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Cell engineering
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<h2 id="overview">Overview</h2> <h3>Improving production of natural cellular products</h3> <p>One general form of cell engineering involves altering natural cell production to achieve a more desirable yield or shorter production time.<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> A possible method for changing natural cell production includes boosting or repressing genes that are involved in the <a href="/facts/Metabolism/WxDrm8k5">metabolism</a> of the product. For example, researchers were able to overexpress transporter genes in hamster <a href="/facts/Ovary/z2hG6bNy">ovary</a> cells to increase <a href="/facts/Monoclonal_antibody/4rUBWB87">monoclonal antibody</a> yield.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a> Another approach could involve incorporating biologically foreign genes into an existing cell line. For example, <i><a href="/facts/Escherichia_coli/nR4Gvl56">E.Coli</a></i>, which synthesizes ethanol, can be modified using genes from <i><a href="/facts/Zymomonas_mobilis/2NwrU5et">Zymomonas mobilis</a></i> to make <a href="/facts/Ethanol_fermentation/lpY5qenQ">ethanol fermentation</a> the primary cell fermentation product.<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> </p> <h3>Altering cell requirements</h3> <p>Another beneficial cell modification is the adjustment of <a href="/facts/Substrate_(biology)/EBDd458H">substrate</a> and growth requirements of a cell. By changing cell needs, the raw material cost, equipment expenses, and skill required to grow and maintain cell cultures can be significantly reduced. For example, scientists have used foreign <a href="/facts/Enzyme/DSI1Fh7y">enzymes</a> to engineer a common industrial <a href="/facts/Yeast/eGD24nfI">yeast</a> strain which allows the cells to grow on substrate cheaper than the traditional <a href="/facts/Glucose/oUSkoLCD">glucose</a>.<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a> Because of the biological engineering focus on improving scale-up costs, research in this area is largely focused on the ability of various enzymes to metabolize low-cost substrates.<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a> </p> <h3>Augmenting cells to produce new products</h3> <p>Closely tied with the field of <a href="/facts/Biotechnology/8eFnI6FB">biotechnology</a>, this subject of cell engineering employs <a href="/facts/Recombinant_DNA/dT3PL7M6">recombinant DNA</a> methods to induce cells to construct a desired product such as a <a href="/facts/Protein/Jxmagu3E">protein</a>, <a href="/facts/Antibody/MX8pdTdP">antibody</a>, or <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a>. One of the most notable examples of this subset of cellular engineering is the <a href="/facts/Transformation_(genetics)/n82cCRCu">transformation</a> of <i><a href="/facts/Escherichia_coli/nR4Gvl56">E. Coli</a></i> to <a href="/facts/Transcription_(biology)/bDgp9HDN">transcript</a> and <a href="/facts/Translation_(biology)/JcPC1rth">translate</a> a precursor to <a href="/facts/Insulin/oXG0myt1">insulin</a> which drastically reduced the cost of production.<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a> Similar research was conducted shortly after in 1979 in which <i>E. Coli</i> was transformed to express human growth hormone for use in treatment of pituitary <a href="/facts/Dwarfism/3dLeF5gt">dwarfism</a>.<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a> Finally, much progress has been made in engineering cells to produce <a href="/facts/Antigen/9JlAernG">antigens</a> for the purpose of creating <a href="/facts/Vaccine/QcXzLug2">vaccines</a>.<a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a> </p> <h3>Adjustment of cell properties</h3> <p>Within the focus of bioengineering, various cell modification methods are utilized to alter inherent properties of cells such as growth density, growth rate, growth yield, temperature resistance, freezing tolerance, chemical sensitivity, and vulnerability to pathogens.<a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a> For example, in 1988 one group of researchers from the Illinois Institute of Technology successfully expressed a <i><a href="/facts/Vitreoscilla/5Kp9TDgQ">Vitreoscilla</a></i> hemoglobin gene in <i>E. Coli</i> to create a strain that was more tolerant to low-oxygen conditions such as those found in high density industrial bioreactors.<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a> </p> <h3>Stem cell engineering</h3> <p>One distinct section of cell engineering involves the alteration and tuning of stem cells. Much of the recent research on stem cell therapies and treatments falls under the aforementioned cell engineering methods. Stem cells are unique in that they may differentiate into various other types of cells which may then be altered to produce novel therapeutics or provide a foundation for further cell engineering efforts.<a class="footnote-ref" id="fnref:14" href="#fn:14"><sup>14</sup></a> One example of directed stem cell engineering includes partially differentiating stem cells into <a href="/facts/Muscle_cell/cP7Jea3q">myocytes</a> to enable production of pro-myogenic factors for the treatment of <a href="/facts/Sarcopenia/qFyDWCXc">sarcopenia</a> or muscle disuse atrophy.<a class="footnote-ref" id="fnref:15" href="#fn:15"><sup>15</sup></a> </p> <h2 id="history">History</h2> <p>The phrase "cell engineering" was first used in a published paper in 1968 to describe the process of improving fuel cells.<a class="footnote-ref" id="fnref:16" href="#fn:16"><sup>16</sup></a> The term was then adopted by other papers until the more specific "fuel-cell engineering" was used. </p><p>The first use of the term in a biological context was in 1971 in a paper which describes methods to graft reproductive caps between algae cells.<a class="footnote-ref" id="fnref:17" href="#fn:17"><sup>17</sup></a> Despite the rising popularity of the phrase, there remains unclear boundaries between cell engineering and other forms of biological engineering.<a class="footnote-ref" id="fnref:18" href="#fn:18"><sup>18</sup></a> </p> <h2 id="examples">Examples</h2> <ul><li>Therapeutic <a href="/facts/T_cell/5z6PQ9UN">T cell</a> engineering:<a class="footnote-ref" id="fnref:19" href="#fn:19"><sup>19</sup></a> altering T cells to target cancer-related antigens for treatment</li> <li><a href="/facts/Monoclonal_antibody/4rUBWB87">Monoclonal antibody</a> production:<a class="footnote-ref" id="fnref:20" href="#fn:20"><sup>20</sup></a> improving monoclonal antibody production using engineered cells</li> <li><i>In vivo</i> <a href="/facts/Microbial_cell_factory/EPpEoYbD">cell factories</a>:<a class="footnote-ref" id="fnref:21" href="#fn:21"><sup>21</sup></a> engineering cells to produce therapeutics within the patient's body</li> <li>Directed <a href="/facts/Stem_cell/qgB9dFrI">stem cell</a> differentiation:<a class="footnote-ref" id="fnref:22" href="#fn:22"><sup>22</sup></a> using external factors to direct stem cell differentiation</li> <li><a href="/facts/Antibody%25E2%2580%2593drug_conjugate/STv3FcXw">Antibody Drug Conjugates</a>:<a class="footnote-ref" id="fnref:23" href="#fn:23"><sup>23</sup></a> engineering antibody and cytotoxic drug linkages for disease treatment</li></ul> <h2 id="external-links">External links</h2> <ul><li><a href="https://www.hopkinsmedicine.org/institute_cell_engineering/">Institute for Cell Engineering</a> at Johns Hopkins University School of Medicine</li> <li><a href="https://bioeng.berkeley.edu/research/cell-tissue">Cell & Tissue Engineering</a> at University of California, Berkeley Bioengineering Department</li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Cameron, Douglas C.; Tong, I-Teh (1993-01-01). "Cellular and metabolic engineering". Applied Biochemistry and Biotechnology. 38 (1): 105–140. doi:10.1007/BF02916416. ISSN 1559-0291. PMID 8346901. <a href="https://doi.org/10.1007/BF02916416" target="_blank">https://doi.org/10.1007/BF02916416</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Nerem, Robert M. (1991-09-01). "Cellular engineering". Annals of Biomedical Engineering. 19 (5): 529–545. doi:10.1007/BF02367396. ISSN 1573-9686. PMID 1741530. <a href="https://doi.org/10.1007/BF02367396" target="_blank">https://doi.org/10.1007/BF02367396</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>"cell engineering - Search Results - PubMed". PubMed. Retrieved 2021-11-19. <a href="https://pubmed.ncbi.nlm.nih.gov/?term=cell+engineering" target="_blank">https://pubmed.ncbi.nlm.nih.gov/?term=cell+engineering</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>Cameron, Douglas C.; Tong, I-Teh (1993-01-01). "Cellular and metabolic engineering". Applied Biochemistry and Biotechnology. 38 (1): 105–140. doi:10.1007/BF02916416. ISSN 1559-0291. PMID 8346901. S2CID 28117582. <a href="https://doi.org/10.1007/BF02916416" target="_blank">https://doi.org/10.1007/BF02916416</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> <li id="fn:5"><p>Tabuchi, Hisahiro; Sugiyama, Tomoya; Tanaka, Saeko; Tainaka, Satoshi (2010). "Overexpression of taurine transporter in Chinese hamster ovary cells can enhance cell viability and product yield, while promoting glutamine consumption". Biotechnology and Bioengineering. 107 (6): 998–1003. doi:10.1002/bit.22880. ISSN 1097-0290. PMID 20661907. S2CID 9824980. <a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.22880" target="_blank">https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.22880</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></p></li> <li id="fn:6"><p>Ingram, L O; Conway, T; Clark, D P; Sewell, G W; Preston, J F (1987-10-01). "Genetic engineering of ethanol production in Escherichia coli". Applied and Environmental Microbiology. 53 (10): 2420–2425. Bibcode:1987ApEnM..53.2420I. doi:10.1128/aem.53.10.2420-2425.1987. PMC 204123. PMID 3322191. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204123" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204123</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></p></li> <li id="fn:7"><p>Ledesma-Amaro, Rodrigo; Nicaud, Jean-Marc (October 2016). "Metabolic Engineering for Expanding the Substrate Range of Yarrowia lipolytica". Trends in Biotechnology. 34 (10): 798–809. doi:10.1016/j.tibtech.2016.04.010. ISSN 0167-7799. PMID 27207225. <a href="https://doi.org/10.1016/j.tibtech.2016.04.010" target="_blank">https://doi.org/10.1016/j.tibtech.2016.04.010</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></p></li> <li id="fn:8"><p>Prieto, M A; Perez-Aranda, A; Garcia, J L (1993-04-01). "Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range". Journal of Bacteriology. 175 (7): 2162–2167. doi:10.1128/jb.175.7.2162-2167.1993. PMC 204336. PMID 8458860. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204336" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204336</a> <a href="#fnref:8" class="footnote-back-ref">↩</a></p></li> <li id="fn:9"><p>Goeddel, D. V.; Kleid, D. G.; Bolivar, F.; Heyneker, H. L.; Yansura, D. G.; Crea, R.; Hirose, T.; Kraszewski, A.; Itakura, K.; Riggs, A. D. (1979-01-01). "Expression in Escherichia coli of chemically synthesized genes for human insulin". Proceedings of the National Academy of Sciences. 76 (1): 106–110. Bibcode:1979PNAS...76..106G. doi:10.1073/pnas.76.1.106. ISSN 0027-8424. PMC 382885. PMID 85300. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC382885" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC382885</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></p></li> <li id="fn:10"><p>Goeddel, David V.; Heyneker, Herbert L.; Hozumi, Toyohara; Arentzen, Rene; Itakura, Keiichi; Yansura, Daniel G.; Ross, Michael J.; Miozzari, Giuseppe; Crea, Roberto; Seeburg, Peter H. (October 1979). "Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone". Nature. 281 (5732): 544–548. Bibcode:1979Natur.281..544G. doi:10.1038/281544a0. ISSN 1476-4687. PMID 386136. S2CID 4237998. <a href="https://www.nature.com/articles/281544a0" target="_blank">https://www.nature.com/articles/281544a0</a> <a href="#fnref:10" class="footnote-back-ref">↩</a></p></li> <li id="fn:11"><p>Nascimento, I. P.; Leite, L. C. C. (December 2012). "Recombinant vaccines and the development of new vaccine strategies". Brazilian Journal of Medical and Biological Research. 45 (12): 1102–1111. doi:10.1590/S0100-879X2012007500142. ISSN 0100-879X. PMC 3854212. PMID 22948379. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854212" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854212</a> <a href="#fnref:11" class="footnote-back-ref">↩</a></p></li> <li id="fn:12"><p>Cameron, Douglas C.; Tong, I-Teh (1993-01-01). "Cellular and metabolic engineering". Applied Biochemistry and Biotechnology. 38 (1): 105–140. doi:10.1007/BF02916416. ISSN 1559-0291. PMID 8346901. S2CID 28117582. <a href="https://doi.org/10.1007/BF02916416" target="_blank">https://doi.org/10.1007/BF02916416</a> <a href="#fnref:12" class="footnote-back-ref">↩</a></p></li> <li id="fn:13"><p>Dikshit, Kanak L.; Webster, Dale A. (1988-10-30). "Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli". Gene. 70 (2): 377–386. doi:10.1016/0378-1119(88)90209-0. ISSN 0378-1119. PMID 2850971. <a href="https://dx.doi.org/10.1016/0378-1119%2888%2990209-0" target="_blank">https://dx.doi.org/10.1016/0378-1119%2888%2990209-0</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></p></li> <li id="fn:14"><p>Li, Shengwen Calvin; Wang, Lang; Jiang, Hong; Acevedo, Julyana; Chang, Anthony Christopher; Loudon, William Gunter (2009-03-01). "Stem cell engineering for treatment of heart diseases: Potentials and challenges". Cell Biology International. 33 (3): 255–267. doi:10.1016/j.cellbi.2008.11.009. ISSN 1065-6995. PMID 19084605. S2CID 20486657. <a href="https://doi.org/10.1016%2Fj.cellbi.2008.11.009" target="_blank">https://doi.org/10.1016%2Fj.cellbi.2008.11.009</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></p></li> <li id="fn:15"><p>Fix, Dennis K.; Mahmassani, Ziad S.; Petrocelli, Jonathan J.; de Hart, Naomi M.M.P.; Ferrara, Patrick J.; Painter, Jessie S.; Nistor, Gabriel; Lane, Thomas E.; Keirstead, Hans S.; Drummond, Micah J. (2021-12-01). "Reversal of deficits in aged skeletal muscle during disuse and recovery in response to treatment with a secrotome product derived from partially differentiated human pluripotent stem cells". GeroScience. 43 (6): 2635–2652. doi:10.1007/s11357-021-00423-0. ISSN 2509-2723. PMC 8602548. PMID 34427856. <a href="https://doi.org/10.1007/s11357-021-00423-0" target="_blank">https://doi.org/10.1007/s11357-021-00423-0</a> <a href="#fnref:15" class="footnote-back-ref">↩</a></p></li> <li id="fn:16"><p>Thurber, W. C. (1968-05-01). "Closure to "Discussions of 'A Fuel Cell Power Plant for a Deep Diving Submarine'" (1968, ASME J. Eng. Ind., 90, pp. 266–267)". Journal of Engineering for Industry. 90 (2): 267. doi:10.1115/1.3604626. ISSN 0022-0817. <a href="https://doi.org/10.1115%2F1.3604626" target="_blank">https://doi.org/10.1115%2F1.3604626</a> <a href="#fnref:16" class="footnote-back-ref">↩</a></p></li> <li id="fn:17"><p>Bonotto, S.; Kirchmann, R.; Manil, P. (1971-01-01). "Cell Engineering in Acetabularia: A Graft Method for Obtaining Large Cells with Two or More Reproductive Caps". Giornale Botanico Italiano. 105 (1): 1–9. doi:10.1080/11263507109431460. ISSN 0017-0070. <a href="https://doi.org/10.1080/11263507109431460" target="_blank">https://doi.org/10.1080/11263507109431460</a> <a href="#fnref:17" class="footnote-back-ref">↩</a></p></li> <li id="fn:18"><p>Cameron, Douglas C.; Tong, I-Teh (1993-01-01). "Cellular and metabolic engineering". Applied Biochemistry and Biotechnology. 38 (1): 105–140. doi:10.1007/BF02916416. ISSN 1559-0291. PMID 8346901. S2CID 28117582. <a href="https://doi.org/10.1007/BF02916416" target="_blank">https://doi.org/10.1007/BF02916416</a> <a href="#fnref:18" class="footnote-back-ref">↩</a></p></li> <li id="fn:19"><p>Sadelain, Michel; Rivière, Isabelle; Riddell, Stanley (May 2017). "Therapeutic T cell engineering". Nature. 545 (7655): 423–431. Bibcode:2017Natur.545..423S. doi:10.1038/nature22395. ISSN 1476-4687. PMC 5632949. PMID 28541315. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632949" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632949</a> <a href="#fnref:19" class="footnote-back-ref">↩</a></p></li> <li id="fn:20"><p>Rita Costa, A.; Elisa Rodrigues, M.; Henriques, Mariana; Azeredo, Joana; Oliveira, Rosário (2010-02-01). "Guidelines to cell engineering for monoclonal antibody production". European Journal of Pharmaceutics and Biopharmaceutics. 74 (2): 127–138. doi:10.1016/j.ejpb.2009.10.002. hdl:1822/11473. ISSN 0939-6411. PMID 19853660. <a href="https://www.sciencedirect.com/science/article/pii/S0939641109003166" target="_blank">https://www.sciencedirect.com/science/article/pii/S0939641109003166</a> <a href="#fnref:20" class="footnote-back-ref">↩</a></p></li> <li id="fn:21"><p>Mansouri, Maysam; Fussenegger, Martin (2021-09-29). "Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells". Protein & Cell. 13 (7): 476–489. doi:10.1007/s13238-021-00876-1. ISSN 1674-8018. PMC 9226217. PMID 34586617. S2CID 238217661. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226217" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226217</a> <a href="#fnref:21" class="footnote-back-ref">↩</a></p></li> <li id="fn:22"><p>Clause, Kelly C.; Liu, Li J.; Tobita, Kimimasa (April 2010). "Directed Stem Cell Differentiation: The Role of Physical Forces". Cell Communication & Adhesion. 17 (2): 48–54. doi:10.3109/15419061.2010.492535. ISSN 1541-9061. PMC 3285265. PMID 20560867. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285265" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285265</a> <a href="#fnref:22" class="footnote-back-ref">↩</a></p></li> <li id="fn:23"><p>Pettinato, Mark C. (2021-10-27). "Introduction to Antibody-Drug Conjugates". Antibodies. 10 (4): 42. doi:10.3390/antib10040042. ISSN 2073-4468. PMC 8628511. PMID 34842621. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628511" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628511</a> <a href="#fnref:23" class="footnote-back-ref">↩</a></p></li> </ol>