Affinity chromatography

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Affinity chromatography is a method of separating a <a href="/facts/Biomolecule/DGvFzN7R">biomolecule</a> from a mixture, based on a highly specific <a href="/facts/Molecular_binding/cBnorUC5">macromolecular binding</a> interaction between the biomolecule and another substance.  The specific type of binding interaction depends on the biomolecule of interest; <a href="/facts/Antigen/9JlAernG">antigen</a> and <a href="/facts/Antibody/MX8pdTdP">antibody</a>, <a href="/facts/Enzyme/DSI1Fh7y">enzyme</a> and <a href="/facts/Substrate_(biochemistry)/6MnaV19h">substrate</a>, <a href="/facts/Biochemistry_receptor/tjTAC76a">receptor</a> and <a href="/facts/Ligand_(biochemistry)/44QLL4eU">ligand</a>, or <a href="/facts/Protein/Jxmagu3E">protein</a> and <a href="/facts/Nucleic_acid/2tTgcI0v">nucleic acid</a> binding interactions are frequently exploited for isolation of various biomolecules.  Affinity chromatography is useful for its high selectivity and <a href="/facts/Resolution_(chromatography)/ybw7lEYA">resolution</a> of separation, compared to other chromatographic methods.
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Affinity chromatography open-in-new

Affinity chromatography