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Optogenetic methods to record cellular activity
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<h2 id="history">History</h2> <p>The first experiments to measure intracellular calcium levels via protein expression were based on <a href="/facts/Aequorin/TqKNvp2a">aequorin</a>, a bioluminescent protein from the jellyfish <a href="/facts/Aequorea_victoria/y8Rk5gul"><i>Aequorea</i></a>. To produce light, however, this enzyme needs the 'fuel' compound <a href="/facts/Coelenterazine/URDjjkRd">coelenteracine</a>, which has to be added to the preparation. This is not practical in intact animals, and in addition, the temporal resolution of bioluminescence imaging is relatively poor (seconds-minutes). The first <a href="/facts/Calcium_imaging/3Y85smYK">genetically encoded fluorescent calcium indicator</a> (GECI) to be used to image activity in an animal was <a href="/facts/Cameleon_(protein)/hHqYSEkH">cameleon</a>, designed by Atsushi Miyawaki, <a href="/facts/Roger_Y._Tsien/TMl3LvSN">Roger Tsien</a> and coworkers in 1997.[1] Cameleon was first used successfully in an animal by Rex Kerr, William Schafer and coworkers to record from neurons and muscle cells of the <a href="/facts/Nematode/yLMJ43Eu">nematode</a> <i>C. elegans</i>.<a class="footnote-ref" id="fnref:1" href="#fn:1"><sup>1</sup></a> Cameleon was subsequently used to record neural activity in flies<a class="footnote-ref" id="fnref:2" href="#fn:2"><sup>2</sup></a> and zebrafish.<a class="footnote-ref" id="fnref:3" href="#fn:3"><sup>3</sup></a> In mammals, the first GECI to be used <i>in vivo</i> was <a href="/facts/GCaMP/pAEWuMeT">GCaMP</a>,<a class="footnote-ref" id="fnref:4" href="#fn:4"><sup>4</sup></a> first developed by Junichi Nakai and coworkers in 2001.<a class="footnote-ref" id="fnref:5" href="#fn:5"><sup>5</sup></a> GCaMP has undergone numerous improvements, notably by a team of scientists at the <a href="/facts/Janelia_Farm_Research_Campus/ysjcMWz9">Janelia Farm Research Campus</a> (GENIE project, <a href="/facts/Howard_Hughes_Medical_Institute/YEu3w6qy">HHMI</a>), and GCaMP6<a class="footnote-ref" id="fnref:6" href="#fn:6"><sup>6</sup></a> in particular has become widely used in neuroscience. Very recently, <a href="/facts/G_protein-coupled_receptor/0arUMkQc">G protein-coupled receptors</a> have been harnessed to generate a series of highly specific indicators for various <a href="/facts/Neurotransmitter/LlpYFSU3">neurotransmitters</a>.<a class="footnote-ref" id="fnref:7" href="#fn:7"><sup>7</sup></a><a class="footnote-ref" id="fnref:8" href="#fn:8"><sup>8</sup></a> </p> <h2 id="design-principles">Design principles</h2> <p>Genetically encoded sensors are <a href="/facts/Fusion_protein/LCzaDz15">fusion proteins</a>, consisting of a ligand binding domain (sensor) and a <a href="/facts/Fluorescent_protein/WQv6UQ7T">fluorescent protein</a>, attached by a short linker (flexible peptide). When the sensor domain binds the correct ligand, it changes conformation. This movement is transferred to the fluorescent protein and the resulting deformation leads to a change in fluorescence. The efficiency of this process depends critically on the length of the linker region, which has to be optimized in a labor-intensive process. The fluorescent protein is often circularly permuted, i.e. new <a href="/facts/C-terminus/ws4scHgT">C-terminal</a> and <a href="/facts/N-terminus/yKYpuaBJ">N-terminal</a> ends were created. Single-wavelength sensors are easy to use for qualitative measurements, but difficult to calibrate for quantitative measurements of ligand concentration. </p><p>A second class of sensors relies on <a href="/facts/F%C3%B6rster_resonance_energy_transfer/6dJTdAC6">Förster resonance energy transfer</a> (FRET) between two fluorescent proteins (FP) of different color. The shorter wavelength FP (donor) is excited with blue light from a laser or LED. If the second FP (acceptor) is very close, the energy is transferred to the acceptor, resulting in yellow or red fluorescence. When the acceptor FP moves further away, the donor emits green fluorescence. The sensor domain is typically spliced between the two FPs, resulting in a hinge-type movement upon ligand binding that changes the distance between donor and acceptor. The imaging procedure is more complex for FRET sensors, but the fluorescence ratio can be calibrated to measure the absolute concentration of a ligand. Read-out via <a href="/facts/Fluorescence-lifetime_imaging_microscopy/BM0vs5of">fluorescence lifetime imaging</a> (FLIM) of donor fluorescence is also possible, as the FRET process speeds up the fluorescence decay. </p> <h2 id="advantages-of-optogenetic-sensors">Advantages of optogenetic sensors</h2> <ul><li>can be targeted to specific classes of cells (e.g. <a href="/facts/Astrocyte/PBUuHKzS">astrocytes</a> or <a href="/facts/Pyramidal_cell/T8MTBq8b">pyramidal cells</a>). This allows for optical read-out without spatial resolution, e.g. <a href="/facts/Photometry_(optics)/eo6J7qj3">fiber photometry</a> from deep brain areas.<a class="footnote-ref" id="fnref:9" href="#fn:9"><sup>9</sup></a></li> <li>can be targeted to sub-cellular compartments (e.g. synapses, <a href="/facts/Organelle/vhDFnGbs">organelles</a>, nucleus) by fusing the indicator protein with specific anchoring domains, retention signals or <a href="/facts/Intrabody_(protein)/FjCNkBKh">intrabodies</a>.</li> <li>work in a variety of species (<a href="/facts/Nematode/yLMJ43Eu">nematodes</a>, insects, fish, mammals) and in <a href="/facts/Cell_culture/7Qhu13D9">cell culture</a> systems (<a href="/facts/Plate_reader/v7eN64Rd">FLIPR assay</a>)</li> <li>can be delivered by viral vectors (e.g. <a href="/facts/Viral_vector/sqkIdcqo">rAAV</a>)</li> <li>can be used to record the activity of thousands of neurons at the same time <a class="footnote-ref" id="fnref:10" href="#fn:10"><sup>10</sup></a></li></ul> <h2 id="drawbacks-limitations">Drawbacks, limitations</h2> <ul><li>will buffer the measured ion or protein, potentially interfering with cellular signaling</li> <li>are subject to <a href="/facts/Photobleaching/bCzDH8c1">photobleaching</a>, compromising long-term measurements</li> <li>can be toxic when expressed at very high concentration</li> <li>require highly sensitive cameras or <a href="/facts/Confocal_microscopy/Gh7pY9Cl">laser scanning microscopes</a></li> <li>some <a href="/facts/G_protein-coupled_receptor/0arUMkQc">GPCR</a>-based sensors are sensitive to <a href="/facts/Polarization_(waves)/fP33m0eD">polarization</a><a class="footnote-ref" id="fnref:11" href="#fn:11"><sup>11</sup></a></li> <li>most indicators are green fluorescent, making it difficult to measure several cellular parameters simultaneously (<a href="/facts/Multiplexing/YE7hK8cw">multiplexing</a>).</li></ul> <h2 id="classes-of-genetically-encoded-indicators">Classes of genetically encoded indicators</h2> <p>Indicators have been designed to measure ion concentrations, membrane potential, neurotransmitters, and various intracellular signaling molecules. The following list provides only examples for each class; many more have been published. </p> <h3>Intracellular signaling</h3> <ul><li><a href="/facts/Genetically_encoded_calcium_sensor/3Y85smYK">Genetically encoded calcium indicators</a> (GECI): A large class of tools, based on natural calcium binding proteins (<a href="/facts/Calmodulin/CbEnEYHE">calmodulin</a>, <a href="/facts/Troponin/gQdzvCCb">troponin</a>). Different affinities, kinetics, and colors (green, red) available. Read-out via fluorescence intensity (single wavelength indicators), <a href="/facts/F%C3%B6rster_resonance_energy_transfer/6dJTdAC6">FRET</a> or <a href="/facts/Bioluminescence_resonance_energy_transfer/6dJTdAC6">BRET</a>. Have been targeted to various organelles. Current version: JGCaMP8<a class="footnote-ref" id="fnref:12" href="#fn:12"><sup>12</sup></a></li> <li>Genetically encoded <a href="/facts/Chloride/m9NUaUDR">chloride</a> indicators: Clomeleon<a class="footnote-ref" id="fnref:13" href="#fn:13"><sup>13</sup></a></li> <li>Genetically encoded <a href="/facts/Potassium_chloride/RgT2MdPa">potassium</a> indicators: GINKO2<a class="footnote-ref" id="fnref:14" href="#fn:14"><sup>14</sup></a></li> <li>Genetically encoded indicators for intracellular pH (GEPhI): CypHer<a class="footnote-ref" id="fnref:15" href="#fn:15"><sup>15</sup></a></li> <li><a href="/facts/Genetically_encoded_voltage_indicator/pzvY57EK">Genetically encoded voltage indicators</a> (GEVI): ArcLight<a class="footnote-ref" id="fnref:16" href="#fn:16"><sup>16</sup></a></li> <li>Genetically encoded vesicle fusion sensors: <a href="/facts/Synapto-pHluorin/3QVHvN9T">Synapto-pHluorin</a>, Synaptophysin-pHluorin<a class="footnote-ref" id="fnref:17" href="#fn:17"><sup>17</sup></a></li> <li>Genetically encoded cAMP sensors: EPAC<a class="footnote-ref" id="fnref:18" href="#fn:18"><sup>18</sup></a></li> <li>Genetically encoded <a href="/facts/Adenosine_triphosphate/nB9bwgcU">ATP</a> sensors: QUEEN-37C<a class="footnote-ref" id="fnref:19" href="#fn:19"><sup>19</sup></a></li> <li>Genetically encoded <a href="/facts/Kinase/dhlHpjnD">kinase</a> activity sensors: CaMui,<a class="footnote-ref" id="fnref:20" href="#fn:20"><sup>20</sup></a> <a href="/facts/SmURFP/VrQXSVhU">SmURFP</a></li> <li>Genetically encoded Small G-protein sensors: FRas<a class="footnote-ref" id="fnref:21" href="#fn:21"><sup>21</sup></a></li></ul> <h3>Neurotransmitters and other extracellular signals</h3> <ul><li><a href="/facts/Glutamate-sensitive_fluorescent_reporter/5XP68g9X">Genetically encoded glutamate sensors:</a> GluSnFR<a class="footnote-ref" id="fnref:22" href="#fn:22"><sup>22</sup></a></li> <li>Genetically encoded <a href="/facts/Gamma-Aminobutyric_acid/vRTeQQUG">GABA</a> sensors: iGABASnFR<a class="footnote-ref" id="fnref:23" href="#fn:23"><sup>23</sup></a></li> <li>Genetically encoded <a href="/facts/Dopamine/5gNy8Xkd">dopamine</a> sensors: dLight1,<a class="footnote-ref" id="fnref:24" href="#fn:24"><sup>24</sup></a> GRAB-DA<a class="footnote-ref" id="fnref:25" href="#fn:25"><sup>25</sup></a></li> <li>Genetically encoded <a href="/facts/Serotonin/EzY58q4a">serotonin</a> sensors: GRAB5-HT,<a class="footnote-ref" id="fnref:26" href="#fn:26"><sup>26</sup></a> sDarken,<a class="footnote-ref" id="fnref:27" href="#fn:27"><sup>27</sup></a> iSeroSnFR<a class="footnote-ref" id="fnref:28" href="#fn:28"><sup>28</sup></a></li> <li>Genetically encoded <a href="/facts/Norepinephrine/AjTIC9ox">norepinephrine</a> sensors: GRABNE<a class="footnote-ref" id="fnref:29" href="#fn:29"><sup>29</sup></a></li> <li>Genetically encoded sensor for <a href="/facts/Endocannabinoid_system/eIpB5GFm">endocannabinoid</a> activity: GRABeCB2.0<a class="footnote-ref" id="fnref:30" href="#fn:30"><sup>30</sup></a></li> <li>Genetically encoded sensor for <a href="/facts/Orexin/bHJMxntz">orexin/hypocretin neuropeptides</a>: OxLight1<a class="footnote-ref" id="fnref:31" href="#fn:31"><sup>31</sup></a></li> <li>Genetically encoded sensor for <a href="/facts/Lactate_shuttle_hypothesis/D1xK4UAR">lactate</a>: eLACCO1.1<a class="footnote-ref" id="fnref:32" href="#fn:32"><sup>32</sup></a></li></ul> <h2 id="further-reading">Further reading</h2> <p>A recent review of <a href="https://onlinelibrary.wiley.com/doi/10.1111/jnc.16045">GPCR-based genetically encoded fluorescent indicators</a> for neuromodulators <a class="footnote-ref" id="fnref:33" href="#fn:33"><sup>33</sup></a> </p> <h2 id="external-links">External links</h2> <ul><li><a href="https://biosensordb.ucsd.edu/index.php">Fluorescent Biosensor Database</a>, a fairly complete searchable list of published sensors and their basic properties, maintained by Jin Zhang's lab at UCSD.<a class="footnote-ref" id="fnref:34" href="#fn:34"><sup>34</sup></a></li> <li><a href="https://www.addgene.org/fluorescent-proteins/biosensors/">Fluorescent Biosensors</a> available on <a href="/facts/Addgene/DcVpNvlL">Addgene</a>, a nonprofit plasmid repository.</li></ul> <h2 id="references">References</h2> <ol> <li id="fn:1"><p>Kerr R, Lev-Ram V, Baird G, Vincent P, Tsien RY, Schafer WR (June 2000). 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