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Granzyme
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<h2 id="history">History</h2> <p>In 1986 <a href="/facts/J%25C3%25BCrg_Tschopp/h680cM9E">Jürg Tschopp</a> and his group published a paper on their discovery of granzymes. In the paper they discussed how they purified, characterized and discovered a variety of granzymes found within cytolytic granules that were carried by cytotoxic T lymphocytes and natural killer cells. Jürg was able to identify 8 different granzymes and discovered partial amino acid sequences for each. The molecules were unofficially named Grs for five years before Jürg and his team came up with the name granzymes which was widely accepted by the scientific community.<a class="footnote-ref" id="fnref:18" href="#fn:18"><sup>18</sup></a> </p><p>Granzyme secretion can be detected and measured using <a href="/facts/Western_Blot/YEr6huPC">Western Blot</a> or <a href="/facts/ELISA/8yvpYDtH">ELISA</a> techniques. Granzyme secreting cells can be identified and quantified by <a href="/facts/Flow_cytometry/M2IvPdL8">flow cytometry</a> or <a href="/facts/ELISPOT/zo2eKIYV">ELISPOT</a>. Alternatively, granzyme activity can be assayed by virtue of their protease activity. </p> <h2 id="other-functions">Other functions</h2> <p>In Cullen's paper “Granzymes in Cancer and Immunity” he discusses how <a href="/facts/GZMA/VjjtNIkB">granzyme A</a> has been known to be found in elevated levels within patients who currently have an infectious disease and/or in a <a href="/facts/Inflammation/Ura86MIy">pro-inflammatory</a> state. Granzymes have also been found to help initiate the inflammatory response. “For example, rheumatoid arthritis patients have increased levels of granzyme A in the <a href="/facts/Synovial_fluid/KbM378Sg">synovial fluid</a> of swollen joints”.<a class="footnote-ref" id="fnref:19" href="#fn:19"><sup>19</sup></a> When granzymes are in an extracellular state they have the ability to activate macrophages and mast cells to initiate the inflammatory response. The interaction between the granzymes and somatic cells are still unexplainable but advances in understanding the process are being made constantly. Other granzymes like <a href="/facts/GZMK/aQ6xlQIE">granzyme K</a> have been found in high levels of patients who have gone septic. <a href="/facts/GZMH/qKnaBKj0">Granzyme H</a> has been found to have a direct correlation with patients who have a viral infection. Scientists are able to conclude that granzyme H specializes in detecting ‘proteolytic degradation’ which is found in viral proteins.<a class="footnote-ref" id="fnref:20" href="#fn:20"><sup>20</sup></a> </p><p>Cullen further states in his paper that granzymes may have a role in <a href="/facts/Homeostasis/rloTGAHa">immunomodulation</a>, or the job of maintaining homeostasis in the immune system during an infection. “In humans, loss of perforin function leads to a syndrome called familial hemophagocytic lymphohistiocytosis […]”.<a class="footnote-ref" id="fnref:21" href="#fn:21"><sup>21</sup></a> This syndrome can lead to death because both T cells and macrophages multiply to fight the pathogen, resulting in harmful levels of proinflammatory cytokines. The overactivation can lead to inflammation of vital organs, anemia via overactivated macrophages phagocytosing blood cells, and can potentially be fatal. </p><p>In <a href="/facts/Joseph_Trapani_(immunologist)/Ncd4cQLX">Trapani</a>'s paper he talks about how granzymes may have other functions, in addition to their ability to fight off infection. Granzyme A contains certain chemicals that allow it to cause proliferation in B cells to reduce the chance of cancer growth and formation. Test on mice have shown that granzyme A and B might not have a direct link to controlling viral infections, but helping accelerate the immune systems response.<a class="footnote-ref" id="fnref:22" href="#fn:22"><sup>22</sup></a> </p> <h2 id="in-cancer-research">In cancer research</h2> <p>In Cullen's paper “Granzymes in Cancer and Immunity” he describes the process of “<a href="/facts/Immune_surveillance/HRCTf1pb">immune surveillance</a> [as] the process whereby precancerous and malignant cells are recognized by the immune system as damaged and are consequently targeted for elimination”.<a class="footnote-ref" id="fnref:23" href="#fn:23"><sup>23</sup></a> For a tumor to progress it requires conditions within the body and surrounding area to be growth-promoting. Almost all people have suitable immune cells to fight off tumors in the body. Studies have shown that the immune system even has the ability to prevent precancerous cells from growing and arbitrate the regression of established tumors. The dangerous thing about cancer cells is they have the ability to inhibit the function of the immune system. Although a tumor may be in its beginning stage and very weak, it may be giving off chemicals that inhibit the function of the immune system allowing it to grow and become harmful. Tests have shown that mice without granzymes and perforins are at high risk to have tumors spread throughout their body.<a class="footnote-ref" id="fnref:24" href="#fn:24"><sup>24</sup></a> </p><p>Tumors have the ability to escape from immune surveillance by secreting immunosuppressive <a href="/facts/TGF-%25CE%25B2/6bD65SWQ">TGF-β</a>. This inhibits proliferation and activation of T cells. TGF-β production is the most potent mechanism of immune avoidance used by tumors. TGF-β inhibits expression of five different cytotoxic genes including perforin, granzyme A, and granzyme B, which then inhibits T cell-mediated tumor clearance. </p><p>Perforin's role in protecting the body against <a href="/facts/Lymphoma/418wcMEl">lymphoma</a> was emphasized when scientists discovered that <a href="/facts/P53/YEGULLgM">p53</a> did not have as big of a role in lymphoma surveillance as its counterpart perforin. Perforin and granzymes have been found to have a directly related ability to protect the body against the formation of different kinds of lymphomas.<a class="footnote-ref" id="fnref:25" href="#fn:25"><sup>25</sup></a> </p> <h2 id="genes">Genes</h2> <ul><li><i><a href="https://www.genenames.org/tools/search/#!/genes?query=GZMA">GZMA</a></i></li> <li><i><a href="https://www.genenames.org/tools/search/#!/genes?query=GZMB">GZMB</a></i></li> <li><i><a href="https://www.genenames.org/tools/search/#!/genes?query=GZMH">GZMH</a></i></li> <li><i><a href="https://www.genenames.org/tools/search/#!/genes?query=GZMK">GZMK</a></i></li> <li><i><a href="https://www.genenames.org/tools/search/#!/genes?query=GZMM">GZMM</a></i></li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Bots M, Medema JP (December 2006). "Granzymes at a glance". Journal of Cell Science. 119 (Pt 24): 5011–4. doi:10.1242/jcs.03239. PMID 17158907. <a href="https://doi.org/10.1242%2Fjcs.03239" target="_blank">https://doi.org/10.1242%2Fjcs.03239</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Walch M, Dotiwala F, Mulik S, et al. (June 2014). "Cytotoxic cells kill intracellular bacteria through granulysin-mediated delivery of granzymes". Cell. 157 (6): 1309–23. doi:10.1016/j.cell.2014.03.062. PMC 4090916. PMID 24906149. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090916" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090916</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Peters PJ, Borst J, Oorschot V, et al. (May 1991). "Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes". The Journal of Experimental Medicine. 173 (5): 1099–109. doi:10.1084/jem.173.5.1099. PMC 2118839. PMID 2022921. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118839" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118839</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Thomas MP, Whangbo J, McCrossan G, et al. (June 2014). "Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins". Journal of Immunology. 192 (11): 5390–7. doi:10.4049/jimmunol.1303296. PMC 4041364. PMID 24771851. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041364" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041364</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Bots M, Medema JP (December 2006). "Granzymes at a glance". Journal of Cell Science. 119 (Pt 24): 5011–4. doi:10.1242/jcs.03239. PMID 17158907. <a href="https://doi.org/10.1242%2Fjcs.03239" target="_blank">https://doi.org/10.1242%2Fjcs.03239</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Walch M, Dotiwala F, Mulik S, et al. (June 2014). "Cytotoxic cells kill intracellular bacteria through granulysin-mediated delivery of granzymes". Cell. 157 (6): 1309–23. doi:10.1016/j.cell.2014.03.062. PMC 4090916. PMID 24906149. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090916" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090916</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Marcet-Palacios M, Duggan BL, Shostak I, et al. (December 2011). "Granzyme B inhibits vaccinia virus production through proteolytic cleavage of eukaryotic initiation factor 4 gamma 3". PLOS Pathogens. 7 (12): e1002447. doi:10.1371/journal.ppat.1002447. PMC 3240606. PMID 22194691. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240606" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240606</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>Thomas MP, Whangbo J, McCrossan G, et al. (June 2014). "Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins". Journal of Immunology. 192 (11): 5390–7. doi:10.4049/jimmunol.1303296. PMC 4041364. PMID 24771851. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041364" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041364</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>Hendel A, Hiebert PR, Boivin WA, Williams SJ, Granville DJ (April 2010). "Granzymes in age-related cardiovascular and pulmonary diseases". Cell Death and Differentiation. 17 (4): 596–606. doi:10.1038/cdd.2010.5. PMID 20139894. <a href="https://doi.org/10.1038%2Fcdd.2010.5" target="_blank">https://doi.org/10.1038%2Fcdd.2010.5</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Hiebert PR, Granville DJ (December 2012). "Granzyme B in injury, inflammation, and repair". Trends in Molecular Medicine. 18 (12): 732–41. doi:10.1016/j.molmed.2012.09.009. PMID 23099058. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> <li id="fn:11"><p>Hiebert PR, Boivin WA, Zhao H, McManus BM, Granville DJ (2013). "Perforin and granzyme B have separate and distinct roles during atherosclerotic plaque development in apolipoprotein E knockout mice". PLOS ONE. 8 (10): e78939. Bibcode:2013PLoSO...878939H. doi:10.1371/journal.pone.0078939. PMC 3811993. PMID 24205352. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811993" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3811993</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></p></li> <li id="fn:12"><p>Chamberlain CM, Ang LS, Boivin WA, et al. (February 2010). "Perforin-independent extracellular granzyme B activity contributes to abdominal aortic aneurysm". The American Journal of Pathology. 176 (2): 1038–49. doi:10.2353/ajpath.2010.090700. PMC 2808106. PMID 20035050. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2808106" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2808106</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></p></li> <li id="fn:13"><p>Hsu I, Parkinson LG, Shen Y, et al. (2014). "Serpina3n accelerates tissue repair in a diabetic mouse model of delayed wound healing". Cell Death & Disease. 5 (10): e1458. doi:10.1038/cddis.2014.423. PMC 4237249. PMID 25299783. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237249" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237249</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></p></li> <li id="fn:14"><p>Hendel A, Hsu I, Granville DJ (July 2014). "Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability". Laboratory Investigation. 94 (7): 716–25. doi:10.1038/labinvest.2014.62. PMC 4074428. PMID 24791744. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074428" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074428</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></p></li> <li id="fn:15"><p>Hsu I, Parkinson LG, Shen Y, et al. (2014). "Serpina3n accelerates tissue repair in a diabetic mouse model of delayed wound healing". Cell Death & Disease. 5 (10): e1458. doi:10.1038/cddis.2014.423. PMC 4237249. PMID 25299783. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237249" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237249</a> <a href="#fnref:15" class="footnote-back-ref">↩</a></p></li> <li id="fn:16"><p>Hiebert PR, Wu D, Granville DJ (October 2013). "Granzyme B degrades extracellular matrix and contributes to delayed wound closure in apolipoprotein E knockout mice". Cell Death and Differentiation. 20 (10): 1404–14. doi:10.1038/cdd.2013.96. PMC 3770318. PMID 23912712. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770318" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770318</a> <a href="#fnref:16" class="footnote-back-ref">↩</a></p></li> <li id="fn:17"><p>Hiebert PR, Boivin WA, Abraham T, Pazooki S, Zhao H, Granville DJ (June 2011). "Granzyme B contributes to extracellular matrix remodeling and skin aging in apolipoprotein E knockout mice". Experimental Gerontology. 46 (6): 489–99. doi:10.1016/j.exger.2011.02.004. PMID 21316440. S2CID 33176028. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:17" class="footnote-back-ref">↩</a></p></li> <li id="fn:18"><p>Ewen CL, Kane KP, Bleackley RC (January 2012). "A quarter century of granzymes". Cell Death and Differentiation. 19 (1): 28–35. doi:10.1038/cdd.2011.153. PMC 3252830. PMID 22052191. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252830" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252830</a> <a href="#fnref:18" class="footnote-back-ref">↩</a></p></li> <li id="fn:19"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:19" class="footnote-back-ref">↩</a></p></li> <li id="fn:20"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:20" class="footnote-back-ref">↩</a></p></li> <li id="fn:21"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:21" class="footnote-back-ref">↩</a></p></li> <li id="fn:22"><p>Trapani JA (2001). "Granzymes: a family of lymphocyte granule serine proteases". Genome Biology. 2 (12): REVIEWS3014. doi:10.1186/gb-2001-2-12-reviews3014. PMC 138995. PMID 11790262. <a href="/wiki/Joseph_Trapani_(immunologist)" target="_blank">/wiki/Joseph_Trapani_(immunologist)</a> <a href="#fnref:22" class="footnote-back-ref">↩</a></p></li> <li id="fn:23"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:23" class="footnote-back-ref">↩</a></p></li> <li id="fn:24"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:24" class="footnote-back-ref">↩</a></p></li> <li id="fn:25"><p>Cullen SP, Brunet M, Martin SJ (April 2010). "Granzymes in cancer and immunity". Cell Death and Differentiation. 17 (4): 616–23. doi:10.1038/cdd.2009.206. PMID 20075940. <a href="https://doi.org/10.1038%2Fcdd.2009.206" target="_blank">https://doi.org/10.1038%2Fcdd.2009.206</a> <a href="#fnref:25" class="footnote-back-ref">↩</a></p></li> </ol>