Replication factor C

The replication factor C, or RFC, is a five-subunit <a href="/facts/Protein_complex/GW11Ee34">protein complex</a> that is required for <a href="/facts/DNA_replication/wrU6RuR3">DNA replication</a>.
The <a href="/facts/Protein_subunit/HhuET9UP">subunits</a> of this <a href="/facts/Heteropentamer/JUdMmxlg">heteropentamer</a> are named <a href="/facts/RFC1/PF058v63">Rfc1</a>, <a href="/facts/RFC2/274ueYDx">Rfc2</a>, <a href="/facts/RFC3/4SQacnEC">Rfc3</a>, <a href="/facts/RFC4/Fda3RNtq">Rfc4</a>, and <a href="/facts/RFC5/nEv5Mmwv">Rfc5</a> in <a href="/facts/Saccharomyces_cerevisiae/sX9MGFrz">Saccharomyces cerevisiae</a>. RFC is used in eukaryotic replication as a clamp loader, similar to the <a href="/facts/DNA_polymerase_III_subunit_gamma%2ftau/8DeVW9EY">γ Complex</a> in <a href="/facts/Escherichia_coli/nR4Gvl56">Escherichia coli</a>. Its role as a clamp loader involves catalyzing the loading of <a href="/facts/Proliferating_cell_nuclear_antigen/KIBbmpXz">PCNA</a> onto DNA. It binds to the <a href="/facts/3%2527_end/TIAXEQmY">3' end</a> of the <a href="/facts/Deoxyribonucleic_acid/nB89Iauz">DNA</a> and uses <a href="/facts/Adenosine_triphosphate/nB9bwgcU">ATP</a> to open the ring of PCNA so that it can encircle the DNA. ATP hydrolysis causes the release of RFC, with concomitant clamp loading onto DNA. For DNA polymerase, RFC serves as primer identification. RFC plays an important role in the proliferation, invasion, and progression of various malignant tumors. RFC acts as a tumor suppressor gene.

Replication factor C open-in-new

Replication factor C