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Bio-layer interferometry
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<p>Bio-layer interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling. Alongside <a href="/facts/Surface_plasmon_resonance/8JKldMUT">Surface Plasmon Resonance</a>, BLI is one of few widely available <a href="/facts/Label-free_quantification/obIr70Io">label-free</a> biosensing technologies, a detection style that yields more information in less time than traditional processes. The technology relies on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor. BLI has significant applications in quantifying binding strength, measuring protein interactions, and identifying properties of reaction kinetics, such as rate constants and reaction rates. </p>