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RNA-dependent RNA polymerase
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<h2 id="history">History</h2> <p>Viral RdRps were discovered in the early 1960s from studies on <a href="/facts/Mengovirus/IeRsFnCt">mengovirus</a> and <a href="/facts/Polio_virus/M7vyrYvS">polio virus</a> when it was observed that these viruses were not sensitive to <a href="/facts/Actinomycin_D/HvkQHVDc">actinomycin D</a>, a drug that inhibits cellular DNA-directed RNA synthesis. This lack of sensitivity suggested the action of a virus-specific enzyme that could copy RNA from an RNA template.<a class="footnote-ref" id="fnref:3" href="#fn:3"><sup>3</sup></a> </p> <h2 id="distribution">Distribution</h2> <p>RdRps are highly <a href="/facts/Conserved_sequence/YAxy2Xa2">conserved</a> in viruses and are related to <a href="/facts/Telomerase/tUtWedEg">telomerase</a>, though the reason for this was an ongoing question as of 2009.<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> The similarity led to speculation that viral RdRps are ancestral to human telomerase.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a> </p><p>The most famous example of RdRp is in the <a href="/facts/Poliovirus/M7vyrYvS">polio virus</a>. The viral genome is composed of RNA, which enters the cell through receptor-mediated <a href="/facts/Endocytosis/NJjChLNj">endocytosis</a>. From there, the RNA acts as a template for complementary RNA synthesis. The complementary strand acts as a template for the production of new viral genomes that are packaged and released from the cell ready to infect more host cells. The advantage of this method of replication is that no DNA stage complicates replication. The disadvantage is that no 'back-up' DNA copy is available.<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> </p><p>Many RdRps associate tightly with membranes making them difficult to study. The best-known RdRps are polioviral 3Dpol, <a href="/facts/Vesicular_stomatitis_virus/dud7fV3z">vesicular stomatitis virus</a> L,<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a> and <a href="/facts/Hepatitis_C_virus/DFUwAzlB">hepatitis C virus</a> <a href="/facts/NS5B/QupCdRUa">NS5B</a> protein. </p><p>Many eukaryotes have RdRps that are involved in <a href="/facts/RNA_interference/oJRWBXJH">RNA interference</a>: these amplify <a href="/facts/MicroRNA/UjqbYYpe">microRNAs</a> and <a href="/facts/Small_temporal_RNA/ABa4Rbwp">small temporal RNAs</a> and produce <a href="/facts/Double-stranded_RNA/HxPeNfNj">double-stranded RNA</a> using <a href="/facts/Small_interfering_RNA/891ytkD9">small interfering RNAs</a> as primers.<a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a> These RdRps are used in the defense mechanisms and can be appropriated by RNA viruses.<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a> Their evolutionary history predates the divergence of major eukaryotic groups.<a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a> </p> <h2 id="replication">Replication</h2> <p>RdRp differs from <a href="/facts/DNA_dependent_RNA_polymerase/gHd0ET9R">DNA dependent RNA polymerase</a> as it catalyzes RNA synthesis of strands complementary to a given RNA template. The RNA replication process is a four-step mechanism: </p> <ul><li><a href="/facts/Nucleoside_triphosphate/cmhLQj6s">Nucleoside triphosphate</a> (NTP) binding – initially, the RdRp presents with a vacant active site in which an NTP binds, complementary to the corresponding nucleotide on the template strand. Correct NTP binding causes the RdRp to undergo a conformational change.<a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a></li> <li>Active site closure – the conformational change, initiated by the correct NTP binding, results in the restriction of active site access and produces a catalytically competent state.<a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a></li> <li><a href="/facts/Phosphodiester_bond/fCjTAnxa">Phosphodiester bond</a> formation – two Mg2+ ions are present in the catalytically active state and arrange themselves around the newly synthesized RNA chain such that the substrate NTP undergoes a phosphatidyl transfer and forms a phosphodiester bond with the new chain.<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a> Without the use of these Mg2+ ions, the active site is no longer catalytically stable and the RdRp complex changes to an open conformation.<a class="footnote-ref" id="fnref:14" href="#fn:14"><sup>14</sup></a></li> <li>Translocation – once the active site is open, the RNA template strand moves by one position through the RdRp protein complex and continues chain elongation by binding a new NTP, unless otherwise specified by the template.<a class="footnote-ref" id="fnref:15" href="#fn:15"><sup>15</sup></a></li></ul> <p>RNA synthesis can be performed by a <a href="/facts/Primer_(molecular_biology)/I3fBfwte">primer</a>-independent (<i>de novo</i>) or a primer-dependent mechanism that utilizes a <a href="/facts/VPg/nXGMYSBh">viral protein genome-linked</a> (VPg) primer.<a class="footnote-ref" id="fnref:16" href="#fn:16"><sup>16</sup></a> The <i>de novo</i> initiation consists in the addition of a NTP to the 3'-OH of the first initiating NTP.<a class="footnote-ref" id="fnref:17" href="#fn:17"><sup>17</sup></a> During the following elongation phase, this nucleotidyl transfer reaction is repeated with subsequent NTPs to generate the complementary RNA product. Termination of the nascent RNA chain produced by RdRp is not completely known, however, RdRp termination is sequence-independent.<a class="footnote-ref" id="fnref:18" href="#fn:18"><sup>18</sup></a> </p><p>One major drawback of RNA-dependent RNA polymerase replication is the transcription error rate.<a class="footnote-ref" id="fnref:19" href="#fn:19"><sup>19</sup></a> RdRps lack fidelity on the order of 104 nucleotides, which is thought to be a direct result of inadequate proofreading.<a class="footnote-ref" id="fnref:20" href="#fn:20"><sup>20</sup></a> This variation rate is favored in viral genomes as it allows for the pathogen to overcome host defenses trying to avoid infection, allowing for evolutionary growth.<a class="footnote-ref" id="fnref:21" href="#fn:21"><sup>21</sup></a> </p> <h2 id="structure">Structure</h2> <p>Viral/prokaryotic RdRp, along with many single-subunit DdRp, employ a fold whose organization has been linked to the shape of a right hand with three subdomains termed fingers, palm, and thumb.<a class="footnote-ref" id="fnref:22" href="#fn:22"><sup>22</sup></a> Only the palm subdomain, composed of a four-stranded <a href="/facts/Antiparallel_(biochemistry)/IMsRKh5G">antiparallel</a> <a href="/facts/Beta_sheet/B6mwwR6H">beta sheet</a> with two <a href="/facts/Alpha_helix/7CESPIYJ">alpha helices</a>, is well conserved. In RdRp, the palm subdomain comprises three well-conserved <a href="/facts/Sequence_motif/RtcoysBD">motifs</a> (A, B, and C). Motif A (D-x(4,5)-D) and motif C (GDD) are spatially juxtaposed; the <a href="/facts/Aspartic_acid/9DFSFPa1">aspartic acid</a> residues of these motifs are implied in the binding of Mg2+ and/or Mn2+. The <a href="/facts/Asparagine/KYIft5Jd">asparagine</a> residue of motif B is involved in selection of <a href="/facts/Ribonucleoside/8lAokLM4">ribonucleoside</a> triphosphates over dNTPs and, thus, determines whether RNA rather than DNA is synthesized.<a class="footnote-ref" id="fnref:23" href="#fn:23"><sup>23</sup></a> The domain organization<a class="footnote-ref" id="fnref:24" href="#fn:24"><sup>24</sup></a> and the 3D structure of the catalytic centre of a wide range of RdRps, even those with a low overall sequence homology, are conserved. The catalytic center is formed by several motifs containing conserved amino acid residues. </p><p>Eukaryotic <a href="/facts/RNA_interference/oJRWBXJH">RNA interference</a> requires a cellular RdRp (c RdRp). Unlike the "hand" polymerases, they resemble simplified multi-subunit DdRPs, specifically in the catalytic β/β' subunits, in that they use two sets of double-psi β-barrels in the active site. QDE1 (<a href="https://www.uniprot.org/uniprot/Q9Y7G6">Q9Y7G6</a>) in <i><a href="/facts/Neurospora_crassa/zI0Ub5p6">Neurospora crassa</a></i>, which has both barrels in the same chain,<a class="footnote-ref" id="fnref:25" href="#fn:25"><sup>25</sup></a> is an example of such a c RdRp enzyme.<a class="footnote-ref" id="fnref:26" href="#fn:26"><sup>26</sup></a> <a href="/facts/Bacteriophage/L2Ll0T3q">Bacteriophage</a> homologs of c RdRp, including the similarly single-chain DdRp yonO (<a href="https://www.uniprot.org/uniprot/O31945">O31945</a>), appear to be closer to c RdRps than DdRPs are.<a class="footnote-ref" id="fnref:27" href="#fn:27"><sup>27</sup></a><a class="footnote-ref" id="fnref:28" href="#fn:28"><sup>28</sup></a> </p> <table><tbody><tr><td></td><td></td><td></td></tr></tbody></table> <h2 id="viruses">Viruses</h2> <p>Four superfamilies of viruses cover all RNA-containing viruses with no DNA stage: </p> <ul><li>Viruses containing positive-strand RNA or double-strand RNA, except <a href="/facts/Retrovirus/GWAx3C3o">retroviruses</a> and <i><a href="/facts/Birnaviridae/8SnAzaaH">Birnaviridae</a></i> <ul><li>All positive-strand RNA eukaryotic viruses with no DNA stage, such as <i><a href="/facts/Coronaviridae/NVjnbxYV">Coronaviridae</a></i></li> <li>All RNA-containing <a href="/facts/Bacteriophage/L2Ll0T3q">bacteriophages</a>; the two families of RNA-containing bacteriophages are <i><a href="/facts/Fiersviridae/WfUkMYdt">Fiersviridae</a></i> (positive ssRNA phages) and <i><a href="/facts/Cystovirus/YRFVMMmj">Cystoviridae</a></i> (dsRNA phages)</li> <li>dsRNA virus family <i><a href="/facts/Reoviridae/zDHSs9bK">Reoviridae</a></i>, <i><a href="/facts/Totiviridae/RoJWat6G">Totiviridae</a></i>, <i><a href="/facts/Hypoviridae/V7v6yACL">Hypoviridae</a></i>, <i><a href="/facts/Partitiviridae/rJfkLb8k">Partitiviridae</a></i></li></ul></li> <li><i><a href="/facts/Mononegavirales/nxfeEBrg">Mononegavirales</a></i> (negative-strand RNA viruses with non-segmented genomes; <a href="/facts/InterPro/pKwNm8t7">InterPro</a>: <i><a href="https://www.ebi.ac.uk/interpro/entry/IPR016269">IPR016269</a></i>)</li> <li>Negative-strand RNA viruses with segmented genomes (<a href="/facts/InterPro/pKwNm8t7">InterPro</a>: <i><a href="https://www.ebi.ac.uk/interpro/entry/IPR007099">IPR007099</a></i>), such as <a href="/facts/Orthomyxoviridae/3gqqgCMp">orthomyxoviruses</a> and <a href="/facts/Bunyavirales/vBJSpVdc">bunyaviruses</a></li> <li>dsRNA virus family <i><a href="/facts/Birnaviridae/8SnAzaaH">Birnaviridae</a></i> (<a href="/facts/InterPro/pKwNm8t7">InterPro</a>: <i><a href="https://www.ebi.ac.uk/interpro/entry/IPR007100">IPR007100</a></i>)</li></ul> <p><a href="/facts/Flavivirus/J1DqLnX8">Flaviviruses</a> produce a polyprotein from the ssRNA genome. The <a href="/facts/Polyprotein/GtQdv6J7">polyprotein</a> is cleaved to a number of products, one of which is NS5, an RdRp. It possesses short regions and motifs homologous to other RdRps.<a class="footnote-ref" id="fnref:29" href="#fn:29"><sup>29</sup></a> </p><p>RNA replicase found in positive-strand ssRNA viruses are related to each other, forming three large superfamilies.<a class="footnote-ref" id="fnref:30" href="#fn:30"><sup>30</sup></a> Birnaviral RNA replicase is unique in that it lacks motif C (GDD) in the palm.<a class="footnote-ref" id="fnref:31" href="#fn:31"><sup>31</sup></a> Mononegaviral RdRp (PDB 5A22) has been automatically classified as similar to (+)−ssRNA RdRps, specifically one from <i><a href="/facts/Pestivirus/yMduVJQM">Pestivirus</a></i> and one from <i><a href="/facts/Leviviridae/WfUkMYdt">Leviviridae</a></i>.<a class="footnote-ref" id="fnref:32" href="#fn:32"><sup>32</sup></a> Bunyaviral RdRp monomer (PDB 5AMQ) resembles the <a href="/facts/Protein_trimer/hCMCasTN">heterotrimeric</a> complex of Orthomyxoviral (Influenza; PDB 4WSB) RdRp.<a class="footnote-ref" id="fnref:33" href="#fn:33"><sup>33</sup></a> </p><p>Since it is a protein universal to RNA-containing viruses, RdRp is a useful marker for understanding their evolution.<a class="footnote-ref" id="fnref:34" href="#fn:34"><sup>34</sup></a><a class="footnote-ref" id="fnref:35" href="#fn:35"><sup>35</sup></a> </p> <h3>Recombination</h3> <p>When replicating its <a href="/facts/Positive-sense_single-stranded_RNA_virus/tEqWffsl">(+)ssRNA genome</a>, the <a href="/facts/Poliovirus/M7vyrYvS">poliovirus</a> RdRp is able to carry out <a href="/facts/Genetic_recombination/uocNH0m7">recombination</a>. Recombination appears to occur by a copy choice mechanism in which the RdRp switches (+)ssRNA templates during negative strand synthesis.<a class="footnote-ref" id="fnref:36" href="#fn:36"><sup>36</sup></a> Recombination frequency is determined in part by the fidelity of RdRp replication.<a class="footnote-ref" id="fnref:37" href="#fn:37"><sup>37</sup></a> RdRp variants with high replication fidelity show reduced recombination, and low fidelity RdRps exhibit increased recombination.<a class="footnote-ref" id="fnref:38" href="#fn:38"><sup>38</sup></a> Recombination by RdRp strand switching occurs frequently during replication in the (+)ssRNA plant <a href="/facts/Carmovirus/fnHULh78">carmoviruses</a> and <a href="/facts/Tombusvirus/QsVCV9lm">tombusviruses</a>.<a class="footnote-ref" id="fnref:39" href="#fn:39"><sup>39</sup></a> </p> <h3>Intragenic complementation</h3> <p><a href="/facts/Murine_respirovirus/sP7IkwR9">Sendai virus</a> (family <i>Paramyxoviridae</i>) has a linear, single-stranded, negative-sense, nonsegmented RNA genome. The viral RdRp consists of two virus-encoded subunits, a smaller one P and a larger one L. Testing different inactive RdRp mutants with defects throughout the length of the L subunit in pairwise combinations, restoration of viral RNA synthesis was observed in some combinations.<a class="footnote-ref" id="fnref:40" href="#fn:40"><sup>40</sup></a> This positive L–L interaction is referred to as <a href="/facts/Complementation_(genetics)/4YJL6RGW">intragenic complementation</a> and indicates that the L protein is an <a href="/facts/Oligomer/v7oDY8zu">oligomer</a> in the viral RNA polymerase complex. </p> <h2 id="drug-therapies">Drug therapies</h2> <ul><li>RdRps can be used as drug targets for viral pathogens as their function is not necessary for eukaryotic survival. By inhibiting RdRp function, new RNAs cannot be replicated from an RNA template strand, however, DNA-dependent RNA polymerase remains functional.</li> <li>Some antiviral drugs against <a href="/facts/Hepatitis_C/kwb7IIht">Hepatitis C</a> and <a href="/facts/COVID-19/YsUgvsaX">COVID-19</a> specifically target RdRp. These include <a href="/facts/Sofosbuvir/muvEeSWA">Sofosbuvir</a> and <a href="/facts/Ribavirin/4NQT4Vuu">Ribavirin</a> against Hepatitis C<a class="footnote-ref" id="fnref:41" href="#fn:41"><sup>41</sup></a> and <a href="/facts/Remdesivir/3Nk67cpG">remdesivir</a>, an <a href="/facts/Food_and_Drug_Administration/rCEnh9WB">FDA</a> approved drug against COVID-19</li> <li><a href="/facts/GS-441524/RuQQTLRg">GS-441524</a> triphosphate is a substrate for RdRp, but not mammalian polymerases. It results in premature chain termination and inhibition of viral replication. GS-441524 triphosphate is the biologically active form of remdesivir. Remdesivir is classified as a <a href="/facts/Nucleoside_analogue/DEr9cQYD">nucleotide analog</a> that inhibits RdRp function by covalently binding to and interrupting termination of the nascent RNA through early or delayed termination or preventing further elongation of the RNA polynucleotide.<a class="footnote-ref" id="fnref:42" href="#fn:42"><sup>42</sup></a><a class="footnote-ref" id="fnref:43" href="#fn:43"><sup>43</sup></a> This early termination leads to nonfunctional RNA that gets degraded through normal cellular processes.</li></ul> <h2 id="rna-interference">RNA interference</h2> <p>The use of RdRp plays a major role in <a href="/facts/RNA_interference/oJRWBXJH">RNA interference</a> in eukaryotes, a process used to silence gene expression via small interfering RNAs (<a href="/facts/Small_interfering_RNA/891ytkD9">siRNAs</a>) binding to mRNA rendering them inactive.<a class="footnote-ref" id="fnref:44" href="#fn:44"><sup>44</sup></a> Eukaryotic RdRp becomes active in the presence of dsRNA, and is less widely distributed than other RNAi components as it lost in some animals, though still found in <i><a href="/facts/Caenorhabditis_elegans/XJVJzPn2">C. elegans</a>,</i> <i><a href="/facts/Paramecium/s82lDhHe">P. tetraurelia</a>,</i><a class="footnote-ref" id="fnref:45" href="#fn:45"><sup>45</sup></a> and <a href="/facts/Plants/X5BMDMvS">plants</a>.<a class="footnote-ref" id="fnref:46" href="#fn:46"><sup>46</sup></a> This presence of dsRNA triggers the activation of RdRp and RNAi processes by priming the initiation of RNA transcription through the introduction of siRNAs.<a class="footnote-ref" id="fnref:47" href="#fn:47"><sup>47</sup></a> In <i>C. elegans</i>, siRNAs are integrated into the RNA-induced silencing complex, <a href="/facts/RNA-induced_silencing_complex/jgSHI5vB">RISC</a>, which works alongside mRNAs targeted for interference to recruit more RdRps to synthesize more secondary siRNAs and repress gene expression.<a class="footnote-ref" id="fnref:48" href="#fn:48"><sup>48</sup></a> </p> <h2 id="see-also">See also</h2> <ul><li><a href="/facts/Spiegelman%2527s_Monster/tJTwS4wM">Spiegelman's Monster</a></li> <li><a href="/facts/NS5B_inhibitor/v4egH900">NS5B inhibitor</a></li></ul> <h2 id="notes">Notes</h2> <h2 id="external-links">External links</h2> <ul><li><a href="https://meshb.nlm.nih.gov/record/ui?name=RNA+Replicase">RNA+Replicase</a> at the U.S. National Library of Medicine <a href="/facts/Medical_Subject_Headings/C57g2JuF">Medical Subject Headings</a> (MeSH)</li> <li><a href="/facts/Enzyme_Commission_number/Y5GNLRKX">EC</a> <a href="https://enzyme.expasy.org/EC/2.7.7.48">2.7.7.48</a></li></ul> This article incorporates text from the public domain <a href="/facts/Pfam/BKJa293w">Pfam</a> and <a href="/facts/InterPro/pKwNm8t7">InterPro</a>: <a href="https://www.ebi.ac.uk/interpro/entry/IPR000208">IPR000208</a> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Koonin EV, Gorbalenya AE, Chumakov KM (July 1989). 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