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Reference.org
G-less cassette
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<p>The G-less cassette transcription assay is a method used in <a href="/facts/Molecular_biology/YgqU35ub">molecular biology</a> to determine <a href="/facts/Promoter_(genetics)/YDYBzLfg">promoter</a> strength <i>in vitro</i>. The technique involves quantification of an mRNA product with the use of a plasmid. The G-less cassette is part of a pre-constructed vector, usually containing a <a href="/facts/Multiple_cloning_site/DUf4uUCh">multiple cloning site</a> (MCS) upstream of the cassette. For this reason, promoters of interest can be inserted directly into the MCS to ultimately measure the accuracy and efficiency of a promoter in recruiting transcription machinery. </p>