1/3 of the rotor period, to obtain isotropic-shift information in one dimension of a 2D spectrum.<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a> </p> <h3>Magic angle spinning spheres</h3> <p>Rather than using cylindrical rotors, spinning spheres can be spun stably at the magic angle, which can be used to increase the filling factor of the coils, hence improve the sensitivity.<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a> Magic angle spinning spheres allow stable MAS with faster spinning rates.<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a> </p> <h2 id="applications">Applications</h2> <p>There are significant advantages to using MAS NMR in structural biology. Magic angle spinning can be used to characterize large insoluble systems, including biological assemblies and intact viruses, that cannot be studied with other methods.<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a> </p> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>E. R. Andrew; A. Bradbury; R. G. Eades (1958). "Nuclear magnetic resonance spectra from a crystal rotated at high speed". Nature. 182 (4650): 1659. Bibcode:1958Natur.182.1659A. doi:10.1038/1821659a0. <a href="https://doi.org/10.1038%2F1821659a0" target="_blank">https://doi.org/10.1038%2F1821659a0</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>I. J. Lowe (1959). "Free Induction Decays of Rotating Solids". Phys. Rev. Lett. 2 (7): 285–287. Bibcode:1959PhRvL...2..285L. doi:10.1103/PhysRevLett.2.285. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Jacek W. Hennel; Jacek Klinowski (2005). "Magic Angle Spinning: A Historical Perspective". In Jacek Klinowski (ed.). New techniques in solid-state NMR. Vol. 246. Springer. pp. 1–14. doi:10.1007/b98646. ISBN 978-3-540-22168-5. PMID 22160286. {{cite book}}: |journal= ignored (help) (New techniques in solid-state NMR, p. 1, at Google Books) <a href="978-3-540-22168-5" target="_blank">978-3-540-22168-5</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Händel, Heidi; Gesele, Elke; Gottschall, Klaus; Albert, Klaus (2003). "Application of HRMAS 1H NMR Spectroscopy To Investigate Interactions between Ligands and Synthetic Receptors". Angewandte Chemie International Edition. 42 (4): 438–442. doi:10.1002/anie.200390133. PMID 12569511. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Cheng, Leo; et., al. (1997). "Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy". Proc Natl Acad Sci U S A. 94 (12): 6408–13. Bibcode:1997PNAS...94.6408C. doi:10.1073/pnas.94.12.6408. PMC 21063. PMID 9177231. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC21063" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC21063</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Polenova, Tatyana; Gupta, Rupal; Goldbourt, Amir (20 Mar 2016). "Magic Angle Spinning NMR Spectroscopy: A Versatile Technique for Structural and Dynamic Analysis of Solid-Phase Systems". Analytical Chemistry. 87 (11): 5458–5469. doi:10.1021/ac504288u. PMC 4890703. PMID 25794311. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890703" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890703</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Hu, J.Z.; Wang, W.; Liu, F.; Solum, M.S.; Alderman, D.W.; Pugmire, R.J.; Grant, D.M. (1995). "Magic-Angle-Turning Experiments for Measuring Chemical-Shift-Tensor Principal Values in Powdered Solids". Journal of Magnetic Resonance, Series A. 113 (2): 210–222. Bibcode:1995JMagR.113..210H. doi:10.1006/jmra.1995.1082. <a href="https://linkinghub.elsevier.com/retrieve/pii/S1064185885710820" target="_blank">https://linkinghub.elsevier.com/retrieve/pii/S1064185885710820</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>Chen, Pinhui; Albert, Brice J.; Gao, Chukun; Alaniva, Nicholas; Price, Lauren E.; Scott, Faith J.; Saliba, Edward P.; Sesti, Erika L.; Judge, Patrick T.; Fisher, Edward W.; Barnes, Alexander B. (2018). "Magic angle spinning spheres". Science Advances. 4 (9): eaau1540. Bibcode:2018SciA....4.1540C. doi:10.1126/sciadv.aau1540. ISSN 2375-2548. PMC 6155130. PMID 30255153. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155130" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155130</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>Osborn Popp, Thomas M.; Däpp, Alexander; Gao, Chukun; Chen, Pin-Hui; Price, Lauren E.; Alaniva, Nicholas H.; Barnes, Alexander B. (2020-06-18). "Highly stable magic angle spinning spherical rotors". Magnetic Resonance. 1 (1): 97–103. doi:10.5194/mr-1-97-2020. hdl:20.500.11850/465781. ISSN 2699-0016. S2CID 221741694. <a href="https://mr.copernicus.org/articles/1/97/2020/" target="_blank">https://mr.copernicus.org/articles/1/97/2020/</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Porat-Dahlerbruch, Gal; Goldbourt, Amir; Polenova, Tatyana (29 September 2021). "Virus Structures and Dynamics by Magic-Angle Spinning NMR". Annual Review of Virology. 8 (1): 219–237. doi:10.1146/annurev-virology-011921-064653. ISSN 2327-056X. PMC 8973440. PMID 34586870. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973440" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973440</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> </ol>