Alsever's solution is a saline liquid used to prevent coagulation of blood. It is composed of 2.05% dextrose, 0.8% sodium citrate, 0.055% citric acid, and 0.42% sodium chloride. For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution. This solution is used to study in vitro anti-inflammatory activity of crude drugs by the human red blood cell stabilization method. It is also used to preserve blood cells from other sources.
The test was invented in 1941 by the American Hematologist, John Bellows Alsever (1908–1978).
References
Alsever, J. B., & Ainslie, R. B. (1941). A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. NY State J. Med, 41, 126-131. ↩
Campbell, D. H.; Garvey, J. S.; Cremer, N. E.; Sussdorf, D. H. (1963). Methods in Immunology. A Laboratory Text for Instruction and Research. New York: W. A. Benjamin, Inc. p. 244. ↩
Gandhisan R, Thamaraichelvan A, Baburaj. Antiinflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitotherapia 1991; 62: 82-83. ↩
Schjerning-Thiesen, Knud (2009). "Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution". Acta Pathologica et Microbiologica Scandinavica. 32 (1): 198–203. doi:10.1111/j.1699-0463.1953.tb00242.x. PMID 13050415. /wiki/Doi_(identifier) ↩
L.S.A., List of C.F.R. Sections Affected. 2003. p. 827. https://books.google.com/books?id=OLd91TkawxMC&pg=PA827 ↩
"John Bellows Alsever". TheFreeDictionary.com. https://medical-dictionary.thefreedictionary.com/John+Bellows+Alsever ↩