Puncture, punctured or puncturing may refer to:

• a flat tyre in British English (US English "flat tire" or just "flat")
• a penetrating wound caused by pointy objects as nails or needles
• Lumbar puncture, also known as a spinal tap
• Puncture (band), an English punk band
• Puncture (film), a 2011 American film starring Chris Evans
• Puncture (topology), the removal of a finite set of points from a manifold
• Punctured neighbourhood, in general topology
• in coding theory, a punctured code, in which some of the bits of the data stream have been removed
• Pneumothorax, also known as punctured lung