When Griffith was in elementary school, she joined the Sugar Ray Robinson Organization, running in track meets on weekends. She won the Jesse Owens National Youth Games two years in a row, at the ages of 14 and 15. Griffith ran track at Jordan High School in Los Angeles.
Showing an early interest in fashion, Griffith persuaded the members of the track team to wear tights with their uniforms. As a high school senior in 1978, she finished sixth at the CIF California State Meet behind future teammates Alice Brown and Pam Marshall. By the time she graduated from Jordan High School in 1978, she had set high-school records in sprinting and long jump.
Brown, Bolden, and Griffith qualified for the 100-meter final at the trials for the 1980 Summer Olympics (with Brown winning and Griffith finishing last in the final). Griffith also ran the 200 meters, narrowly finishing fourth, a foot out of a qualifying position. However, the U.S. Government had already decided to boycott those Olympic Games mooting those results. In 1983, Griffith graduated from UCLA with her bachelor's degree in psychology.
After the 1984 Olympic Games, she spent less time running. Griffith continued to run part-time, winning the 100-meter IAAF Grand Prix Final with the time of 11.00 seconds. She did not compete at the 1985 U.S. National Championship. That same year, she returned to working at a bank and styled hair and nails in her spare time. She married Al Joyner, the Olympic triple jump champion of 1984, in 1987.
The 100-meter record was by far the largest improvement in the world record time since the advent of electronic timing, and still stands. This extraordinary result raised the possibility of a technical malfunction with the wind gauge which read at 0.0 m/s - a reading at odds with the windy conditions on the day, with high wind speeds being recorded in all other sprints before and after this race as well as the parallel long jump runway at the time of the Griffith Joyner performance. All scientific studies commissioned by the IAAF and independent organizations have since found there was an illegal tailwind of between 5 m/s – 7 m/s at the time. The IAAF has not annulled the result, but since 1997 the International Athletics Annual of the Association of Track and Field Statisticians has listed it as "probably strongly wind assisted, but recognized as a world record." The fastest non-wind-assisted performance would then be Griffith Joyner's 10.61s in the final the next day. This mark was equaled by Elaine Thompson-Herah in the 2020 Olympic Final before being surpassed by Thompson-Herah at the post-Olympics Eugene Diamond League meeting in August 2021. Thompson-Herah clocked 10.54 seconds, officially the second fastest time in women's 100 m history.
Following the Olympic trials, in late July 1988, Griffith Joyner left coach Kersee saying she wanted a coach able to provide more personal attention. Another contributing factor was her unhappiness with the lack of sponsorship and endorsement opportunities. In addition to being her coach, Kersee was Griffith Joyner's manager, as he required all the athletes he coached to use his management services too. Griffith Joyner's decision to sign with personal manager Gordon Baskin therefore necessitated the coaching change. She left UCLA for UC Irvine with her husband serving as full-time coach.
By then known to the world as "Flo-Jo", Griffith Joyner was the big favorite for the titles in the sprint events at the 1988 Summer Olympics. In the 100-meter final, she ran a 10.54, beating her nearest rival to the world record, Evelyn Ashford, by 0.30 seconds. In the 200 meter semifinal, she set the world record of 21.56 seconds and then broke this record by 0.22 seconds in winning the final with a time of 21.34 seconds. Like her 100-meter world record, this mark still stands.
At the same Olympics, Griffith Joyner also ran with the 4 × 100 m relay and the 4 × 400 m relay teams. Her team won the 4 × 100 m relay and finished second in the 4 × 400 m relay. This was her first internationally rated 4 × 400 m relay. She left the games having won four Olympic medals, three gold and one silver. At the time, her medal haul was the second most for female track and field athlete in history, behind only Fanny Blankers-Koen who won four gold medals in 1948.
In February 1989, Griffith Joyner announced her retirement from racing. She cited her new business opportunities outside of sprinting. The month after announcing her retirement, she was selected as the winner of the James E. Sullivan Award of 1988 as the top amateur athlete in the United States.
Griffith Joyner's success at the 1988 Olympics led to new opportunities. In the weeks following the Olympics, she earned millions of dollars from endorsement deals, primarily in Japan. She also signed a deal with toy maker LJN Toys for a Barbie-like doll in her likeness.
Beyond her running prowess, Griffith Joyner was known for her bold fashion choices. She appeared at the World Championships in 1987 in Rome wearing a hooded speed skating body suit. In April 1988, she started wearing a running suit with the right leg of the suit extending to the ankle and the left leg of the suit cut off, a style she called the "one-legger". The running suits had bold colors such as lime green and purple with white bikini bottoms and were embellished with lightning bolts.
Her nails also garnered attention for their length and designs. Her nails were four inches long with tiger stripes at the 1988 Olympic trials before switching to fuchsia. For the Olympic games themselves, she had six inch nails painted red, white, blue, and gold. Although many sprinters avoided accessories which might slow them down, Griffith Joyner kept her hair long and wore jewelry while competing. She designed many of her outfits herself and preferred looks which were not conventional.
After her record-shattering performances at the 1988 U.S. Olympic Trials, she became an object of suspicion when she arrived at the 1988 Olympic Games in Seoul. Athletes, including Joaquim Cruz and Ben Johnson, expressed disbelief over Griffith Joyner's dramatic improvement over a short period of time. Before the 1988 track and field season, her best time in the 100-meter sprint was 10.96 seconds (set in 1987). In 1988, she improved that by 0.47 seconds.
Her best before 1988 at 200 meters was 21.96 seconds (also set in 1987). In 1988, she improved that by 0.62 seconds to 21.34 seconds, another time that has not been approached. Griffith Joyner attributed the change in her physique to new health programs. Al Joyner replaced Bob Kersee as her coach, and he changed her training program to include more lower body strength training exercises such as squats and lunges.
Neither Robinson nor Lewis provided evidence for their allegations, and Robinson was shunned by the athletics community, leading to the premature end of his career. After the 1988 Olympics, Griffith Joyner retired from competitive track and field, a year before the introduction of mandatory random drug testing in 1989. She was repeatedly tested during competition and passed every test.
Griffith's nickname among her family was "Dee Dee". She was briefly engaged to hurdler Greg Foster. In 1987, Griffith married 1984 Olympic triple-jump champion Al Joyner, whom Griffith had first met at the 1980 Olympic Trials. Through her marriage to Joyner she was sister-in-law to track and field athlete Jackie Joyner-Kersee. Griffith and Joyner had one daughter together, Mary Ruth Joyner, born November 15, 1990.
On September 21, 1998, Griffith Joyner died in her sleep at home in the Canyon Crest neighborhood of Mission Viejo, California, at the age of 38. The unexpected death was investigated by the Orange County Sheriff-Coroner's office, which on September 22 declared the cause of death to be suffocation during a severe epileptic seizure.
To date, her 1988 200 m world and Olympic record (21.34) as well as her 100 m world record (10.49) still stand, making her the only female athlete to hold simultaneous records. Her 100 m Olympic record (10.62) was beaten in 2021 at the 2020 Summer Olympics in Tokyo by Elaine Thompson-Herah (10.61).
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It is widely believed that the anemometer was faulty for the race in which Griffith Joyner set this record.[70] A 1995 report commissioned by the IAAF estimated the true wind speed was between +5.0 m/s and +7.0 m/s, rather than the 0.0 recorded.[70] If this time, recorded in the quarter-final of the 1988 U.S. Olympic trials, were excluded, the world record (until the 10.54 recorded by Elaine Thompson-Herah on August 21, 2021) would have been 10.61 s, also by Griffith Joyner, recorded the next day at the same venue in the final.[70][71]