Membrane models

<h2 id="gorter-and-grendels-membrane-theory-1925">Gorter and Grendel's membrane theory (1925)</h2>

Evert Gorter and François Grendel (Dutch physiologists) approached the discovery of our present model of the <a href="/facts/Plasma_membrane/vAXSD0DT">plasma membrane</a> structure as a <a href="/facts/Lipid_bilayer/CQKQ9l3g">lipid bi-layer</a>. They simply hypothesized that if the plasma membrane is a bi-layer, then the <a href="/facts/Surface_area/WNowzFCw">surface area</a> of the mono-layer of lipids measured would be double the surface area of the plasma membrane. To examine their hypothesis, they performed an experiment in which they extracted <a href="/facts/Lipids/TdQR87HX">lipids</a> from a known number of red blood cells (<a href="/facts/Red_blood_cell/Kggpq8Jh">erythrocytes</a>) of different mammalian sources, such as humans, goats, sheep, etc. and then spreading the lipids as a mono-layer in a <a href="/facts/Langmuir-Blodgett_trough/jFYvKQyh">Langmuir-Blodgett trough</a>. They measured the total surface area of the plasma membrane of red blood cells, and using Langmuir's method, they measured the area of the monolayer of lipids. In comparing the two, they calculated an estimated ratio of 2:1 Mono-layer of lipids: Plasma membrane. This supported their hypothesis, which led to the conclusion that cell membranes are composed of two opposing molecular layers.<a class="footnote-ref" id="fnref:1" href="#fn:1">1</a> The two scientists proposed a structure for this bi-layer, with the <a href="/facts/Chemical_polarity/KlIJ0zjB">polar</a> <a href="/facts/Hydrophilic/k8p95WYu">hydrophilic</a> heads facing outwards towards the aqueous environment and the <a href="/facts/Hydrophobic/Ifya0CXQ">hydrophobic</a> tails facing inwards away from the aqueous surroundings on both sides of the membrane. Although they arrived at the right conclusions, some of the experimental data were incorrect such as the miscalculation of the area and pressure of the lipid monolayer and the incompleteness of lipid extraction. They also failed to describe membrane function and had false assumptions such as that of plasma membranes consisting mostly of lipids. However, on the whole, this envisioning of the lipid bi-layer structure became the basic underlying assumption for each successive refinement in a modern understanding of membrane function.<a class="footnote-ref" id="fnref:2" href="#fn:2">2</a>

<h2 id="the-davson-and-danielli-model-with-backup-from-robertson-19401960">The Davson and Danielli model with backup from Robertson (1940–1960)</h2>
Following the proposal of Gorter and Grendel, doubts inevitably arose over the veracity of having just a simple lipid bi-layer as a membrane. For instance, their model could not provide answers to questions on surface tension, permeability, and the electric resistance of membranes. Therefore, physiologist <a href="/facts/Hugh_Davson/W69F2CW7">Hugh Davson</a> and biologist <a href="/facts/James_Danielli/V8sgWH7C">James Danielli</a> suggested that membranes indeed do have proteins. According to them, the existence of these "membrane proteins" explained that which couldn't be answered by the Gorter-Grendel model.
In 1935, Davson and Danielli proposed that biological membranes are made up of lipid bi-layers that are coated on both sides with thin sheets of protein and they simplified their model into the "pauci-molecular" theory.<a class="footnote-ref" id="fnref:3" href="#fn:3">3</a> This theory declared that all biological membranes have a "lipoid" center surrounded by mono-layers of lipid that are covered by protein mono-layers. In short, their model was illustrated as a "sandwich" of protein-lipid-protein. The Davson-Danielli model threw new light on the understanding of cell membranes, by stressing the important role played by proteins in biological membranes.
By the 1950s, cell biologists verified the existence of plasma membranes through the use of <a href="/facts/Electron_microscopy/XtaBktwF">electron microscopy</a> (which accounted for higher resolutions). J. David Robertson used this method to propose the unit membrane model.<a class="footnote-ref" id="fnref:4" href="#fn:4">4</a> Basically, he suggested that all cellular membranes share a similar underlying structure, the <a href="/facts/Unit_membrane/vAXSD0DT">unit membrane</a>. Using heavy metal staining, Robertson's proposal also seemed to agree instantaneously with the Davson-Danielli model. According to the trilaminar pattern of the cellular membrane viewed by Robertson, he suggested that the membranes consist of a lipid bi-layer covered on both surfaces with thin sheets of proteins(mucoprotiens). This suggestion was a great boost to the proposal of Davson and Danielli.<a class="footnote-ref" id="fnref:5" href="#fn:5">5</a> However, even with Robertson's substantiation, the Davson-Danielli model had serious complications, a major one being that the proteins studied were mainly globular and couldn't therefore fit into the model's claim of thin protein sheets. These difficulties with the model stimulated new research in membrane organization and paved the way for the fluid mosaic model, which was proposed in 1972.

<h2 id="singer-and-nicolsons-fluid-mosaic-model-1972">Singer and Nicolson's fluid mosaic model (1972)</h2>
Main article: <a href="/facts/Fluid_mosaic_model/mEEYz4av">Fluid mosaic model</a>
In 1972, <a href="/facts/Seymour_Jonathan_Singer/HTzTclTd">S. Jonathan Singer</a> and <a href="/facts/Garth_L._Nicolson/MlmMaohC">Garth Nicolson</a> developed new ideas for membrane structure. Their proposal was the <a href="/facts/Fluid_mosaic_model/mEEYz4av">fluid mosaic model</a>, which is one of the dominant models now. It has two key features—a mosaic of proteins embedded in the membrane, and the membrane being a fluid bi-layer of lipids. The lipid bi-layer suggestion agrees with previous models but views proteins as globular entities embedded in the layer instead of thin sheets on the surface.
According to the model, membrane proteins are in three classes based on how they are linked to the lipid bi-layer:

<ol><li><a href="/facts/Integral_protein/Vztpmlu1">Integral proteins</a>: Immersed in the bi-layer and held in place by the affinity of <a href="/facts/Hydrophobic/Ifya0CXQ">hydrophobic</a> parts of the protein for the hydrophobic tails of <a href="/facts/Phospholipids/u6GhNCF5">phospholipids</a> on interior of the layer.</li>
<li><a href="/facts/Peripheral_protein/EGsWYg2e">Peripheral proteins</a>: More <a href="/facts/Hydrophilic/k8p95WYu">hydrophilic</a>, and thus are non-<a href="/facts/Covalent/EzMLf3Pz">covalently</a> linked to the polar heads of phospholipids and other hydrophilic parts of other membrane proteins on the surface of the membrane.</li>
<li><a href="/facts/Lipid-anchored_protein/GaGD1pJL">Lipid anchored proteins</a>: Essentially hydrophilic, so, are also located on the surface of the membrane, and are covalently attached to lipid molecules embedded in the layer.</li></ol>
As for the fluid nature of the membrane, the lipid components are capable of moving parallel to the membrane surface and are in constant motion. Many proteins are also capable of that motion within the membrane. However, some are restricted in their mobility due to them being anchored to structural elements such as the <a href="/facts/Cytoskeleton/1sWlJRX9">cytoskeleton</a> on either side of the membrane.
In general, this model explains most of the criticisms of the <a href="/facts/Davson%E2%80%93Danielli_model/ReQSSfCU">Davson–Danielli model</a>. It eliminated the need to accommodate membrane proteins in thin surface layers, proposed that the variability in the protein/lipid ratios of different membranes simply means that different membranes vary in the amount of protein they contain, and showed how the exposure of lipid-head groups at the membrane surface is compatible with their sensitivity to <a href="/facts/Phospholipase/kWbLYcA5">phospholipase</a> digestion. Also, the fluidity of the lipid bi-layers and the intermingling of their components within the membrane make it easy to visualize the mobility of both lipids and proteins.

<h2 id="henderson-and-unwins-membrane-theory">Henderson and Unwin's membrane theory</h2>

Henderson and Unwin have studied the purple membrane by electron microscopy, using a method for determining the projected structures of unstained crystalline specimens. By applying the method to tilted specimens, and using the principles put forward by DeRosier and <a href="/facts/Aaron_Klug/5A2dp3qy">Klug</a> for the combination of such two-dimensional views, they obtained a 3-dimensional map of the membrane at 7 Å resolution. The map reveals the location of the protein and lipid components, the arrangement of the polypeptide chains within each protein molecule, and the relationship of the protein molecules in the lattice.<a class="footnote-ref" id="fnref:6" href="#fn:6">6</a>
High-resolution micrographs of crystalline arrays of membrane proteins, taken at a low dose of electrons to minimize radiation damage, have been exploited to determine the three-dimensional structure by a <a href="/facts/Fourier_transform/XXhKJtc8">Fourier transform</a>. 
Recent studies on negatively stained rat <a href="/facts/Hepatocyte/USpvDz1n">hepatocyte</a> Gap™ junctions subjected to 3-dimensional Fourier reconstructions (of low-dose <a href="/facts/Electron_micrograph/M6fk5VED">electron micrographs</a>) indicate that the six protein sub-units are arranged in a cylinder slightly tilted tangentially, enclosing a channel 2 nm wide at the extracellular region. The dimensions of the channel within the membrane were narrower but could not be resolved (Unwin and Zampighi, 1980). A small radical movement of the sub-units at the cytoplasmic ends could reduce the sub-unit inclination tangential to six-fold axis and close the channel.<a class="footnote-ref" id="fnref:7" href="#fn:7">7</a>
Further details of the molecular organization should emerge as more methods of preparation become available, so that high-resolution 3-dimensional images comparable to the purple membranes are obtained. By using ingenious procedures for the analysis of periodic arrays of biological <a href="/facts/Macromolecule/039ULSLg">macromolecules</a>, in which data from low-dose electron images and diffraction patterns were combined, Henderson and Unwin (1975) reconstructed a three-dimensional image of purple membranes at 0.7 nm resolution. Glucose embedding was employed to alleviate dehydration damage and low doses (< 0.5 e/A*) to reduce the irradiation damage. The electron micrographs of unstained membranes were recorded such that the only source of contrast was a weak phase contrast induced by defocusing.
In their experiment, Unwin and Henderson found that protein extends to both sides of the lipid bi-layer and is composed of seven α-helices packed about 1–1.2 nm apart, 3.5–4.0 nm in length, running perpendicular to the plane of membrane. The molecules are organized around a 3-fold axis with a 2 nm-wide space at the center that is filled with lipids. This elegant work represents the most significant step forward thus far, as it has for the first time provided us with the structure of an integral membrane protein in situ.
The availability of the amino acid sequence, together with information about the electron scattering density from the work of Henderson and Unwin, has stimulated model-building efforts (Engleman et al., 1980) to fit the <a href="/facts/Bacteriorhodopsin/nlfvmDPm">bacteriorhodopsin</a> sequence information into a series of α-helical segments.

<h2 id="kervin-and-overduins-proteolipid-code-2024">Kervin and Overduin's proteolipid code (2024)</h2>

Building on the fluid mosaic model, a framework called the proteolipid code was proposed in order to explain membrane organization.<a class="footnote-ref" id="fnref:8" href="#fn:8">8</a> The proteolipid code relies on the concept of a zone, which is a functional region of membrane that is assembled and stabilized with both protein and lipid dependency. Integral and lipid-anchored proteins are proposed to form three types of zones: proteins with an associated lipid fingerprint,<a class="footnote-ref" id="fnref:9" href="#fn:9">9</a> protein islands, and lipid-only voids. Although the latter do not contain proteins as part of their internal particle set or primary structure, they do contain proteins in their quaternary association with the former two zone types which influence the composition of the void. The idea that lipids can cluster independently of proteins through lipid-lipid interactions and then recruit integral proteins is forbidden in the framework, although lipid clustering is allowed and is designated as zone secondary structure. 

<h2 id="see-also">See also</h2>
<ul><li><a href="/facts/Cell_biology/kdIBGHub">Cell biology</a></li>
<li><a href="/facts/Cell_theory/71xtwL7f">Cell theory</a></li>
<li><a href="/facts/History_of_cell_membrane_theory/VYMC6cKH">History of cell membrane theory</a></li>
<li><a href="/facts/Membrane_protein/FboTeM8Q">Membrane protein</a></li></ul>

<h2 id="references">References</h2>

<ol>
<li id="fn:1">"Membrane – An Introduction" (PDF). Wiley-VCH. Retrieved 9 October 2015. <a href="http://www.wiley-vch.de/books/sample/3527404716_c01.pdf" target="_blank">http://www.wiley-vch.de/books/sample/3527404716_c01.pdf</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></li>
<li id="fn:2">Becker's World of the Cell (8th ed.). University of Wisconsin-Madison: Jeff Hardin. 2012. <a href="#fnref:2" class="footnote-back-ref">↩</a></li>
<li id="fn:3">Robertson, J. David (1981). "Membrane Structure" (PDF). The Journal of Cell Biology. 91 (3): 189s – 204s. doi:10.1083/jcb.91.3.189s. PMID 7033238. Retrieved 9 October 2015. <a href="http://jcb.rupress.org/content/91/3/189s.full.pdf" target="_blank">http://jcb.rupress.org/content/91/3/189s.full.pdf</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></li>
<li id="fn:4">Heuser, John E. "In Memory of J.David Robertson" (PDF). heuserlab.wustl.edu. Retrieved 8 October 2015. <a href="http://www.heuserlab.wustl.edu/experience/Robertson%20obit.pdf" target="_blank">http://www.heuserlab.wustl.edu/experience/Robertson%20obit.pdf</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></li>
<li id="fn:5">Hardin, Jeff; Kleinsmith, Lewis J.; Bertoni, Gregory; Becker, Wayne M. (2012). World of the Cell (Eighth ed.). US: Pearson Benjamin Cummings. pp. 158–163. <a href="#fnref:5" class="footnote-back-ref">↩</a></li>
<li id="fn:6">R. Henderson & P. N. T. Unwin (September 4, 1975). "Three-dimensional model of purple membrane obtained by electron microscopy". Nature. 257 (5521). Cambridge: MRC Laboratory of Molecular Biology: 28–32. Bibcode:1975Natur.257...28H. doi:10.1038/257028a0. PMID 1161000. <a href="/wiki/Bibcode_(identifier)" target="_blank">/wiki/Bibcode_(identifier)</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></li>
<li id="fn:7">Malhotra, S. K. (1983). The Plasma membrane. Canada: John Wiley & Sons. pp. 3, 92, 93, 95. <a href="#fnref:7" class="footnote-back-ref">↩</a></li>
<li id="fn:8">Kervin, Troy A.; Overduin, Michael (December 2024). "Membranes are functionalized by a proteolipid code". BMC Biology. 22 (1): 46. doi:10.1186/s12915-024-01849-6. ISSN 1741-7007. PMC 10898092. PMID 38414038. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898092" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898092</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></li>
<li id="fn:9">Corradi, Valentina; Mendez-Villuendas, Eduardo; Ingólfsson, Helgi I.; Gu, Ruo-Xu; Siuda, Iwona; Melo, Manuel N.; Moussatova, Anastassiia; DeGagné, Lucien J.; Sejdiu, Besian I.; Singh, Gurpreet; Wassenaar, Tsjerk A.; Delgado Magnero, Karelia; Marrink, Siewert J.; Tieleman, D. Peter (2018-06-27). "Lipid–Protein Interactions Are Unique Fingerprints for Membrane Proteins". ACS Central Science. 4 (6): 709–717. doi:10.1021/acscentsci.8b00143. ISSN 2374-7943. PMC 6028153. PMID 29974066. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028153" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028153</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></li>
</ol>

Membrane models open-in-new

Membrane models