The process is thought to involve two interrelated processes, occurring in the germinal centers of the secondary lymphoid organs:
Like the natural prototype, the in vitro affinity maturation is based on the principles of mutation and selection. The in vitro affinity maturation has successfully been used to optimize antibodies, antibody fragments or other peptide molecules like antibody mimetics. Random mutations inside the CDRs are introduced using radiation, chemical mutagens or error-prone PCR. In addition, the genetic diversity can be increased by chain shuffling. Two or three rounds of mutation and selection using display methods like phage display usually results in antibody fragments with affinities in the low nanomolar range.5
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Roskos L.; Klakamp S.; Liang M.; Arends R.; Green L. (2007). Stefan Dübel (ed.). Handbook of Therapeutic Antibodies. Weinheim: Wiley-VCH. pp. 145–169. ISBN 978-3-527-31453-9. 978-3-527-31453-9 ↩