In enzymology, a 4-hydroxyacetophenone monooxygenase (EC 1.14.13.84) is an enzyme that catalyzes the chemical reaction:

The 4 substrates of this enzyme are (4-hydroxyphenyl)ethan-1-one, NADPH, H+, and O2, whereas its 3 products are hydroquinone acetate ester, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is (4-hydroxyphenyl)ethan-1-one,NADPH:oxygen oxidoreductase (ester-forming). This enzyme is also called HAPMO. This enzyme participates in bisphenol a degradation.

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• Kamerbeek NM, Olsthoorn AJ, Fraaije MW, Janssen DB (2003). "Substrate specificity and enantioselectivity of 4-hydroxyacetophenone monooxygenase". Appl. Environ. Microbiol. 69 (1): 419–26. doi:10.1128/AEM.69.1.419-426.2003. PMC 152415. PMID 12514023.