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In enzymology, an urethanase (EC 3.5.1.75) is an enzyme that catalyzes the chemical reaction
urethane + H2O ⇌ {\displaystyle \rightleftharpoons } ethanol + CO2 + NH3Thus, the two substrates of this enzyme are urethane and H2O, whereas its 3 products are ethanol, CO2, and NH3.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is urethane amidohydrolase (decarboxylating). This enzyme is also called urethane hydrolase.
- Kobashi K, Takebe S, Sakai T (May 1990). "Urethane-hydrolyzing enzyme from Citrobacter sp". Chem. Pharm. Bull. 38 (5). Tokyo: 1326–8. doi:10.1248/cpb.38.1326. PMID 2393957.