Reduced representation bisulfite sequencing

<p>Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analyzing the genome-wide <a href="/facts/Methylation/EvDMWVW5">methylation</a> profiles on a single nucleotide level.  It combines <a href="/facts/Restriction_enzyme/Mg0VgkCX">restriction enzymes</a> and <a href="/facts/Bisulfite_sequencing/TAXg1DL7">bisulfite sequencing</a> to enrich for areas of the genome with a high <a href="/facts/CpG_site/PbPHokwh">CpG</a> content. Due to the high cost and depth of <a href="/facts/DNA_sequencing/hsPsNCQW">sequencing</a> to analyze methylation status in the entire genome, Meissner et al. developed this technique in 2005 to reduce the amount of nucleotides required to sequence to 1% of the genome.  The fragments that comprise the reduced genome still include the majority of <a href="/facts/Promoter_(genetics)/YDYBzLfg">promoters</a>, as well as regions such as <a href="/facts/Repeated_sequence_(DNA)/hPosWry7">repeated sequences</a> that are difficult to profile using conventional bisulfite sequencing approaches.
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Reduced representation bisulfite sequencing open-in-new

Reduced representation bisulfite sequencing