Fed-batch culture

<p>Fed-batch culture is, in the broadest sense,  defined as an operational technique in <a href="/facts/Biotechnological/8eFnI6FB">biotechnological</a> processes where one or more <a href="/facts/Nutrient/34bn4skK">nutrients</a> (substrates) are fed (supplied) to the <a href="/facts/Bioreactor/hkUGIbCy">bioreactor</a> during cultivation and in which the product(s) remain in the bioreactor until the end of the run.  An alternative description of the method is that of a culture in which "a base medium supports initial <a href="/facts/Cell_culture/7Qhu13D9">cell culture</a> and a feed medium is added to prevent nutrient depletion".  It is also a type of semi-batch culture. In some cases, all the nutrients are fed into the bioreactor. The advantage of the fed-batch culture is that one can control concentration of fed-substrate in the culture liquid at arbitrarily desired levels (in many cases, at low levels).
</p><p>Generally speaking, fed-batch culture is superior to conventional batch culture when controlling concentrations of a nutrient (or nutrients) affects the yield or productivity of the desired metabolite.
</p>

Fed-batch culture open-in-new

Fed-batch culture