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Resolution by Proxy
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<p>Resolution by Proxy (ResProx) is a method for assessing the equivalent <a href="/facts/X-ray/wseP79cN">X-ray</a> resolution of <a href="/facts/NMR/pGYAn9Bz">NMR</a>-derived protein structures. ResProx calculates resolution from <a href="/facts/Coordinate/Mtd0q4SP">coordinate</a> data rather than from electron density or other experimental inputs. This makes it possible to calculate the resolution of a structure regardless of how it was solved (<a href="/facts/X-ray/wseP79cN">X-ray</a>, <a href="/facts/NMR/pGYAn9Bz">NMR</a>, EM, modeling, <a href="/facts/Ab_initio_structure_prediction/vsqAt3gb">ab initio prediction</a>). ResProx was originally designed to serve as a simple, single-number evaluation that allows straightforward comparison between the quality/resolution of <a href="/facts/X-ray/wseP79cN">X-ray</a> structures and the quality of a given <a href="/facts/NMR/pGYAn9Bz">NMR</a> structure. However, it can also be used to assess the reliability of an experimentally reported <a href="/facts/X-ray/wseP79cN">X-ray</a> structure resolution, to evaluate <a href="/facts/Protein_structure/BptnTs9X">protein structures</a> solved by unconventional or hybrid means and to identify fraudulent structures deposited in the PDB. ResProx incorporates more than 25 different structural features to determine a single resolution-like value. ResProx values are reported in Angstroms. Tests on thousands of <a href="/facts/X-ray/wseP79cN">X-ray</a> structures show that ResProx values match very closely to resolution values reported by <a href="/facts/X-ray/wseP79cN">X-ray</a> crystallographers. Resolution-by-proxy values can be calculated for newly determined <a href="/facts/Protein_structure/BptnTs9X">protein structures</a> using a freely accessible ResProx web server. This server accepts protein <a href="/facts/Coordinate/Mtd0q4SP">coordinate</a> data (in <a href="/facts/Protein_Data_Bank/oUhq4ABD">PDB</a> format) and generates a resolution estimate (in Angstroms) for that input structure. </p>