Absorbance

<h2 id="history-and-uses-of-the-term-absorbance">History and uses of the term absorbance</h2>
<h3>Beer-Lambert law</h3>
The roots of the term absorbance are in the <a href="/facts/Beer-Lambert_law/nl64rI1C">Beer–Lambert law</a>. As light moves through a medium, it will become dimmer as it is being "extinguished". Bouguer recognized that this extinction (now often called attenuation) was not linear with distance traveled through the medium, but related by what we now refer to as an exponential function.
If 
 
 
 
 
 I
 
 0
 
 
 
 
 {\displaystyle I_{0}}
 
 is the intensity of the light at the beginning of the travel and 
 
 
 
 
 I
 
 d
 
 
 
 
 {\displaystyle I_{d}}
 
 is the intensity of the light detected after travel of a distance 
 
 
 
 d
 
 
 {\displaystyle d}
 
, the fraction transmitted, 
 
 
 
 T
 
 
 {\displaystyle T}
 
, is given by

 
 
 
 T
 =
 
 
 
 I
 
 d
 
 
 
 I
 
 0
 
 
 
 
 =
 exp
 ⁡
 (
 −
 μ
 d
 )
 
 ,
 
 
 {\displaystyle T={\frac {I_{d}}{I_{0}}}=\exp(-\mu d)\,,}

where 
 
 
 
 μ
 
 
 {\displaystyle \mu }
 
 is called an <a href="/facts/Propagation_constant/3XpFb0q1">attenuation constant</a> (a term used in various fields where a signal is transmitted though a medium) or coefficient. The amount of light transmitted is falling off exponentially with distance. Taking the natural logarithm in the above equation, we get

 
 
 
 −
 ln
 ⁡
 (
 T
 )
 =
 ln
 ⁡
 
 
 
 I
 
 0
 
 
 
 I
 
 d
 
 
 
 
 =
 μ
 d
 
 .
 
 
 {\displaystyle -\ln(T)=\ln {\frac {I_{0}}{I_{d}}}=\mu d\,.}

For scattering media, the constant is often divided into two parts,<a class="footnote-ref" id="fnref:4" href="#fn:4">4</a> 
 
 
 
 μ
 =
 
 μ
 
 s
 
 
 +
 
 μ
 
 a
 
 
 
 
 {\displaystyle \mu =\mu _{s}+\mu _{a}}
 
, separating it into a scattering coefficient 
 
 
 
 
 μ
 
 s
 
 
 
 
 {\displaystyle \mu _{s}}
 
 and an absorption coefficient 
 
 
 
 
 μ
 
 a
 
 
 
 
 {\displaystyle \mu _{a}}
 
, obtaining

 
 
 
 −
 ln
 ⁡
 (
 T
 )
 =
 ln
 ⁡
 
 
 
 I
 
 0
 
 
 
 I
 
 s
 
 
 
 
 =
 (
 
 μ
 
 s
 
 
 +
 
 μ
 
 a
 
 
 )
 d
 
 .
 
 
 {\displaystyle -\ln(T)=\ln {\frac {I_{0}}{I_{s}}}=(\mu _{s}+\mu _{a})d\,.}

If a size of a detector is very small compared to the distance traveled by the light, any light that is scattered by a particle, either in the forward or backward direction, will not strike the detector. (Bouguer was studying astronomical phenomena, so this condition was met.) In such case, a plot of 
 
 
 
 −
 ln
 ⁡
 (
 T
 )
 
 
 {\displaystyle -\ln(T)}
 
 as a function of wavelength will yield a superposition of the effects of absorption and scatter. Because the absorption portion is more distinct and tends to ride on a background of the scatter portion, it is often used to identify and quantify the absorbing species. Consequently, this is often referred to as <a href="/facts/Absorption_spectroscopy/GH7RC14D">absorption spectroscopy</a>, and the plotted quantity is called "absorbance", symbolized as 
 
 
 
 
 A
 
 
 
 {\displaystyle \mathrm {A} }
 
. Some disciplines by convention use decadic (base 10) absorbance rather than Napierian (natural) absorbance, resulting in: 
 
 
 
 
 
 A
 
 
 10
 
 
 =
 
 μ
 
 10
 
 
 d
 
 
 {\displaystyle \mathrm {A} _{10}=\mu _{10}d}
 
 (with the subscript 10 usually not shown).

<h3>Absorbance for non-scattering samples</h3>
Within a homogeneous medium such as a solution, there is no scattering. For this case, researched extensively by <a href="/facts/August_Beer/rFpXl1Mk">August Beer</a>, the concentration of the absorbing species follows the same linear contribution to absorbance as the path-length. Additionally, the contributions of individual absorbing species are additive. This is a very favorable situation, and made absorbance an absorption metric far preferable to absorption fraction (absorptance). This is the case for which the term "absorbance" was first used.
A common expression of the <a href="/facts/Beer%2527s_law/nl64rI1C">Beer's law</a> relates the attenuation of light in a material as: 
 
 
 
 
 A
 
 =
 ε
 ℓ
 c
 
 
 {\displaystyle \mathrm {A} =\varepsilon \ell c}
 
, where 
 
 
 
 
 A
 
 
 
 {\displaystyle \mathrm {A} }
 
 is the absorbance; 
 
 
 
 ε
 
 
 {\displaystyle \varepsilon }
 
 is the <a href="/facts/Molar_attenuation_coefficient/TwglQEAW">molar attenuation coefficient</a> or <a href="/facts/Molar_absorptivity/TwglQEAW">absorptivity</a> of the attenuating species; 
 
 
 
 ℓ
 
 
 {\displaystyle \ell }
 
 is the optical path length; and 
 
 
 
 c
 
 
 {\displaystyle c}
 
 is the concentration of the attenuating species.

<h3>Absorbance for scattering samples</h3>
For samples which scatter light, absorbance is defined as "the negative logarithm of one minus absorptance (absorption fraction: 
 
 
 
 α
 
 
 {\displaystyle \alpha }
 
) as measured on a uniform sample".<a class="footnote-ref" id="fnref:5" href="#fn:5">5</a> For decadic absorbance,<a class="footnote-ref" id="fnref:6" href="#fn:6">6</a> this may be symbolized as 
 
 
 
 
 
 A
 
 
 10
 
 
 =
 −
 
 log
 
 10
 
 
 ⁡
 (
 1
 −
 α
 )
 
 
 {\displaystyle \mathrm {A} _{10}=-\log _{10}(1-\alpha )}
 
. If a sample both transmits and <a href="/facts/Diffuse_reflectance_spectroscopy/IGfRaCua">remits light</a>, and is not luminescent, the fraction of light absorbed (
 
 
 
 α
 
 
 {\displaystyle \alpha }
 
), remitted (
 
 
 
 R
 
 
 {\displaystyle R}
 
), and transmitted (
 
 
 
 T
 
 
 {\displaystyle T}
 
) add to 1: 
 
 
 
 α
 +
 R
 +
 T
 =
 1
 
 
 {\displaystyle \alpha +R+T=1}
 
. Note that 
 
 
 
 1
 −
 α
 =
 R
 +
 T
 
 
 {\displaystyle 1-\alpha =R+T}
 
, and the formula may be written as 
 
 
 
 
 
 A
 
 
 10
 
 
 =
 −
 
 log
 
 10
 
 
 ⁡
 (
 R
 +
 T
 )
 
 
 {\displaystyle \mathrm {A} _{10}=-\log _{10}(R+T)}
 
. For a sample which does not scatter, 
 
 
 
 R
 =
 0
 
 
 {\displaystyle R=0}
 
, and 
 
 
 
 1
 −
 α
 =
 T
 
 
 {\displaystyle 1-\alpha =T}
 
, yielding the formula for absorbance of a material discussed below.
Even though this absorbance function is very useful with scattering samples, the function does not have the same desirable characteristics as it does for non-scattering samples. There is, however, a property called <a href="/facts/Representative_layer_theory/bE715GUu">absorbing power</a> which may be estimated for these samples. The <a href="/facts/Representative_layer_theory/bE715GUu">absorbing power</a> of a single unit thickness of material making up a scattering sample is the same as the absorbance of the same thickness of the material in the absence of scatter.<a class="footnote-ref" id="fnref:7" href="#fn:7">7</a>

<h3>Optics</h3>
In <a href="/facts/Optics/p0vq4S1T">optics</a>, absorbance or decadic absorbance is the <a href="/facts/Common_logarithm/q6EhDnWk">common logarithm</a> of the ratio of incident to transmitted <a href="/facts/Radiant_power/ak2d0btz">radiant power</a> through a material, and spectral absorbance or spectral decadic absorbance is the common logarithm of the ratio of incident to transmitted <a href="/facts/Radiant_power/ak2d0btz">spectral radiant power</a> through a material. Absorbance is <a href="/facts/Dimensionless_quantity/TINrpQIq">dimensionless</a>, and in particular is not a length, though it is a monotonically increasing function of path length, and approaches zero as the path length approaches zero.

<h2 id="mathematical-definitions">Mathematical definitions</h2>
<h3>Absorbance of a material</h3>
The absorbance of a material, denoted A, is given by<a class="footnote-ref" id="fnref:8" href="#fn:8">8</a>

 
 
 
 A
 =
 
 log
 
 10
 
 
 ⁡
 
 
 
 Φ
 
 e
 
 
 i
 
 
 
 Φ
 
 e
 
 
 t
 
 
 
 
 =
 −
 
 log
 
 10
 
 
 ⁡
 T
 ,
 
 
 {\displaystyle A=\log _{10}{\frac {\Phi _{\text{e}}^{\text{i}}}{\Phi _{\text{e}}^{\text{t}}}}=-\log _{10}T,}

where

<ul><li>
 
 
 
 
 Φ
 
 e
 
 
 t
 
 
 
 
 {\textstyle \Phi _{\text{e}}^{\text{t}}}
 
 is the <a href="/facts/Radiant_flux/ak2d0btz">radiant flux</a> transmitted by that material,</li>
<li>
 
 
 
 
 Φ
 
 e
 
 
 i
 
 
 
 
 {\textstyle \Phi _{\text{e}}^{\text{i}}}
 
 is the <a href="/facts/Radiant_flux/ak2d0btz">radiant flux</a> received by that material, and</li>
<li>
 
 
 
 T
 =
 
 Φ
 
 e
 
 
 t
 
 
 
 /
 
 
 Φ
 
 e
 
 
 i
 
 
 
 
 {\textstyle T=\Phi _{\text{e}}^{\text{t}}/\Phi _{\text{e}}^{\text{i}}}
 
 is the <a href="/facts/Transmittance/xlyCG7ks">transmittance</a> of that material.</li></ul>
Absorbance is a <a href="/facts/Dimensionless/TINrpQIq">dimensionless</a> quantity. Nevertheless, the absorbance unit or AU is commonly used in <a href="/facts/Ultraviolet%25E2%2580%2593visible_spectroscopy/MUPLLczo">ultraviolet–visible spectroscopy</a> and its <a href="/facts/High-performance_liquid_chromatography/UNT9jPp0">high-performance liquid chromatography</a> applications, often in derived units such as the milli-absorbance unit (mAU) or milli-absorbance unit-minutes (mAU×min), a unit of absorbance integrated over time.<a class="footnote-ref" id="fnref:9" href="#fn:9">9</a>
Absorbance is related to <a href="/facts/Optical_depth/XQwtIdFH">optical depth</a> by

 
 
 
 A
 =
 
 
 τ
 
 ln
 ⁡
 10
 
 
 
 =
 τ
 
 log
 
 10
 
 
 ⁡
 e
 
 ,
 
 
 {\displaystyle A={\frac {\tau }{\ln 10}}=\tau \log _{10}e\,,}

where τ is the optical depth.

<h3>Spectral absorbance</h3>
Spectral absorbance in frequency and spectral absorbance in wavelength of a material, denoted Aν and Aλ respectively, are given by<a class="footnote-ref" id="fnref:10" href="#fn:10">10</a>

 
 
 
 
 
 
 
 
 A
 
 ν
 
 
 
 
 
 =
 
 log
 
 10
 
 
 ⁡
 
 
 
 Φ
 
 
 e
 
 ,
 ν
 
 
 i
 
 
 
 Φ
 
 
 e
 
 ,
 ν
 
 
 t
 
 
 
 
 =
 −
 
 log
 
 10
 
 
 ⁡
 
 T
 
 ν
 
 
 
 ,
 
 
 
 
 
 A
 
 λ
 
 
 
 
 
 =
 
 log
 
 10
 
 
 ⁡
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 i
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 t
 
 
 
 
 =
 −
 
 log
 
 10
 
 
 ⁡
 
 T
 
 λ
 
 
 
 ,
 
 
 
 
 
 
 {\displaystyle {\begin{aligned}A_{\nu }&=\log _{10}{\frac {\Phi _{{\text{e}},\nu }^{\text{i}}}{\Phi _{{\text{e}},\nu }^{\text{t}}}}=-\log _{10}T_{\nu }\,,\\A_{\lambda }&=\log _{10}{\frac {\Phi _{{\text{e}},\lambda }^{\text{i}}}{\Phi _{{\text{e}},\lambda }^{\text{t}}}}=-\log _{10}T_{\lambda }\,,\end{aligned}}}

where

<ul><li>
 
 
 
 
 Φ
 
 
 e
 
 ,
 ν
 
 
 t
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} ,\nu }^{t}}
 
 is the <a href="/facts/Radiant_flux/ak2d0btz">spectral radiant flux in frequency</a> transmitted by that material;</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 ,
 ν
 
 
 i
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} ,\nu }^{i}}
 
 is the spectral radiant flux in frequency received by that material;</li>
<li>
 
 
 
 
 T
 
 ν
 
 
 
 
 {\textstyle T_{\nu }}
 
 is the <a href="/facts/Transmittance/xlyCG7ks">spectral transmittance in frequency</a> of that material;</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 t
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} ,\lambda }^{t}}
 
 is the <a href="/facts/Radiant_flux/ak2d0btz">spectral radiant flux in wavelength</a> transmitted by that material;</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 i
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} ,\lambda }^{i}}
 
 is the spectral radiant flux in wavelength received by that material; and</li>
<li>
 
 
 
 
 T
 
 λ
 
 
 
 
 {\textstyle T_{\lambda }}
 
 is the <a href="/facts/Transmittance/xlyCG7ks">spectral transmittance in wavelength</a> of that material.</li></ul>
Spectral absorbance is related to spectral optical depth by

 
 
 
 
 
 
 
 
 A
 
 ν
 
 
 
 
 
 =
 
 
 
 τ
 
 ν
 
 
 
 ln
 ⁡
 10
 
 
 
 =
 
 τ
 
 ν
 
 
 
 log
 
 10
 
 
 ⁡
 e
 
 ,
 
 
 
 
 
 A
 
 λ
 
 
 
 
 
 =
 
 
 
 τ
 
 λ
 
 
 
 ln
 ⁡
 10
 
 
 
 =
 
 τ
 
 λ
 
 
 
 log
 
 10
 
 
 ⁡
 e
 
 ,
 
 
 
 
 
 
 {\displaystyle {\begin{aligned}A_{\nu }&={\frac {\tau _{\nu }}{\ln 10}}=\tau _{\nu }\log _{10}e\,,\\A_{\lambda }&={\frac {\tau _{\lambda }}{\ln 10}}=\tau _{\lambda }\log _{10}e\,,\end{aligned}}}

where

<ul><li>τν is the spectral optical depth in frequency, and</li>
<li>τλ is the spectral optical depth in wavelength.</li></ul>
Although absorbance is properly unitless, it is sometimes reported in "absorbance units", or AU. Many people, including scientific researchers, wrongly state the results from absorbance measurement experiments in terms of these made-up units.<a class="footnote-ref" id="fnref:11" href="#fn:11">11</a>

<h2 id="relationship-with-attenuation">Relationship with attenuation</h2>
<h3>Attenuance</h3>
Absorbance is a number that measures the attenuation of the transmitted radiant power in a material. Attenuation can be caused by the physical process of "absorption", but also reflection, scattering, and other physical processes. Absorbance of a material is approximately equal to its attenuance when both the absorbance is much less than 1 and the emittance of that material (not to be confused with <a href="/facts/Radiant_exitance/US1T54p8">radiant exitance</a> or <a href="/facts/Emissivity/LUxfMRRI">emissivity</a>) is much less than the absorbance. Indeed,

 
 
 
 
 Φ
 
 
 e
 
 
 
 
 t
 
 
 
 +
 
 Φ
 
 
 e
 
 
 
 
 a
 t
 t
 
 
 
 =
 
 Φ
 
 
 e
 
 
 
 
 i
 
 
 
 +
 
 Φ
 
 
 e
 
 
 
 
 e
 
 
 
 
 ,
 
 
 {\displaystyle \Phi _{\mathrm {e} }^{\mathrm {t} }+\Phi _{\mathrm {e} }^{\mathrm {att} }=\Phi _{\mathrm {e} }^{\mathrm {i} }+\Phi _{\mathrm {e} }^{\mathrm {e} }\,,}

where

<ul><li>
 
 
 
 
 Φ
 
 
 e
 
 
 
 
 t
 
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} }^{\mathrm {t} }}
 
 is the radiant power transmitted by that material,</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 
 
 
 a
 t
 t
 
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} }^{\mathrm {att} }}
 
 is the radiant power attenuated by that material,</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 
 
 
 i
 
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} }^{\mathrm {i} }}
 
 is the radiant power received by that material, and</li>
<li>
 
 
 
 
 Φ
 
 
 e
 
 
 
 
 e
 
 
 
 
 
 {\textstyle \Phi _{\mathrm {e} }^{\mathrm {e} }}
 
 is the radiant power emitted by that material.</li></ul>
This is equivalent to

 
 
 
 T
 +
 
 A
 T
 T
 
 =
 1
 +
 E
 
 ,
 
 
 {\displaystyle T+\mathrm {ATT} =1+E\,,}

where

<ul><li>
 
 
 
 T
 =
 
 Φ
 
 
 e
 
 
 
 
 t
 
 
 
 
 /
 
 
 Φ
 
 
 e
 
 
 
 
 i
 
 
 
 
 
 {\textstyle T=\Phi _{\mathrm {e} }^{\mathrm {t} }/\Phi _{\mathrm {e} }^{\mathrm {i} }}
 
 is the transmittance of that material,</li>
<li>
 
 
 
 
 A
 T
 T
 
 =
 
 Φ
 
 
 e
 
 
 
 
 a
 t
 t
 
 
 
 
 /
 
 
 Φ
 
 
 e
 
 
 
 
 i
 
 
 
 
 
 {\textstyle \mathrm {ATT} =\Phi _{\mathrm {e} }^{\mathrm {att} }/\Phi _{\mathrm {e} }^{\mathrm {i} }}
 
 is the attenuance of that material,</li>
<li>
 
 
 
 E
 =
 
 Φ
 
 
 e
 
 
 
 
 e
 
 
 
 
 /
 
 
 Φ
 
 
 e
 
 
 
 
 i
 
 
 
 
 
 {\textstyle E=\Phi _{\mathrm {e} }^{\mathrm {e} }/\Phi _{\mathrm {e} }^{\mathrm {i} }}
 
 is the emittance of that material.</li></ul>
According to the <a href="/facts/Beer%25E2%2580%2593Lambert_law/nl64rI1C">Beer–Lambert law</a>, T = 10−A, so

<ul><li>
 
 
 
 
 A
 T
 T
 
 =
 1
 −
 
 10
 
 −
 A
 
 
 +
 E
 ≈
 A
 ln
 ⁡
 10
 +
 E
 ,
 
 
 if
 
  
 A
 ≪
 1
 ,
 
 
 {\displaystyle \mathrm {ATT} =1-10^{-A}+E\approx A\ln 10+E,\quad {\text{if}}\ A\ll 1,}
 
</li></ul>
and finally

<ul><li>
 
 
 
 
 A
 T
 T
 
 ≈
 A
 ln
 ⁡
 10
 ,
 
 
 if
 
  
 E
 ≪
 A
 .
 
 
 {\displaystyle \mathrm {ATT} \approx A\ln 10,\quad {\text{if}}\ E\ll A.}
 
</li></ul>
<h3>Attenuation coefficient</h3>
Absorbance of a material is also related to its <a href="/facts/Attenuation_coefficient/ppCIsSGw">decadic attenuation coefficient</a> by

 
 
 
 A
 =
 
 ∫
 
 0
 
 
 l
 
 
 a
 (
 z
 )
 
 
 d
 
 z
 
 ,
 
 
 {\displaystyle A=\int _{0}^{l}a(z)\,\mathrm {d} z\,,}

where

<ul><li>l is the thickness of that material through which the light travels, and</li>
<li>a(z) is the decadic attenuation coefficient of that material at z.</li></ul>
If a(z) is uniform along the path, the attenuation is said to be a linear attenuation, and the relation becomes

A
        =
        a
        l
        .
      
    
    {\displaystyle A=al.}

Sometimes the relation is given using the <a href="/facts/Molar_attenuation_coefficient/TwglQEAW">molar attenuation coefficient</a> of the material, that is its attenuation coefficient divided by its <a href="/facts/Molar_concentration/P2WL5b9q">molar concentration</a>:

 
 
 
 A
 =
 
 ∫
 
 0
 
 
 l
 
 
 ε
 c
 (
 z
 )
 
 
 d
 
 z
 
 ,
 
 
 {\displaystyle A=\int _{0}^{l}\varepsilon c(z)\,\mathrm {d} z\,,}

where

<ul><li>ε is the molar attenuation coefficient of that material, and</li>
<li>c(z) is the molar concentration of that material at z.</li></ul>
If c(z) is uniform along the path, the relation becomes

 
 
 
 A
 =
 ε
 c
 l
 
 .
 
 
 {\displaystyle A=\varepsilon cl\,.}

The use of the term "molar absorptivity" for molar attenuation coefficient is discouraged.<a class="footnote-ref" id="fnref:12" href="#fn:12">12</a>

<h2 id="measurements">Measurements</h2>
<h3>Logarithmic vs. directly proportional measurements</h3>
The amount of light transmitted through a material diminishes <a href="/facts/Exponential_function/ewlYxvR2">exponentially</a> as it travels through the material, according to the Beer–Lambert law (A = (ε)(l)). Since the absorbance of a sample is measured as a logarithm, it is directly proportional to the thickness of the sample and to the concentration of the absorbing material in the sample. Some other measures related to absorption, such as transmittance, are measured as a simple ratio so they vary exponentially with the thickness and concentration of the material.

Absorbances and equivalent transmittances<table><tbody><tr><th scope="col">Absorbance: − log 10 ⁡ ( Φ e t / Φ e i ) {\textstyle -\log _{10}\left(\Phi _{\mathrm {e} }^{\mathrm {t} }/\Phi _{\mathrm {e} }^{\mathrm {i} }\right)} </th><th scope="col">Transmittance: Φ e t / Φ e i {\textstyle \Phi _{\mathrm {e} }^{\mathrm {t} }/\Phi _{\mathrm {e} }^{\mathrm {i} }} </th></tr><tr><td>0</td><td>1</td></tr><tr><td>0.1</td><td>0.79</td></tr><tr><td>0.25</td><td>0.56</td></tr><tr><td>0.5</td><td>0.32</td></tr><tr><td>0.75</td><td>0.18</td></tr><tr><td>0.9</td><td>0.13</td></tr><tr><td>1</td><td>0.1</td></tr><tr><td>2</td><td>0.01</td></tr><tr><td>3</td><td>0.001</td></tr></tbody></table>
<h3>Instrument measurement range</h3>
Any real measuring instrument has a limited range over which it can accurately measure absorbance. An instrument must be calibrated and checked against known standards if the readings are to be trusted. Many instruments will become non-linear (fail to follow the Beer–Lambert law) starting at approximately 2 AU (~1% transmission). It is also difficult to accurately measure very small absorbance values (below 10−4) with commercially available instruments for chemical analysis. In such cases, <a href="/facts/Laser_absorption_spectrometry/Mlkdxzu8">laser-based absorption techniques</a> can be used, since they have demonstrated detection limits that supersede those obtained by conventional non-laser-based instruments by many orders of magnitude (detection has been demonstrated all the way down to 5×10−13). The theoretical best accuracy for most commercially available non-laser-based instruments is attained in the range near 1 AU. The path length or concentration should then, when possible, be adjusted to achieve readings near this range.

<h3>Method of measurement</h3>
Typically, absorbance of a dissolved substance is measured using <a href="/facts/Absorption_spectroscopy/GH7RC14D">absorption spectroscopy</a>. This involves shining a light through a solution and recording how much light and what wavelengths were transmitted onto a detector. Using this information, the wavelengths that were absorbed can be determined.<a class="footnote-ref" id="fnref:13" href="#fn:13">13</a> First, measurements on a "blank" are taken using just the solvent for reference purposes. This is so that the absorbance of the solvent is known, and then any change in absorbance when measuring the whole solution is made by just the solute of interest. Then measurements of the solution are taken. The transmitted spectral radiant flux that makes it through the solution sample is measured and compared to the incident spectral radiant flux. As stated above, the spectral absorbance at a given wavelength is

 
 
 
 
 A
 
 λ
 
 
 =
 
 log
 
 10
 
 
 
 
 (
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 
 i
 
 
 
 
 Φ
 
 
 e
 
 ,
 λ
 
 
 
 t
 
 
 
 
 
 )
 
 
 .
 
 
 {\displaystyle A_{\lambda }=\log _{10}\!\left({\frac {\Phi _{\mathrm {e} ,\lambda }^{\mathrm {i} }}{\Phi _{\mathrm {e} ,\lambda }^{\mathrm {t} }}}\right)\!.}

The absorbance spectrum is plotted on a graph of absorbance vs. wavelength.<a class="footnote-ref" id="fnref:14" href="#fn:14">14</a>
An <a href="/facts/Ultraviolet-visible_spectroscopy/MUPLLczo">Ultraviolet-visible spectroscopy#Ultraviolet–visible spectrophotometer</a> will do all this automatically. To use this machine, solutions are placed in a small <a href="/facts/Cuvette/SpAJp2Nx">cuvette</a> and inserted into the holder. The machine is controlled through a computer and, once it has been "blanked", automatically displays the absorbance plotted against wavelength. Getting the absorbance spectrum of a solution is useful for determining the concentration of that solution using the Beer–Lambert law and is used in <a href="/facts/HPLC/UNT9jPp0">HPLC</a>.

<h2 id="shade-number">Shade number</h2>
Some filters, notably <a href="/facts/Welding/6EJ62mYV">welding</a> glass, are rated by shade number (SN), which is 7/3 times the absorbance plus one:<a class="footnote-ref" id="fnref:15" href="#fn:15">15</a>

 
 
 
 
 
 
 
 
 S
 N
 
 
 
 
 =
 
 
 7
 3
 
 
 A
 +
 1
 
 
 
 
 
 
 =
 
 
 7
 3
 
 
 (
 −
 
 log
 
 10
 
 
 ⁡
 T
 )
 +
 1
 
 .
 
 
 
 
 
 
 {\displaystyle {\begin{aligned}\mathrm {SN} &={\frac {7}{3}}A+1\\&={\frac {7}{3}}(-\log _{10}T)+1\,.\end{aligned}}}

For example, if the filter has 0.1% transmittance (0.001 transmittance, which is 3 absorbance units), its shade number would be 8.

<h2 id="see-also">See also</h2>
<ul><li><a href="/facts/Absorptance/n6C9pnDR">Absorptance</a></li>
<li><a href="/facts/Tunable_Diode_Laser_Absorption_Spectroscopy/hHa6YSq6">Tunable Diode Laser Absorption Spectroscopy</a> (TDLAS)</li>
<li><a href="/facts/Densitometry/eSkoaTNz">Densitometry</a></li>
<li><a href="/facts/Neutral_density_filter/3BaETEVK">Neutral density filter</a></li>
<li><a href="/facts/Mathematical_descriptions_of_opacity/vv0oWvrn">Mathematical descriptions of opacity</a></li></ul>

<h2 id="references">References</h2>

<ol>
<li id="fn:1">IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "Absorbance". doi:10.1351/goldbook.A00028 <a href="/wiki/International_Union_of_Pure_and_Applied_Chemistry" target="_blank">/wiki/International_Union_of_Pure_and_Applied_Chemistry</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></li>
<li id="fn:2">IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "decadic absorbance". doi:10.1351/goldbook.D01536 <a href="/wiki/International_Union_of_Pure_and_Applied_Chemistry" target="_blank">/wiki/International_Union_of_Pure_and_Applied_Chemistry</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></li>
<li id="fn:3">Bertie, John E. (2006). "Glossary of Terms used in Vibrational Spectroscopy". In Griffiths, Peter R (ed.). Handbook of Vibrational Spectroscopy. doi:10.1002/0470027320.s8401. ISBN 0471988472. <a href="0471988472" target="_blank">0471988472</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></li>
<li id="fn:4">Van de Hulst, H. C. (1957). Light Scattering by Small Particles. New York: John Wiley and Sons. ISBN 9780486642284. {{cite book}}: ISBN / Date incompatibility (help) <a href="9780486642284" target="_blank">9780486642284</a> <a href="#fnref:4" class="footnote-back-ref">↩</a></li>
<li id="fn:5">IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "decadic absorbance". doi:10.1351/goldbook.D01536 <a href="/wiki/International_Union_of_Pure_and_Applied_Chemistry" target="_blank">/wiki/International_Union_of_Pure_and_Applied_Chemistry</a> <a href="#fnref:5" class="footnote-back-ref">↩</a></li>
<li id="fn:6">Bertie, John E. (2006). "Glossary of Terms used in Vibrational Spectroscopy". In Griffiths, Peter R (ed.). Handbook of Vibrational Spectroscopy. doi:10.1002/0470027320.s8401. ISBN 0471988472. <a href="0471988472" target="_blank">0471988472</a> <a href="#fnref:6" class="footnote-back-ref">↩</a></li>
<li id="fn:7">Dahm, Donald; Dahm, Kevin (2007). Interpreting Diffuse Reflectance and Transmittance: A Theoretical Introduction to Absorption Spectroscopy of Scattering Materials. doi:10.1255/978-1-901019-05-6. ISBN 9781901019056. <a href="9781901019056" target="_blank">9781901019056</a> <a href="#fnref:7" class="footnote-back-ref">↩</a></li>
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<li id="fn:9">GE Health Care (2015). "ÄKTA Laboratory-Scale Chromatography Systems - Instrument Management Handbook". Uppsala: GE Healthcare Bio-Sciences AB. Archived from the original on 2020-03-15. <a href="https://web.archive.org/web/20200315013424/https://cdn.gelifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&destinationid=10016&assetid=16189" target="_blank">https://web.archive.org/web/20200315013424/https://cdn.gelifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&destinationid=10016&assetid=16189</a> <a href="#fnref:9" class="footnote-back-ref">↩</a></li>
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<li id="fn:13">Reusch, William. "Visible and Ultraviolet Spectroscopy". Retrieved 2014-10-29. <a href="https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/Spectrpy/UV-Vis/spectrum.htm" target="_blank">https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/Spectrpy/UV-Vis/spectrum.htm</a> <a href="#fnref:13" class="footnote-back-ref">↩</a></li>
<li id="fn:14">Reusch, William. "Empirical Rules for Absorption Wavelengths of Conjugated Systems". Retrieved 2014-10-29. <a href="https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/Spectrpy/UV-Vis/uvspec.htm#uv1" target="_blank">https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/Spectrpy/UV-Vis/uvspec.htm#uv1</a> <a href="#fnref:14" class="footnote-back-ref">↩</a></li>
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</ol>

Absorbance open-in-new

Absorbance