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Yellow fluorescent protein
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<h2 id="evolution-of-yfp-from-gfp">Evolution of YFP from GFP</h2> <p>Four protein mutations from the wild-type GFP found in Aequorea Victoria jellyfish were needed to create the YFP mutant. The most important mutation was the replacement of threonine with tyrosine at residue position 203<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> (the substitution is denoted by T203Y, where T and Y represent the single letter code for the amino acids threonine and tyrosine, respectively). </p> <h2 id="see-also">See also</h2> <ul><li><a href="/facts/Red_fluorescent_protein/XXej1etn">Red fluorescent protein</a></li></ul> <h2 id="external-links">External links</h2> <ul><li><a href="http://www.microscopyu.com/articles/livecellimaging/fpintro.html">Introduction to fluorescent proteins</a></li> <li><a href="https://www.fpbase.org/protein/eyfp/">EYFP</a> on FPbase</li> <li>Miyawaki, Atsushi; Llopis, Juan; Heim, Roger; McCaffery, J. Michael; Adams, Joseph A.; Ikura, Mitsuhiko; Tsien, Roger Y. (1997). <a href="https://doi.org/10.1038%2F42264">"Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin"</a>. <i>Nature</i>. 388 (6645): 882–7. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1038%2F42264">10.1038/42264</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/9278050">9278050</a>.</li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Ormö, Mats; Cubitt, Andrew B.; Kallio, Karen; Gross, Larry A.; Tsien, Roger Y.; Remington, S. James (1996-09-06). "Crystal Structure of the Aequorea victoria Green Fluorescent Protein". Science. 273 (5280): 1392–1395. Bibcode:1996Sci...273.1392O. doi:10.1126/science.273.5280.1392. ISSN 0036-8075. PMID 8703075. <a href="https://www.science.org/doi/10.1126/science.273.5280.1392" target="_blank">https://www.science.org/doi/10.1126/science.273.5280.1392</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Lambert, Talley. "EYFP at FPbase". FPbase. Retrieved 2021-02-22. <a href="https://www.fpbase.org/protein/eyfp/" target="_blank">https://www.fpbase.org/protein/eyfp/</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Nagai, T; Ibata, K; Park, E. S.; Kubota, M; Mikoshiba, K; Miyawaki, A (2002). "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications". Nature Biotechnology. 20 (1): 87–90. doi:10.1038/nbt0102-87. PMID 11753368. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Ormö, Mats; Cubitt, Andrew B.; Kallio, Karen; Gross, Larry A.; Tsien, Roger Y.; Remington, S. James (1996-09-06). "Crystal Structure of the Aequorea victoria Green Fluorescent Protein". Science. 273 (5280): 1392–1395. Bibcode:1996Sci...273.1392O. doi:10.1126/science.273.5280.1392. ISSN 0036-8075. PMID 8703075. <a href="https://www.science.org/doi/10.1126/science.273.5280.1392" target="_blank">https://www.science.org/doi/10.1126/science.273.5280.1392</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> </ol>