ABI Solid Sequencing

SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a <a href="/facts/Single-molecule_sequencing/hsPsNCQW">next-generation DNA sequencing</a> technology developed by <a href="/facts/Life_Technologies_(Thermo_Fisher_Scientific)/QjnbhL8R">Life Technologies</a> and has been commercially available since 2006. This next generation technology generates 108 - 109 small sequence reads at one time. It uses <a href="/facts/2_base_encoding/z1Ctqpuh">2 base encoding</a> to decode the raw data generated by the sequencing platform into sequence data.
This method should not be confused with "sequencing by synthesis," a principle used by Roche-454 <a href="/facts/Pyrosequencing/r7py9uUR">pyrosequencing</a> (introduced in 2005, generating millions of 200-400bp reads in 2009), and the <a href="/facts/Solexa/0nofTkVp">Solexa</a> system (now owned by Illumina) (introduced in 2006, generating hundreds of millions of 50-100bp reads in 2009)
These methods have reduced the cost from $0.01/base in 2004 to nearly $0.0001/base in 2006 and increased the sequencing capacity from 1,000,000 bases/machine/day in 2004 to more than 5,000,000,000 bases/machine/day in 2009. Over 30 publications exist describing its use first for nucleosome positioning from Valouev et al., transcriptional profiling or strand sensitive RNA-Seq with Cloonan et al., single cell transcriptional profiling with Tang et al. and ultimately human resequencing with McKernan et al.
The method used by this machine (sequencing-by-ligation) has been reported to have some issue sequencing palindromic sequences.

ABI Solid Sequencing open-in-new

ABI Solid Sequencing