Night Owl may refer to:

• An owl
• Night owl (person), a person who is most active or creative during the night
• Night Owl (film), a 1993 film by Jeffrey Arsenault
• "Night Owl", a Bangkok Post newspaper column by Bernard Trink
• Night Owl (book), a 2021 political memoir written by Anna Mae Yu Lamentillo
• Night Owl (album), a 1979 album by Gerry Rafferty
• "Night Owl" (Gerry Rafferty song), 1979
• "Night Owl" (James Taylor song), 1966 song recorded by James Taylor, Carly Simon and others
• Night Owl (train), a former Amtrak passenger train between Boston and Washington, D.C.
• The Night Owl (album), 1987 Gregg Karukas album
• The Night Owl (1926 film), American silent film
• The Night Owl (2022 film), a South Korean period thriller drama film
• Night Owl Cinematics, Singaporean YouTube channel and media company
• NightOwl Convenience Stores, Australian store chain
• Keyfax Nite-Owl, a service that provided news, weather, sports, and entertainment information on WFLD Channel 32 in Chicago
• "Night Owl", a song by We Stood Like Kings
• The Night Owl Cafe, a Greenwich Village music club which is referenced in the 1967 song "Creeque Alley"
• A "nightowl" lunch wagon

Night Owls may refer to:

• The Night Owls (song), a song by Little River Band from the 1981 album Time Exposure
• Night Owls (vocal group), a Vassar College a cappella group
• Night Owls (1928 film), a Krazy Kat animated film
• Night Owls (1930 film), a Laurel and Hardy film
• Night Owls (2015 film), starring Adam Pally and Rosa Salazar
• Night Owls (album), a 1990 album by Vaya Con Dios
• Night Owls, a 2024 album by American punk rock band The Dollyrots
• Night Owls, a 2015 novel by Jenn Bennett
• The Night Owls (webcomic), a line of comics by Zuda comics
• Nanaimo NightOwls, baseball team in the West Coast League