Menu
Home
People
Places
Arts
History
Plants & Animals
Science
Life & Culture
Technology
Reference.org
Triple-resonance nuclear magnetic resonance spectroscopy
open-in-new
<p>Triple resonance experiments are a set of multi-dimensional <a href="/facts/Nuclear_magnetic_resonance_spectroscopy/SFKgdthI">nuclear magnetic resonance spectroscopy</a> (NMR) experiments that link three types of <a href="/facts/Atomic_nucleus/orpIm5Xl">atomic nuclei</a>, most typically consisting of 1H, 15N and 13C. These experiments are often used to assign specific resonance signals to specific atoms in an isotopically-enriched protein. The technique was first described in papers by <a href="/facts/Ad_Bax/mr3A3UnL">Ad Bax</a>, Mitsuhiko Ikura and <a href="/facts/Lewis_E._Kay/7YYKSdrq">Lewis Kay</a> in 1990, and further experiments were then added to the suite of experiments. Many of these experiments have since become the standard set of experiments used for sequential assignment of NMR resonances in the determination of <a href="/facts/Nuclear_magnetic_resonance_spectroscopy_of_proteins/lCIXAFzQ">protein structure by NMR</a>. They are now an integral part of solution NMR study of proteins, and they may also be used in <a href="/facts/Solid-state_nuclear_magnetic_resonance/cumWwN7Y">solid-state NMR</a>. </p>