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TBE buffer
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<h2 id="recipe-1-liter-of-5x-stock-solution">Recipe (1 liter of 5X stock solution)</h2> <ul><li>54 g of <a href="/facts/Tris/1nuYMVDA">Tris base</a> (CAS# 77-86-1, free base)</li> <li>27.5 g of <a href="/facts/Boric_acid/0so68XIK">boric acid</a> (CAS# 10043-35-3)</li> <li>20 ml of 0.5 M <a href="/facts/EDTA/XvHgKQyD">EDTA</a> (CAS# 60-00-4) (<a href="/facts/PH/RnSv8D7y">pH</a> 8.0)</li></ul> <p>Adjust pH to 8.3 by HCl.<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> </p><p>TBE can be diluted to 1X prior to use in electrophoresis, 0.5x is acceptable as well. Higher concentrations will result in poor results due to excessive heat generation. </p> <h2 id="see-also">See also</h2> <ul><li><a href="/facts/LB_buffer/cJK9T6TW">LB buffer</a>, lithium borate buffer, a similar buffer containing lithium ions in place of Tris</li> <li><a href="/facts/TAE_buffer/Embv1Xpu">TAE buffer</a>, a similar buffer containing acetic acid in place of boric acid</li></ul> <h2 id="notes">Notes</h2> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>5 mM disodium borate decahydrate or 10 mM sodium hydroxide, pH adjusted to 8.5 with boric acid. <a href="/wiki/Borax" target="_blank">/wiki/Borax</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li> <li id="fn:2"><p>Brody, J.R.; Kern, S.E. (2004). "History and principles of conductive media for standard DNA electrophoresis" (PDF). Anal Biochem. 333 (1): 1–13. doi:10.1016/j.ab.2004.05.054. PMID 15351274. <a href="http://www.cc.ahs.chula.ac.th/Molmed/DNA%20electro%27s%20conductive%20media.pdf" target="_blank">http://www.cc.ahs.chula.ac.th/Molmed/DNA%20electro%27s%20conductive%20media.pdf</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li> <li id="fn:3"><p>Brody, Jonathan R.; Kern, Scott E. (February 2004). "Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis". BioTechniques. 36 (2): 214–216. doi:10.2144/04362BM02. <a href="/wiki/Doi_(identifier)" target="_blank">/wiki/Doi_(identifier)</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li> <li id="fn:4"><p>"5X TBE(1.1M Tris; 900mM Borate; 25mM EDTA; pH 8.3)" (PDF). Archived from the original (PDF) on 2017-11-07. <a href="https://web.archive.org/web/20171107223153/https://www.eeb.ucla.edu/Faculty/Barber/Web%20Protocols/Protocol7.pdf" target="_blank">https://web.archive.org/web/20171107223153/https://www.eeb.ucla.edu/Faculty/Barber/Web%20Protocols/Protocol7.pdf</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></p></li> </ol>