Base calling

<h2 id="base-calling-accuracy">Base calling accuracy</h2>
<p>Base calling can be assessed by two metrics, read accuracy and consensus accuracy. Read accuracy refers to the called base's accuracy to a known reference. Consensus accuracy refers to how accurate a consensus sequence is compared to overlapping reads from the same <a href="/facts/Genetic_locus/JcIT8wLm">genetic locus</a>. <a class="footnote-ref" id="fnref:3" href="#fn:3"><sup>3</sup></a>
</p>

<h2 id="references">References</h2>

<ol>
<li id="fn:1"><p>Richterich, Peter (1998-03-01). "Estimation of Errors in "Raw" DNA Sequences: A Validation Study". Genome Research. 8 (3). Cold Spring Harbor Laboratory: 251–259. doi:10.1101/gr.8.3.251. ISSN 1088-9051. PMC 310698. PMID 9521928. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC310698" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC310698</a> <a href="#fnref:1" class="footnote-back-ref">↩</a></p></li>
<li id="fn:2"><p>Wick, Ryan R.; Judd, Louise M.; Holt, Kathryn E. (2019-06-24). "Performance of neural network basecalling tools for Oxford Nanopore sequencing". Genome Biology. 20 (1). Springer Science and Business Media LLC: 129. doi:10.1186/s13059-019-1727-y. ISSN 1474-760X. PMC 6591954. PMID 31234903. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591954" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591954</a> <a href="#fnref:2" class="footnote-back-ref">↩</a></p></li>
<li id="fn:3"><p>Wick, Ryan R.; Judd, Louise M.; Holt, Kathryn E. (2019-06-24). "Performance of neural network basecalling tools for Oxford Nanopore sequencing". Genome Biology. 20 (1). Springer Science and Business Media LLC: 129. doi:10.1186/s13059-019-1727-y. ISSN 1474-760X. PMC 6591954. PMID 31234903. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591954" target="_blank">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591954</a> <a href="#fnref:3" class="footnote-back-ref">↩</a></p></li>
</ol>

Base calling open-in-new

Base calling