ChIP-exo

<p>ChIP-exo is a <a href="/facts/Chromatin_immunoprecipitation/Wx5o8hQq">chromatin immunoprecipitation</a> based method for mapping the locations at which a protein of interest (<a href="/facts/Transcription_factor/wro0DPHe">transcription factor</a>) binds to the genome. It is a modification of the <a href="/facts/ChIP-seq/bCxyF70g">ChIP-seq</a> protocol, improving the resolution of binding sites from hundreds of <a href="/facts/Base_pair/xmPpPQ9W">base pairs</a> to almost one base pair. It employs the use of <a href="/facts/Exonucleases/5RezcQvD">exonucleases</a> to degrade strands of the protein-bound DNA in the 5'-3' direction to within a small number of nucleotides of the protein binding site. The nucleotides of the exonuclease-treated ends are determined using some combination of <a href="/facts/DNA_sequencing/hsPsNCQW">DNA sequencing</a>, <a href="/facts/Microarrays/LvJeNodc">microarrays</a>, and <a href="/facts/Polymerase_chain_reaction/G0jWzJy1">PCR</a>. These sequences are then mapped to the genome to identify the locations on the genome at which the protein binds.
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ChIP-exo open-in-new

ChIP-exo