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Dechloromonas agitata strain CKB
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<h2 id="biological-perchlorate-reduction-by-strain-ckb">Biological perchlorate reduction by strain CKB</h2> <p>Strain CKB can reduce chlorate and perchlorate (collectively known as (per)chlorate) completely to <a href="/facts/Chloride/m9NUaUDR">chloride</a>. The first step of the pathway involves the reduction of (per)chlorate to <a href="/facts/Chlorite/EYtQI4wC">chlorite</a> via the <a href="/facts/Perchlorate_reductase/tPRS1p5z">perchlorate reductase</a> (Pcr) <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a>. Chlorite is then dismutated to molecular oxygen and chloride by the <a href="/facts/Chlorite_dismutase/oIxtdCEJ">chlorite dismutase</a> (Cld) enzyme. The produced oxygen can then be reduced to water by action of the enzyme <a href="/facts/Cytochrome_oxidase/U67QVCv8">cytochrome oxidase</a>. The Cld enzyme, encoded by an 834 bp <a href="/facts/Gene/e1swwPY6">gene</a>, is 277 amino acids long and includes a 26 amino acid leader peptide. Though expressed at basal levels under aerobic conditions, the cld gene was <a href="/facts/Transcription_(genetics)/bDgp9HDN">transcriptionally</a> upregulated when strain CKB cells were grown in (per)chlorate. The Cld enzyme is of particular interest not only because it protects cells from toxic chlorite, but also because it is one of only a handful of systems capable of producing molecular oxygen. </p> <ul><li>Royce A. Bruce; Laurie A. Achenbach; John D. Coates (1999). "Reduction of (per)chlorate by a novel organism isolated from paper mill waste". <i>Environmental Microbiology</i>. 1 (4): 319–329. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1046%2Fj.1462-2920.1999.00042.x">10.1046/j.1462-2920.1999.00042.x</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/11207750">11207750</a>.</li> <li>Kelly S. Bender; Susan M. O’Connor; Romy Chakraborty; John D. Coates; Laurie A. Achenbach (2002). <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126438">"Sequencing and Transcriptional Analysis of the Chlorite Dismutase Gene of Dechloromonas agitata and Its Use as a Metabolic Probe"</a>. <i>Applied and Environmental Microbiology</i>. 68 (10): 4820–4826. <a href="/facts/Doi_(identifier)/muM9Etpq">doi</a>:<a href="https://doi.org/10.1128%2FAEM.68.10.4820-4826.2002">10.1128/AEM.68.10.4820-4826.2002</a>. <a href="/facts/PMC_(identifier)/dX1zMt71">PMC</a> <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126438">126438</a>. <a href="/facts/PMID_(identifier)/JlHAvMHt">PMID</a> <a href="https://pubmed.ncbi.nlm.nih.gov/12324326">12324326</a>.</li></ul> <h2 id="external-links">External links</h2> <ul><li><a href="http://bacdive.dsmz.de/index.php?search=13894&submit=Search">Type strain of <i>Dechloromonas agitata</i> at Bac<i>Dive</i> - the Bacterial Diversity Metadatabase</a></li></ul>