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Nile blue
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<p>Nile blue (or Nile blue A) is a <a href="/facts/Staining_(biology)/jqGqWEE1">stain</a> used in <a href="/facts/Biology/1M1ee3pB">biology</a> and <a href="/facts/Histology/yrI90zlU">histology</a>. It may be used with live or fixed cells, and imparts a blue colour to cell <a href="/facts/Nucleus_(cell)/D7sFRa3c">nuclei</a>. </p><p>It may also be used in conjunction with fluorescence microscopy to stain for the presence of <a href="/facts/Polyhydroxybutyrate/vfzMPy52">polyhydroxybutyrate</a> granules in prokaryotic or eukaryotic cells. Boiling a solution of Nile blue with <a href="/facts/Sulfuric_acid/Wom6fymJ">sulfuric acid</a> produces <a href="/facts/Nile_red/VWjrel69">Nile red</a> (Nile blue oxazone). </p>